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Offlinemicroscopes
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Monotub taking forever to Pin
    #18727347 - 08/19/13 12:22 PM (10 years, 5 months ago)

My Monotub, fully colonized, has been in fruiting conditions (polyfill in holes, light fanning, 12/12 6500k light, for 7 days now. There may be some hypnal knots forming, its hard to see. But 7 days, not even a small pin? Is that normal? All i see right now is some yellow myc metabolites. Humidity is high. Droplets on sides of bin.


Edited by microscopes (08/19/13 12:24 PM)


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OfflineStromriderM
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Re: Monotub taking forever to Pin [Re: microscopes]
    #18727414 - 08/19/13 12:32 PM (10 years, 5 months ago)

Yes normal. Give it a few more days


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OfflineStromriderM
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Re: Monotub taking forever to Pin [Re: Stromrider]
    #18727425 - 08/19/13 12:34 PM (10 years, 5 months ago)

Well yellow metabolites may not be normal. What kind of sub?


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OfflineNakor420
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Re: Monotub taking forever to Pin [Re: Stromrider]
    #18727450 - 08/19/13 12:37 PM (10 years, 5 months ago)

I could personally use pics on this one....:shrug:


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OfflineStromriderM
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Re: Monotub taking forever to Pin [Re: Nakor420]
    #18727461 - 08/19/13 12:40 PM (10 years, 5 months ago)

Quote:

Nakor420 said:
I could personally use pics on this one....:shrug:




Agreed. You sure the metabs you speak of aren't just hyphae? They can have kind of a yellow tint sometimes. Does it look like little blobs or like resin? A pic is worth 1000 words


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Offlinemicroscopes
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Re: Monotub taking forever to Pin [Re: Stromrider]
    #18727528 - 08/19/13 12:56 PM (10 years, 5 months ago)

I'll try to take some pics. Little droplets of yellowish colored liquid.


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Offlinemicroscopes
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Re: Monotub taking forever to Pin [Re: microscopes]
    #18728635 - 08/19/13 05:04 PM (10 years, 5 months ago)

Quote:

Nakor420 said:
I could personally use pics on this one....:shrug:




Here's some pictures.



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Offlinemicroscopes
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Re: Monotub taking forever to Pin [Re: Stromrider]
    #18728968 - 08/19/13 06:16 PM (10 years, 5 months ago)

Quote:

psillyshroomer said:
Well yellow metabolites may not be normal. What kind of sub?




Coir and Verm


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InvisibleSpitballJedi
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Re: Monotub taking forever to Pin [Re: microscopes]
    #18729209 - 08/19/13 07:08 PM (10 years, 5 months ago)

does it have a funky smell?


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OfflineStromriderM
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Re: Monotub taking forever to Pin [Re: SpitballJedi]
    #18729222 - 08/19/13 07:12 PM (10 years, 5 months ago)

That myc does not look normal to me. It is really fluffy and cottony looking not to mention the yellow stuff. I guess you'll just have to wait and see


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Offlinemicroscopes
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Re: Monotub taking forever to Pin [Re: SpitballJedi]
    #18729376 - 08/19/13 07:42 PM (10 years, 5 months ago)

Quote:

SpitballJedi said:
does it have a funky smell?




Nope a strong mushroomy smell.


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Offlinemicroscopes
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Re: Monotub taking forever to Pin [Re: Stromrider]
    #18729384 - 08/19/13 07:43 PM (10 years, 5 months ago)

Quote:

psillyshroomer said:
That myc does not look normal to me. It is really fluffy and cottony looking not to mention the yellow stuff. I guess you'll just have to wait and see




Maybe infected. Damn.


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OfflineSgt. Pepper
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Re: Monotub taking forever to Pin [Re: microscopes]
    #18729413 - 08/19/13 07:49 PM (10 years, 5 months ago)

I see some brown spots, I'm new myself but if doesn't like 100% colonized. 100% colonization is the number one pinning trigger. Also the metabolizes can be cause you're colonizing at too high of a temperature. What are your temps like?


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OfflinePhosCap
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Re: Monotub taking forever to Pin [Re: microscopes]
    #18729670 - 08/19/13 08:40 PM (10 years, 5 months ago)

I say 3-5 days before you start seeing pins. I think I saw some knots in those pics


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Offlinemicroscopes
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Re: Monotub taking forever to Pin [Re: PhosCap]
    #18731056 - 08/20/13 06:27 AM (10 years, 5 months ago)

Jeez. Gonna be going on 9 days.

