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OfflinebEelzeBosS
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Registered: 06/15/13
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Isolation question
    #18729377 - 08/19/13 07:42 PM (10 years, 5 months ago)

I have 3 tubs going right now, all spawned from the same master jar. The first flush for all of them was extremely thick but the fruits were only 1-2" tall. The 2nd and 3rd flushes however are putting out some monsters. From what I understand it's because they are different strains since the master spawn jar was from a MS LC. How would I go about isolating the strain from the 2nd and 3rd flushes so I can always get these bigger fruits? I still have some of the LC they came from, would I just drop some on a petri then transfer some of the slowest growing myc to try and isolate since the big fruits are coming in later?


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Invisiblemushmagic
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Re: Isolation question [Re: bEelzeBosS]
    #18729418 - 08/19/13 07:50 PM (10 years, 5 months ago)

You would need to isolate multiple different sets of genetics on agar and test them all out seperately and then choose the genetics you're looking for. Or you can try cloning but cloning will still have multiple strains present so it can't be considered an isolate but you can still further isolate a clone. Clones from earlier flushes are more vigorous though.


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OfflinebEelzeBosS
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Re: Isolation question [Re: mushmagic]
    #18729474 - 08/19/13 08:02 PM (10 years, 5 months ago)

So basically if you get some good fruits it will take months to ensure you get them consistently...


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OfflineSgt. Pepper
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Re: Isolation question [Re: bEelzeBosS]
    #18729528 - 08/19/13 08:14 PM (10 years, 5 months ago)

I'm currently researching, but am still unfarmiliar with agar work and isolation so bare with me. What you can do is drop a piece of the inner stem of one of the shrooms you like on agar and wait for it to grow out. You could isolate it further by taking the most rhizomorphic and fastest growing of the sectors and putting it on a new plate. That way you will have a single strain. Another, simpler, option is to take the inner stem piece and put it in a grain jar. You won't have a single strain, but you know you will have quality genetics still. Hope that helped.


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Offlinecubenpete
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Re: Isolation question [Re: Sgt. Pepper]
    #18729689 - 08/19/13 08:44 PM (10 years, 5 months ago)

Not strain, monoculture.

Agar is a much better way than throwing a possibly contaminated piece of flesh into a grain jar. With agar you can avoid contams by cutting a wedge far away from the invader(s) and introducing the agar wedge to a new petri. I would bet flesh in a grain jar would fail more times than not (next to impossible to remove cotams from a jar, IME).


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Invisiblemushmagic
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Re: Isolation question [Re: cubenpete]
    #18730075 - 08/19/13 10:04 PM (10 years, 5 months ago)

Quote:

cubenpete said:
Not strain, monoculture.




A monoculture actually is a single strain.


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InvisiblePastywhyteMDiscord
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Re: Isolation question [Re: Sgt. Pepper]
    #18730097 - 08/19/13 10:08 PM (10 years, 5 months ago)

Quote:

mushmagic said:
A monoculture actually is a single strain.




:whathesaid:

Quote:

Sgt. Pepper said:
Another, simpler, option is to take the inner stem piece and put it in a grain jar.




I wouldn't do that :shrug: use agar, that's what its for.


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InvisibleFrankHorrigan
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Re: Isolation question [Re: Pastywhyte] * 1
    #18730203 - 08/19/13 10:33 PM (10 years, 5 months ago)

Don't clone second flush fruits.

Those are always going to be bigger.

Clone first flush fruits that grow quickly. These are your best fruiters.

Second-flush and later clones are poor performing pieces of shit that get thrown in the trash.

I challenge anyone to share a time they found that to not be the case...because I sure haven't :shrug:


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Offlinecubenpete
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Re: Isolation question [Re: Sgt. Pepper]
    #18730226 - 08/19/13 10:38 PM (10 years, 5 months ago)

Quote:

Pastywhyte said:
Quote:

mushmagic said:
A monoculture actually is a single strain.





OP said:

Quote:

Sgt. Pepper said:
What you can do is drop a piece of the inner stem of one of the shrooms you like on agar and wait for it to grow out. You could isolate it further by taking the most rhizomorphic and fastest growing of the sectors and putting it on a new plate. That way you will have a single strain.




This is not a strain, it is a cloning of desirable characteristics within a single fruit of a mushroom variety. A Monoculture.... Right?  :smirk:


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Invisiblemushmagic
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Re: Isolation question [Re: cubenpete]
    #18730399 - 08/19/13 11:24 PM (10 years, 5 months ago)

Ok my bad, I think I understand what you were getting at now. Correct a clone will not have only one strain present but will have multiple. It can be further isolated to contain only one present strain and then would be a monoculture/isolate. I just thought you were saying a monoculture wasn't a single strain.


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OfflineRogerRabbitM
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Re: Isolation question [Re: bEelzeBosS]
    #18731085 - 08/20/13 06:46 AM (10 years, 5 months ago)

Quote:

chaotic646 said:
How would I go about isolating the strain from the 2nd and 3rd flushes so I can always get these bigger fruits?




I see no point in isolating a strain which takes twice as long to deliver fewer fruits.

In addition, smaller mushrooms are more potent.  I'd isolate the first flush to get a quick, potent strain.
RR


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OfflinebEelzeBosS
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Re: Isolation question [Re: FrankHorrigan]
    #18731467 - 08/20/13 09:34 AM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
Don't clone second flush fruits.

Those are always going to be bigger.

Clone first flush fruits that grow quickly. These are your best fruiters.

Second-flush and later clones are poor performing pieces of shit that get thrown in the trash.






Then how do people (you included) get these tubs full of 6"+ fruits all the time? Do you guys just get lucky with a 1st flush of monsters then clone them?

Quote:

RogerRabbit said:

I see no point in isolating a strain which takes twice as long to deliver fewer fruits.

In addition, smaller mushrooms are more potent.  I'd isolate the first flush to get a quick, potent strain.
RR



I understand the small ones are more potent, but Im looking for weight and not having to spend hours on end harvesting these little guys...seems like if they were all big it would be a LOT easier to harvest.


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