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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
How to clean (NOT sterilize) contaminated PLASTIC petris?
    #18728843 - 08/19/13 05:50 PM (10 years, 5 months ago)

I've only got a few petri dishes from an old project and no money to buy new ones. The ones I have are plastic and since they've been sitting around have been overtaken by some kind of black mold. I could go scrape them into the compost, but my worry is that the spores will get all over me and thus I'll track them back into the house. With contaminated jars, as someone else suggested, I pressure cook them and then empty them out. The plastics cannot handle the pressure cooker however and melt, so I'm looking for a way to get the dishes clean without getting the spores everywhere. Does anyone have any ideas?

The best idea I have atm is to maybe leave them in the sun until the dishes dry out. I'm not sure how airborn the spores are, but perhaps this would, in addition to killing the mold, trap most of the spores within the mold due to the dry process. My other idea is to open them up under water and then simply hose/wash my hands off afterwords, hopefully they'd all just be stuck within the solution. The last idea I had, which is a pita, is to take my clothes off and throw them in the wash and hose down outside. I used to have a big contamination problem and have cleaned my whole house so I'm doing everything I can to not recontaminate the place. I used to have a hard time even growing out a monotub without a contamination and now I'm at the point where I can do g2g transfers no problem, even leave jars exposed to the open air and not have an issue, I'd really like to keep it this way.


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Invisibleanne halonium
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Registered: 05/07/13
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: krypto2000]
    #18728857 - 08/19/13 05:53 PM (10 years, 5 months ago)

you really cant effectively clean them.


--------------------
:aliendance:


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: krypto2000]
    #18728860 - 08/19/13 05:54 PM (10 years, 5 months ago)

Could you submerge them into a bucket of hot water/10% Clorox without opening them?  If you have to save them, good luck.


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Offlinekrypto2000
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: anne halonium]
    #18728866 - 08/19/13 05:55 PM (10 years, 5 months ago)

What do you mean by effectively? Without getting the spores everywhere? I have my own sterilization methods which work well enough, I'm just trying to clean them, as the title states, not sterilize them.


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Offlinekrypto2000
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: HypnotoadCroaked]
    #18728875 - 08/19/13 05:56 PM (10 years, 5 months ago)

Quote:

Whippy said:
Could you submerge them into a bucket of hot water/10% Clorox without opening them?  If you have to save them, good luck.




Yeah, that's probably my best bet. I'll try that tomorrow unless anyone suggests something better. I really need to get some glass petris, I hate these plastic ones tbh.


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Invisibleanne halonium
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: krypto2000]
    #18728879 - 08/19/13 05:57 PM (10 years, 5 months ago)

if they are clean, what value would they have?
id suggest , less than price of the bleach or time.


--------------------
:aliendance:


Edited by anne halonium (08/19/13 05:58 PM)


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OfflineKizzle
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: anne halonium]
    #18728881 - 08/19/13 05:58 PM (10 years, 5 months ago)

Open them in soapy water with gloves on.

I'm curious why you'd want to clean them if you can't sterilize them for reuse anyway?


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Invisibleanne halonium
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: Kizzle]
    #18728891 - 08/19/13 06:01 PM (10 years, 5 months ago)

for future reference,
polycarbonate culture bottles.

a plastic, you can PC...........




PP5 cups, also work well.


--------------------
:aliendance:


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Offlinekrypto2000
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: anne halonium]
    #18729011 - 08/19/13 06:27 PM (10 years, 5 months ago)

I can at least partially sterilize them. I was thinking of maybe boiling them, possibly in a mild bleach solution, and then turning them upside down in my glovebox to dry. They don't necessarily need to be completely sterile anyway to work with, unlike with jars you just work around the contaminants because they're normally fairly visible. Part of what I wanted to use them for also is to test the cleanliness/spore count in various rooms in my house, so so long as the dishes are equally sterile (or unsterile) I'll be able to compare them to one another.


