Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMythmaker
I'm a teapot


Registered: 05/21/13
Posts: 89
Last seen: 3 days, 17 hours
Black specks in LC: Contam or just ungerminated spores?
    #18724753 - 08/18/13 09:02 PM (10 years, 5 months ago)

Here's the rundown:

  • ~3.8% Karo/water solution
  • custom made lid (neoprene injection port, air filter tube packed full of polyfil and tyvek, all sealed with RTV)
  • pressure cooked for ~1hr
  • allowed to cool and knocked up with MS about 12 days ago







As the thread title says, I'm not sure if these tiiiiny black specks are contams or just ungerminated (cubensis) spores. They seem to be part of the big mycelial mass ... if they were contams, they'd be free-floating all around the jar wherever the nutrients are, right? And as you can see from the first pic, the karo water is totally clear except for the mycelia which kinda hovers near the bottom (but never "sits" on the bottom).

I just need some reassurance. Thanks. :p


--------------------


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 14 hours, 39 minutes
Trusted Cultivator
Re: Black specks in LC: Contam or just ungerminated spores? [Re: Mythmaker]
    #18728596 - 08/19/13 04:56 PM (10 years, 5 months ago)

I wouldn't use it. In fact I wouldn't make an LC from spores at all, especially if it's only karo/water. But if you do use it I suggest testing it first before inoculating a bunch of jars with right away.


--------------------


Extras: Filter Print Post Top
OfflineMythmaker
I'm a teapot


Registered: 05/21/13
Posts: 89
Last seen: 3 days, 17 hours
Re: Black specks in LC: Contam or just ungerminated spores? [Re: Kizzle]
    #18728648 - 08/19/13 05:06 PM (10 years, 5 months ago)

Quote:

Kizzle said:
I wouldn't use it. In fact I wouldn't make an LC from spores at all, especially if it's only karo/water.



Why not? I followed sterile procedure.

Quote:

Kizzle said:
But if you do use it I suggest testing it first before inoculating a bunch of jars with right away.



I know. I will. I'm gonna test it on 2 or 3 PF jars.


Also, I checked the LC again today and it appears that there are less black specks, and more mycelia. I guess this means that they were spores after all?


--------------------


Extras: Filter Print Post Top
OfflineJimmyhunter1000
That guy.
Male


Registered: 06/15/12
Posts: 777
Loc: Massachusetts, USA.
Last seen: 5 years, 9 months
Re: Black specks in LC: Contam or just ungerminated spores? [Re: Mythmaker]
    #18728783 - 08/19/13 05:36 PM (10 years, 5 months ago)

Quote:

Mythmaker said:
Quote:

Kizzle said:
I wouldn't use it. In fact I wouldn't make an LC from spores at all, especially if it's only karo/water.



Why not? I followed sterile procedure.

Quote:

Kizzle said:
But if you do use it I suggest testing it first before inoculating a bunch of jars with right away.



I know. I will. I'm gonna test it on 2 or 3 PF jars.


Also, I checked the LC again today and it appears that there are less black specks, and more mycelia. I guess this means that they were spores after all?




LC is notorious for harboring Contams. It's impossible to know if what you see in an LC is indeed Mushroom Myc or Mold myc. Testing out the LC in a jar is the only way to know for sure.


--------------------
There isn't a trail too long or too wide.


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 14 hours, 39 minutes
Trusted Cultivator
Re: Black specks in LC: Contam or just ungerminated spores? [Re: Mythmaker]
    #18728823 - 08/19/13 05:46 PM (10 years, 5 months ago)

Quote:

Why not? I followed sterile procedure.



Mainly from my own experiences trying them, all failures and I too was following sterile procedure. Spore prints are syringes aren't completely sterile. A syringe that produces a healthy jar may still produce a contaminated LC. It's a different environment so it has different effects on the growth of contaminants. You're far better off transferring sterile mycelium from an agar culture or even a grain jar to start an LC.

Also LCs colonize very slowly compared to other media. In the amount of time it would take for one LC to colonize completely from spores you could have a dozen LCs fully colonize by growing the mycelium out on agar or grain some first.


--------------------


Extras: Filter Print Post Top
OfflineMythmaker
I'm a teapot


Registered: 05/21/13
Posts: 89
Last seen: 3 days, 17 hours
Re: Black specks in LC: Contam or just ungerminated spores? [Re: Kizzle]
    #18729454 - 08/19/13 07:58 PM (10 years, 5 months ago)

I realize that nothing is ever 100% sterile, however just from what I've searched and read it seems like a majority of Shroomerites have made LC's from MS at some point and had a varying degree of success with them.

I don't have the materials for agar work yet, but I promise that I will learn it. I'll try a G2LC at some point too. But for now, I just have this one MSLC. I just wanted to stretch the last 2ml of spore solution i had. I'm still waiting for my next spore order.


--------------------


Extras: Filter Print Post Top
Offlineblthirteenre
Male User Gallery


Registered: 06/10/12
Posts: 430
Loc: New York
Last seen: 9 months, 17 days
Re: Black specks in LC: Contam or just ungerminated spores? [Re: Mythmaker]
    #18729541 - 08/19/13 08:17 PM (10 years, 5 months ago)

i have a question for those who dissuade people from using liquid culture... how exactly are people supposed to inoculate pf cakes?


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 14 hours, 39 minutes
Trusted Cultivator
Re: Black specks in LC: Contam or just ungerminated spores? [Re: blthirteenre]
    #18729804 - 08/19/13 09:09 PM (10 years, 5 months ago)

Quote:

how exactly are people supposed to inoculate pf cakes?



Spores
Really though, I'm not trying to dissuade anyone from using LCs. I just regret all the time I wasted dealing with contaminated jars after trying to make LCs directly from spores when I had virtually no problem making clean LCs from GLCs and agar cultures. Of course once you start working with agar, LCs seem kind of redundant anyway unless you're doing PF jars or grow bags.


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* black specks in colonizing jars vildechayea 2,979 9 02/06/12 05:12 AM
by CircleofFifths
* black specs,dots,(contam?) shroomme 1,993 4 11/12/01 08:15 PM
by Snobrdr311
* tiny black specks throughout mycillium MrMyco 1,217 1 08/26/03 08:41 AM
by Meph
* HELP!!! Black specks in the Karo water! thisone 2,983 9 08/28/03 03:11 PM
by wicish1
* contam or spores? mindship 843 2 11/08/01 09:07 AM
by Cub
* Black Ring Means Contam? WildLASER 1,506 5 08/01/02 11:54 AM
by WildLASER
* first grow, hope theres no contam... jerrygarcia 548 4 09/07/03 10:07 AM
by OJK
* Yikes! Help! (spore soloution) Anonymous 938 4 11/02/02 10:07 AM
by daussaulit

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,362 topic views. 35 members, 215 guests and 24 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.007 seconds on 14 queries.