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Offlinepsykonautix
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Registered: 07/21/13
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LC from Trusted Sterile Syringe
    #18725226 - 08/18/13 10:37 PM (10 years, 5 months ago)

If I make a LC using a spore syringe from a trusted vendor do I still have to worry about contams like I would from my own print?  Given that the LC medium is properly sterilized.


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InvisibleJuiceh
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Re: LC from Trusted Sterile Syringe [Re: psykonautix]
    #18725254 - 08/18/13 10:44 PM (10 years, 5 months ago)

Possibly. No vendor is perfect.

I've done it a few times successfully. I use 1/2 pint wbs jars to test my lc before g2g it to qt jars.


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Edited by Juiceh (08/18/13 10:46 PM)


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InvisibleFrankHorrigan
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Re: LC from Trusted Sterile Syringe [Re: Juiceh]
    #18725263 - 08/18/13 10:46 PM (10 years, 5 months ago)

There is practically no such thing as a 100% clean syringe.

So yes, suspect contam until it tests clean.


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Offlinepsykonautix
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Re: LC from Trusted Sterile Syringe [Re: FrankHorrigan]
    #18725345 - 08/18/13 11:08 PM (10 years, 5 months ago)

ok so if i use the syringe on one jar and it fruits correctly, can I then assume a LC from the same syringe will be clean?


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InvisiblePastywhyteMDiscord
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Re: LC from Trusted Sterile Syringe [Re: psykonautix]
    #18725370 - 08/18/13 11:17 PM (10 years, 5 months ago)

Quote:

psykonautix said:
ok so if i use the syringe on one jar and it fruits correctly, can I then assume a LC from the same syringe will be clean?




No, you might have lucked out on the first try.


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InvisibleFrankHorrigan
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Re: LC from Trusted Sterile Syringe [Re: Pastywhyte]
    #18725397 - 08/18/13 11:29 PM (10 years, 5 months ago)

I would say if it tests clean it is good.


If it contams later on, it is because the lc was compromised in the time between.


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InvisibleJuiceh
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Re: LC from Trusted Sterile Syringe [Re: FrankHorrigan]
    #18725766 - 08/19/13 01:50 AM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
I would say if it tests clean it is good.


If it contams later on, it is because the lc was compromised in the time between.



I think he's asking if the spore syringe would be clean if he lucks out knocking up an LC and uses that LC successfully to knock stuff up and eventually get fruits.


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Offlinepsykonautix
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Re: LC from Trusted Sterile Syringe [Re: Juiceh]
    #18726324 - 08/19/13 07:47 AM (10 years, 5 months ago)

So basically your saying there's no way to get a liquid culture that you KNOW is going to be free of contams.


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Offlinepsykonautix
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Re: LC from Trusted Sterile Syringe [Re: psykonautix]
    #18726333 - 08/19/13 07:50 AM (10 years, 5 months ago)

I don't want to knock up some 40-50 jars with LC without knowing if its good, and It's a little expensive to knock up that many jars with MS syringes.


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InvisiblePastywhyteMDiscord
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Re: LC from Trusted Sterile Syringe [Re: psykonautix]
    #18726753 - 08/19/13 09:47 AM (10 years, 5 months ago)

Agar and G2G is the way ya do it. Quick and clean :thumbup:


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OfflinebEelzeBosS
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Re: LC from Trusted Sterile Syringe [Re: Pastywhyte]
    #18726849 - 08/19/13 10:10 AM (10 years, 5 months ago)

I don't know if the sponsor I use is just that awesome or I'm extremely lucky but I've made 6 LC's from 6 sponsor spore syringes and all of them are clean. Doubt this comment was very helpful, just wanted to share my experience since everyone else will undoubtedly discourage you from doing it. I won't be making any more since I'm doing agar now, but I never had a problem...except trying to draw the LC into my syringe :grin:


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InvisiblebodhisattaMDiscordReddit
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Re: LC from Trusted Sterile Syringe [Re: bEelzeBosS]
    #18726866 - 08/19/13 10:18 AM (10 years, 5 months ago)

You could inoc the LC with 1CC and have it be fine and then the next 1CC from the MS syringe gets contams in a test jar just because by random chance a rogue spore left the syringe then rather than earlier or vice versa. Either way make the LC and test it and then use the rest of it to do the 20 more jars after it is confirmed viable. Otherwise take the MS syringe and expand it to 5-10 more syringes using PCed water in a self healing jar since most vendor syringes are concentrated enough to make between 60-120CC of inoculate in my experience. Or you could put a drop onto a agar plate or make a jar with BRF paste like a petri dish and then use the mycelium to make a LC but at that point you have a PC and can do much better for yourself anyway.


