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OfflineDustJuice
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help with contam
    #18724730 - 08/18/13 08:55 PM (10 years, 5 months ago)

Hi looking for some ideas on this contam. This is wheat (chicken feed) not all jars have contams but I seem to be running into this a lot. I suspect its from too much moisture before PCing. The grain was dried to standard (not sticking to spoon or leaving wetness on tp) but maybe there was too much moisture inside the grain that burst or seeped out from the climate when inside the jars. I have gotten this contam on GS in pp5 also. It makes the grain/GS become like molasses and develop a sweet hamy smell and stalls jar immediately. soaked 12-24hrs, PC'd 130mintues,with and without gypsium, noccd agar in SAB.I guess it could also be from the agar. Any ideas on prevention thx.







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OfflineDustJuice
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Re: help with contam [Re: DustJuice]
    #18726042 - 08/19/13 05:26 AM (10 years, 5 months ago)

found this in a search, I guess its helpful in that it could be a number of things  :um:

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Wet spot/Bacillus is [fortunately] an easy problem to overcome. It's encouraged by a few factors:

1. Incomplete PC cycle
I doubt that's the problem. 1 hour should really be enough. I don't work with rye, but I'd never go over an hour with WBS. You're going to 15 PSI, right?

2. High endospore-ridden grain
You've changed sources, which would have been a suggestion. Another option is to change the type of grain; I always failed with rye, but never tried to figure out the problem because [cheaper] WBS worked like a charm. Another step that can help, regardless of grain type, is to thoroughly rinse it before soaking/steeping.

3. Too much moisture
Some folks will tell you that steeping is better than soaking. What I'll tell you is that if you're having this big of a problem, you need to experiment to find what works for you. Run an entire load of jars as a 'test batch' with varying amounts of added water/steeping times from jar to jar. Label the jars and keep notes. This is going to require measuring the amount of water added [if any] so the best recipe can be reproduced easily. The details are up to you, but this is a very powerful way to get the water content right. . .you will likely have to pitch most of the test batch, but you will end up with a valuable 'recipe' that works *for you*, and can immediately run a full optimized load.

4. [rarely] jar to jar contamination
Often overlooked, but rarely the actual problem. If you have a wet/humid incubator, this is worth looking at. When a jar is PC'ing, it's possible that some of the liquid can boil over, essentially coating the outsides of the jar [or soaking the polyfill] with dilute grain water. After PC'ing, it's sterile, but is prime breeding grounds for bacteria [esp. in a wet incubator]. Bacillus is a motile bacterium, meaning it can swim up moist surfaces and possibly through filters/threads. If you think this is a possibility, replace the polyfill [a good idea anyway] and make sure to give the jars/lids a soapy scrub before re-use. Bleach/re-PC'ing will kill the organisms, but only scrubbing will remove the bacteria food source. Dry incubators/no incubators can also help.

One last thing; any time you're having problems of this type, it's a good idea to leave an uninoculated negative control jar [or two]. This is after you've figured out an optimal moisture content, of course. You seem confident that the inoculum is clean, but it's still a good idea. If the control ends up clean, it can be inoculated later.


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OfflineKizzle
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Re: help with contam [Re: DustJuice]
    #18728521 - 08/19/13 04:38 PM (10 years, 5 months ago)

Seems to me though wet-spot usually starts on the bottom where there's more moisture. The fact that it's started at the top might indicate your inoculant was the source or it's getting through your filter.


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OfflineDustJuice
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Re: help with contam [Re: Kizzle]
    #18730460 - 08/19/13 11:42 PM (10 years, 5 months ago)

thanks


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