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OfflineDeadPhan
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Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
Running jars (g2g) too far?
    #18731356 - 08/20/13 08:55 AM (10 years, 5 months ago)

I've read about this before.  My jars I have now must have been from the same batch that I've since grain to grained maybe 7 or so times now. They seem to be contaming easy, especially with bacteria.  And if they don't are very weak. Would this be cause by running them too far?  Otherwise I'm once again worried on my FH. I do however have newly inoculated jars on those way so hopefully those turn out and ill know for sure.


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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Offlinemiw4life
The Shroomer
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Registered: 07/04/13
Posts: 192
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Re: Running jars (g2g) too far? [Re: DeadPhan]
    #18731528 - 08/20/13 09:57 AM (10 years, 5 months ago)

well the main thing you can do to get them to be more potent is definatly how you take care of there. the more love you give them the better they will be. as for the contam part. go over your procedure step by step and think about your sterilization. something may need to be done better.


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"The answer to every question, is the question itself"


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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Running jars (g2g) too far? [Re: miw4life]
    #18731547 - 08/20/13 10:05 AM (10 years, 5 months ago)

Quote:

miw4life said:
well the main thing you can do to get them to be more potent is definatly how you take care of there. the more love you give them the better they will be. as for the contam part. go over your procedure step by step and think about your sterilization. something may need to be done better.




I think this is too advanced for either of us to contribute.


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Offlinemiw4life
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Registered: 07/04/13
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Re: Running jars (g2g) too far? [Re: Pestile]
    #18731557 - 08/20/13 10:09 AM (10 years, 5 months ago)

im just trying to help man with what I know for sure. and im just trying to get rated.


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"The answer to every question, is the question itself"


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OfflineBloodKil
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Registered: 03/16/13
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Re: Running jars (g2g) too far? [Re: miw4life]
    #18731699 - 08/20/13 10:57 AM (10 years, 5 months ago)

Get going with some agar deadphan.  Honestly there is no need to continue g2g for that long when a perfectly viable and nearly as quick (if not quicker) alternative is around.

At most I would do, master - g2g - g2g...  Why risk going any further and running your mycelium till it flops out.

With agar you could stagger it like this...

Master slant - plate - bunch of plates
                                        Agar - master grain - g2g - tub/log (whatever you are growing)
                                                      Agar - master grain- g2g - t/l
                                                                  Agar - master grain - g2g - t/l

Repeat that for as many plates as you made, staggering for a continual harvest...  just restart the master slant to plate step when you run down to only a couple of plates left (this again depends on size of plate, amount of masters you make each run, etc...)

This is from masters inoculated with a mycelium scraping (I find better distribution and recovery as opposed to a wedge) from a 65mm plate...  I nock up 2 masters from each plate.  The pictures below is 2 days after inoculation (keep in mind it's oysters so they do recover and spread a bit faster than cubes...  I'd say give an extra 1- 1.5 days extra time for them to be at this point from a cube agar scraping - master)



These jars are usually done in ~5 days by simply scraping mycelium from the agar, inoculation, and shaking the shit out of them...

No need to stretch out g2g when you can move that quick with "fresher" mycilia.


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