Misted and fanned, got humidity back up to 90%. Now there's just a layer of water on top.


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OfflinePussyFart
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Re: Monotub taking forever to Pin [Re: microscopes]
    #18731068 - 08/20/13 06:33 AM (10 years, 5 months ago)

There should be droplets of water, but no polling on the substrate.

Sometimes it just takes it's fuckin time.....I am playing the waiting game too with my PE.


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Offlinemicroscopes
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Re: Monotub taking forever to Pin [Re: PussyFart]
    #18731089 - 08/20/13 06:48 AM (10 years, 5 months ago)

Quote:

Notahacker420 said:
There should be droplets of water, but no polling on the substrate.

Sometimes it just takes it's fuckin time.....I am playing the waiting game too with my PE.




Killing me because I feel something must be wrong at this point. 9 days in fruiting condition without a single pin. Plus the yellowish brownish stuff


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InvisibleUhurungus
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Re: Monotub taking forever to Pin [Re: microscopes]
    #18731304 - 08/20/13 08:34 AM (10 years, 5 months ago)

i've got some PE that's been in fruiting for 11 days and nothing yet.  everything looks fine as far as i can tell.  i'll wait to start worrying until this weekend.

edit: day 12.  actually i just looked, i see pins have popped overnight


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Edited by Uhurungus (08/20/13 08:39 AM)


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Offlinemicroscopes
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Re: Monotub taking forever to Pin [Re: Uhurungus]
    #18733076 - 08/20/13 04:19 PM (10 years, 5 months ago)

More pictures

Anyone have any idea why it won't pin???



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Offlinemicroscopes
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Re: Monotub taking forever to Pin [Re: microscopes]
    #18733438 - 08/20/13 06:12 PM (10 years, 5 months ago)

Anyone? RR?


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Re: Monotub taking forever to Pin [Re: PussyFart]
    #18733665 - 08/20/13 06:54 PM (10 years, 5 months ago)

Quote:

psillyshroomer said:
Yes normal. Give it a few more days





Quote:

phosi said:
I say 3-5 days before you start seeing pins. I think I saw some knots in those pics





Quote:

Notahacker420 said:
Sometimes it just takes it's fuckin time.....I am playing the waiting game too with my PE.




nobody can give you a definitive answer because so many things play a role.

All you can do is make sure your FAE and RH are good and then wait. I've had stuff not fruit at all. Sometimes it's just genetics.

Just keep an eye and nostril on it.

I see some rhyzomorphic growth. But, the cottony appearance with metabolites along with slow pin time may indicate a contamination. The key word there is "may".

Do you have FAE holes near the top of the bin?

What was you pasteurization method?

Are you sure your spawn was 100% colonized?

How did you mix the spawn and sub?

Were your monotub holes taped over during sub colonization?

I know it's a lot of questions, just trying to get some possibilities together.


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OfflineBloodKil
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Re: Monotub taking forever to Pin [Re: microscopes]
    #18733675 - 08/20/13 06:55 PM (10 years, 5 months ago)

For fucks sake learn some patience...  bumping your posts after 2 hours is frowned upon and against the rules.

Besides that fact you already have a couple answers above... 

1 your sub is not fully colonized. You should not have put it into fruiting when you did.  (Patience is the key)

2. It's only been 7 days.  The earliest you will see pins is likely 7 days after fruiting if you put it in right when it hits 100% colonization...  (once again patience.)

So it seems to me the only problem you have for your tub issues and your posting issues is that your too impatient.  Have a drink, smoke a bowl, or just plain relax.


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Offlinemicroscopes
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Re: Monotub taking forever to Pin [Re: BloodKil]
    #18736807 - 08/21/13 10:38 AM (10 years, 5 months ago)

Quote:

BloodKil said:
For fucks sake learn some patience...  bumping your posts after 2 hours is frowned upon and against the rules.

Besides that fact you already have a couple answers above... 

1 your sub is not fully colonized. You should not have put it into fruiting when you did.  (Patience is the key)

2. It's only been 7 days.  The earliest you will see pins is likely 7 days after fruiting if you put it in right when it hits 100% colonization...  (once again patience.)