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Offlinekrypto2000
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: anne halonium]
    #18729022 - 08/19/13 06:28 PM (10 years, 5 months ago)

Quote:

anne halonium said:
if they are clean, what value would they have?
id suggest , less than price of the bleach or time.





This doesn't make sense. If they're clean as opposed to sterile you mean? I'm just looking for a way to clean them first then I'll worry about sterilizing them.


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Invisibleanne halonium
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: krypto2000]
    #18729051 - 08/19/13 06:33 PM (10 years, 5 months ago)

lol.
those things are PET,
they are made for labs to be disposable, and when processed be destroyed so they cant be re used.
otherwise, fools would dumpster dive bio labs and resell em.

those things are not made to be multi use for boomer peeps.
they are made for pro labs, as a inexpensive disposable safe alt to glass.


there is no reliable way to re sterlize PET.....
unless ya have irradiating equip. you do not.

know your plastics.
for the effort , you can raid a recycle bin for PP5,
and have something of real use.

all plastics , can be identified by code,
and ,found on wikipedia.

i sympathize with your finances, but,
there are better solutions than re using PET dishes.

keep in mind the bigger picture,
your on a grow forum, asking how to save 3$ worth of 2 cent plastics.
the onus of "making sense / cents" would be on you.



--------------------
:aliendance:


Edited by anne halonium (08/19/13 06:50 PM)


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OfflineKizzle
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: anne halonium]
    #18729888 - 08/19/13 09:30 PM (10 years, 5 months ago)

I'm guessing if a petri can't stand up to PC temperatures it probably can't withstand boiling/steaming temperatures either in which case the only way I know of to sterilize them would be with radiation or a chemical sterilizer. Neither of which would be practical.

I suspect if you were able to at least thoroughly pasteurize them, which can be done at lower temperatures, and prevent recontamination in the process you might to get away with reusing them with antibiotic agar. That stil seems like a lot of work for a cheap plastic dish.


Edited by Kizzle (08/19/13 09:42 PM)


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Invisibleanne halonium
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: Kizzle]
    #18729946 - 08/19/13 09:41 PM (10 years, 5 months ago)

PET plates , unfortunately ,
are engineered to be like tampons.
there is no practical recovery and re use option.


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:aliendance:


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OfflineSpencerPhillips
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: anne halonium]
    #18730066 - 08/19/13 10:02 PM (10 years, 5 months ago)

anne i've had tampon popsicles, it's a form of recyclign them


--------------------
Come on put it up! Woooo!


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OfflinePhosCap
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: krypto2000]
    #18730113 - 08/19/13 10:12 PM (10 years, 5 months ago)

If it were me..
Open them under hot soapy water, and scrap all the shit off. Use SOS pads, then get right in there with a clean dish rag. I would rinse each one under running hot water 2-3min per. Then I would drop some Dr Bronner soap(or any other high concentration oil) in there, pour boiling water or scolding hot water, and let sit for a a 1-3 days. Then rinse and scrub with clean rag.


--------------------
Psychoactive Fungi of the World        TLs            Franks teks and Tips        Stonesun's..
Spitballs Quick Rye Prep


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InvisibleFrankHorrigan
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: krypto2000]
    #18730190 - 08/19/13 10:30 PM (10 years, 5 months ago)

Quote:

krypto2000 said:
I'm just looking for a way to clean them first then I'll worry about sterilizing them.




Then just wash them with warm soapy water like anything else :facepalm:

You won't be able to properly aterilize them however.

Those plastic petris melt in a PC.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


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Invisibleanne halonium
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: FrankHorrigan]
    #18731222 - 08/20/13 07:50 AM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
Quote:

krypto2000 said:
I'm just looking for a way to clean them first then I'll worry about sterilizing them.