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InvisibleJuiceh
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Re: LC from Trusted Sterile Syringe [Re: psykonautix]
    #18726929 - 08/19/13 10:40 AM (10 years, 5 months ago)

Quote:

psykonautix said:
So basically your saying there's no way to get a liquid culture that you KNOW is going to be free of contams.



No, if the first syringe made with the LC from the jar is contamed then it is fucked. If the test jar grows fine from the first syringe then the LC is good.


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Offlinepsykonautix
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Re: LC from Trusted Sterile Syringe [Re: bodhisatta]
    #18727511 - 08/19/13 12:52 PM (10 years, 5 months ago)

But when you say test it you say it like i can just use a test strip lol.  That shit takes a month, and how long will a LC last.


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Offlinepsykonautix
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Re: LC from Trusted Sterile Syringe [Re: psykonautix]
    #18727543 - 08/19/13 12:59 PM (10 years, 5 months ago)

The whole reason I'm asking about this is because I used an LC last week that I made from a spore print I made six months ago to knock up 25 pint jars of rye berry, and the mycelium growing does not look rhizomorphic.  It looks more like cobweb to me, but it doesn't act like cobweb and all the jars look uniformly the same.

I'm trying to figure out if I am growing 25 jars of contam or do some psilocybe mycelium look different that others?


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InvisibleJuiceh
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Re: LC from Trusted Sterile Syringe [Re: psykonautix]
    #18727548 - 08/19/13 12:59 PM (10 years, 5 months ago)

Dude, 4CC of good LC into a WBS 1/2 pint jar will have it fully colonized in a week.


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How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


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InvisibleFrankHorrigan
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Re: LC from Trusted Sterile Syringe [Re: psykonautix]
    #18727553 - 08/19/13 01:00 PM (10 years, 5 months ago)

Quote:

psykonautix said:
But when you say test it you say it like i can just use a test strip lol.  That shit takes a month, and how long will a LC last.




No it doesn't.

Once you have healthy mycelial growth from your inoculation points, you know that it is clean.

Anything present in the LC will be visible within a week.

On agar, you only have to wait 2-3 days to check for growth.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlinepsykonautix
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Re: LC from Trusted Sterile Syringe [Re: FrankHorrigan]
    #18727573 - 08/19/13 01:04 PM (10 years, 5 months ago)

Thank you Frank that's what I was VERY unclear on.  When people kept telling me to test it I thought they meant I had to knock up one jar and wait till it fruited to see if it was ok. 

I put 5 cc's in each jar and they are colonizing fast as fuck, but the mycelium is not rhizomorphic. 

Any ideas on that?  Do some psilocybe strains not look rhizomorphic?  the LC was made from a print of psilocybe ecuadorian?


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OfflinebEelzeBosS
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Re: LC from Trusted Sterile Syringe [Re: psykonautix]
    #18727627 - 08/19/13 01:17 PM (10 years, 5 months ago)

Quote:

psykonautix said:

I put 5 cc's in each jar and they are colonizing fast as fuck, but the mycelium is not rhizomorphic. 

Any ideas on that?  Do some psilocybe strains not look rhizomorphic?  the LC was made from a print of psilocybe ecuadorian?



BRF jars? How far along? Ive had brf jars look kind of like little blobs of greyish white with no rhizo strands at all turn out fine. They were usually from MS though if I remember correctly. Can you post a pic?


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Offlinepsykonautix
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Re: LC from Trusted Sterile Syringe [Re: bEelzeBosS]
    #18728016 - 08/19/13 02:53 PM (10 years, 5 months ago)

I put 5cc's in the rye grain jars.  I'll post pix when i get home.


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Offlinepsykonautix
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Re: LC from Trusted Sterile Syringe [Re: psykonautix]
    #18729694 - 08/19/13 08:46 PM (10 years, 5 months ago)

Got home and since I shook all of them last night there's not that much to see yet will update tomorrow


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Offlinepsykonautix
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Re: LC from Trusted Sterile Syringe [Re: psykonautix]
    #18729737 - 08/19/13 08:54 PM (10 years, 5 months ago)

Here's a picture of the best one so far.  These were all shot up 4 days ago.



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