So it seems to me the only problem you have for your tub issues and your posting issues is that your too impatient.  Have a drink, smoke a bowl, or just plain relax.




For fucks sake, learn how to read. It's been 10 days now. Most people are harvesting their first flush right now and I don't even have hyphal knots. And you think I'm being impatient? WTF?


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OfflinePussyFart
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Re: Monotub taking forever to Pin [Re: microscopes]
    #18736860 - 08/21/13 10:48 AM (10 years, 5 months ago)

Quote:

microscopes said:
It's been 10 days now. Most Some people are harvesting their first flush right now and I don't even have hyphal knots. And you think I'm being impatient? WTF?



Yes.


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InvisibleFrankHorrigan
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Re: Monotub taking forever to Pin [Re: Stromrider]
    #18736883 - 08/21/13 10:52 AM (10 years, 5 months ago)

Quote:

psillyshroomer said:
That myc does not look normal to me. It is really fluffy and cottony looking not to mention the yellow stuff. I guess you'll just have to wait and see




You are correct.

I'm not sure why people miss these common indicators but let me spell it out...

OP you have a rampant bacterial contamination.

The uncolonized bits after 10 days, the thick, fluffy myc...might be the start of wet bubble in there as well.  Those "secretions" are going to get worse.

That thing is contam'd :frown:

Start new jars, check sterile procedures, cultures, etc.  Pasteurize properly.

Bacteria of this scale usually comes from either grain spawn or something like coffee grounds in the sub...since you used just coir and verm, I imagine you had a bad jar or three.


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InvisibleFrankHorrigan
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Re: Monotub taking forever to Pin [Re: FrankHorrigan]
    #18737071 - 08/21/13 11:45 AM (10 years, 5 months ago)

Found some of my old pics.  These are contam'd with what appears to be the same issue you are having:




The pooling water, the wet bubble-esque thick growth and elevated metabolite production...all of these are indicators of bacteria and a hot substrate.

It is not going to fruit :frown:


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You should take a look. :hehehe:


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OfflineStromriderM
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Re: Monotub taking forever to Pin [Re: FrankHorrigan]
    #18737802 - 08/21/13 02:29 PM (10 years, 5 months ago)

Yeah those look almost identical to what he's got


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Offlinemicroscopes
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Re: Monotub taking forever to Pin [Re: FrankHorrigan]
    #18738026 - 08/21/13 03:10 PM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
Found some of my old pics.  These are contam'd with what appears to be the same issue you are having:




The pooling water, the wet bubble-esque thick growth and elevated metabolite production...all of these are indicators of bacteria and a hot substrate.

It is not going to fruit :frown:




Ding, ding, ding - WE HAVE A WINNER!

Thank you Frank.

I wasn't being impatient. Something just wasn't right. I'll toss it out.

The jars looked perfect before using them, but I guess I just didn't see it. Too bad.


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Re: Monotub taking forever to Pin [Re: microscopes]
    #18738036 - 08/21/13 03:11 PM (10 years, 5 months ago)

What tek did you follow for pasteurizing your substrate?


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Re: Monotub taking forever to Pin [Re: microscopes]
    #18738042 - 08/21/13 03:12 PM (10 years, 5 months ago)

Pinning is triggered by a few things depending on species. Co2 levels, temperature, humidity. Make sure you are at >95% humidity, try loosening your poly.


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Re: Monotub taking forever to Pin [Re: cmmenke]
    #18738057 - 08/21/13 03:15 PM (10 years, 5 months ago)

Quote:

cmmenke said:
Pinning is triggered by a few things depending on species. Co2 levels, temperature, humidity. Make sure you are at >95% humidity, try loosening your poly.



Shoulda read the thread :P They already figured out the problem.


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OfflinePhosCap
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Re: Monotub taking forever to Pin [Re: d9c6]
    #18738109 - 08/21/13 03:25 PM (10 years, 5 months ago)

Quote:

d9c6 said:
Quote:

cmmenke said:
Pinning is triggered by a few things depending on species. Co2 levels, temperature, humidity. Make sure you are at >95% humidity, try loosening your poly.



Shoulda read the thread :P They already figured out the problem.




cmmenke, did you also mean to say FAE


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Re: Monotub taking forever to Pin [Re: PhosCap]
    #18738127 - 08/21/13 03:28 PM (10 years, 5 months ago)

Don't just throw it away...try burying it in your yard


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