Then just wash them with warm soapy water like anything else :facepalm:






:facepalm:

so, ill pencil ya in frank ,
for washing unknown contams in the sink, to save a 3 cent dish?

what happens when he hits a plate with crypotcoccus?
should he hold his breath? or should he send ya the hospital bill.

haphazard handling of contams,
is never a good idea.
some are quite toxic.

allow me to be be unequivical.
contamed cultures, should be destroyed, and not played with.
the standard should NOT be, save a 3 cent contam dish at all costs.

i appreciate in this economy , some are broke.
its not an excuse to reduce home bio work, to the lowest common denominator, and ,short circuit lab safety 101.

as said before, for the effort, one can snag PP5 containers,
and have a response of value, without spreading contams thru the sink area.

fact is, none of ya have a factual grip, whats on those plates.
i offer its irresponsible to handle unknown contams in a cavalier way.

pros should not reduce contams to a bucket slosh and a soapy rag,
noobs should rise to best handling practice, and safety standards.


--------------------
:aliendance:


Edited by anne halonium (08/20/13 07:55 AM)


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Invisibleanne halonium
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: SpencerPhillips]
    #18731258 - 08/20/13 08:05 AM (10 years, 5 months ago)

Quote:

Sylus said:
anne i've had tampon popsicles, it's a form of recyclign them




oh, and im assuming most growers,
arent vampires needing a snack pack,
and, their GF's dont have STD's...........
:facepalm:


--------------------
:aliendance:


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OfflineBloodKil
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: anne halonium]
    #18731261 - 08/20/13 08:06 AM (10 years, 5 months ago)

Quote:

anne halonium said:
lol.
those things are PET,
they are made for labs to be disposable, and when processed be destroyed so they cant be re used.
otherwise, fools would dumpster dive bio labs and resell em.

those things are not made to be multi use for boomer peeps.
they are made for pro labs, as a inexpensive disposable safe alt to glass.


there is no reliable way to re sterlize PET.....
unless ya have irradiating equip. you do not.

know your plastics.
for the effort , you can raid a recycle bin for PP5,
and have something of real use.

all plastics , can be identified by code,
and ,found on wikipedia.

i sympathize with your finances, but,
there are better solutions than re using PET dishes.

keep in mind the bigger picture,
your on a grow forum, asking how to save 3$ worth of 2 cent plastics.
the onus of "making sense / cents" would be on you.







:doublefacepalm:

Actually Anne those are likely polystyrene plates (come on get to know your plastics.... geez)

-------

Anyway, they are not autoclaveable, and boiling them in bleach will not only be hazardous to your health krypto, but will also likely deform/melt them...

I've had limited success in the past by simply cleaning them outside with a bucket of water and then washing them with a bleach mixture, before taking them inside and again spraying them with a light (5%) bleach mixture inside of my (sanitized) gb and allowing them to dry face down on some clorox wipes...  After doing this though, I find it makes them a bit more brittle (reaction with bleach and or soap I'd assume) and I ended up cracking a good deal of them just picking them up to fill them...

Like you I eventually plan to go glass, but next time you get some cash going and want some cheapies check out cynmar...  they have 25 stacks for 5 bucks each (even less if you buy quantities) and they have 3 and 4 section stackable for the same price.


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Invisibleanne halonium
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: BloodKil]
    #18731269 - 08/20/13 08:12 AM (10 years, 5 months ago)

get to know your safety practices.

my work with plastics, is well known.
and, so is the proper handling of plastic plates.

ill pencil ya in next to frank for,
" save the 3 cent dish, contam safety be damned"


--------------------
:aliendance:


Edited by anne halonium (08/20/13 08:13 AM)


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OfflineBloodKil
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: anne halonium]
    #18731286 - 08/20/13 08:22 AM (10 years, 5 months ago)

Quote:

anne halonium said:
get to know your safety practices.

my work with plastics, is well known.
and, so is the proper handling of plastic plates.

ill pencil ya in next to frank for,
" save the 3 cent dish, contam safety be damned"





My safety and sterility practices are just fine Anne but thanks for your concern.

Your also well known for spouting off and trying to make others feel stupid or make yourself look superior, while the entire time your just either completely wrong or have nothing at the time to back up your claims...  (I even agree with the general principal of some things such as blue light, but you make it really hard to stand with you on such things the way you present yourself)

Either way you were the one going off on a wild tangent about...  omg guis itz PET PET PET...  but you were wrong.  Sorry if it hurt your feelings, but next time don't attack others if you cannot be bothered to get to know the subject your attacking with

And thanks for putting me next to Frank.  I really like a lot of the work he's done around here. :thumbup:


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Invisiblestonesun
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: krypto2000]
    #18731303 - 08/20/13 08:33 AM (10 years, 5 months ago)

I'm sorry OP, but this is just a really silly idea.
Trash your contaminated plastic dishes.
As others said, contaminants could be hazardous to your health,
can effect your working/living area.
You'd take all these risks in account to "clean" them,
and then you'd fail to properly sterilize them anyway.
There's a reason why they call those dishes "disposable".
They were designed for a SINGLE use.


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Invisibleanne halonium
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: BloodKil]
    #18731312 - 08/20/13 08:37 AM (10 years, 5 months ago)

^ on this, stonesun and i can agree.


as for bloodkil,
im for safety and good lab practice.

im sorry that peeps are offended when i point out their folly.
if i told peeps the easy feel good, guess and hope answer,
id be just like.......

were talking contams and 3 cent plastics.
hardly worth defending poor practice when there are health concerns.

your knowledge of advanced lab skills,
and bio safety is apparent,
its why your in permanent sharpie next to frank.

i could care less your opinion on my demeanor,
i do care, if a noob contams himself or grow,
listening to half baked BS, to save a disposable plastic dish.

pardon me.

become aware of the hazards of contams.
your grow will be better, and your health will last longer.

get a liver scan blood,
something tells me youve been handing contams poorly.

i pop up on these threads, not for my health,
but because im sick of seeing peeps advise noobs in risky ways.
if we lose growers to safety errors, or lose grows to contams,
the community is diminished.

standard should be excellence, not cost expedience over safety.


--------------------
:aliendance:


Edited by anne halonium (08/20/13 08:45 AM)


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Offlinekrypto2000
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: anne halonium]
    #18732456 - 08/20/13 01:56 PM (10 years, 5 months ago)

Ok, I'm throwing them out.


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InvisibleStygianKnight
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: krypto2000]
    #18732506 - 08/20/13 02:08 PM (10 years, 5 months ago)

I oddly agree with Anne.

If you are worried about future contamination they can be PCed to kill the mold before disposal, but this will also deform the plates beyond use (they deform but don't fully melt so there's no mess in your PC but also no reusing them).


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Offlinekrypto2000
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: StygianKnight]
    #18732517 - 08/20/13 02:11 PM (10 years, 5 months ago)

Would that not get spores all over my PCer? I pressure cooker my contaminated jars before dumping them into the compost, but I assume the spores, which again I'm assuming some are still viable, at least endospores, stay in the jars at least. If the petris burst wouldn't I essentially be risking contaminating both my PCer and house seeing as the PCer released steam, and thus spores, into the air? Seems safer to me to just strait throw them out and let the garbage men/city deal with it lol. Maybe tape them shut first.


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Invisibleanne halonium
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Re: How to clean (NOT sterilize) contaminated PLASTIC petris? [Re: krypto2000]
    #18732551 - 08/20/13 02:16 PM (10 years, 5 months ago)

real fast, i have a yacht club dinner, and the taxi is killing me.

fill a big bucket with 10% bleach.
put an old towel in, pull it out, wring it,
and place it over the bucket,
take your contianers of question,
put them in bucket under towel and open under the water line.
they will bubble as they sink
towel keeps in most contams that may escape.
let sit 24 hours, then scoop out stuff in containers,
and wash and dry...........
bleach bucket with all the contams, can now be safely disposed.

this works on about everything but plastic disposable petris


--------------------
:aliendance:


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