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RossTmoney
TMoney


Registered: 07/24/13
Posts: 77
Last seen: 3 years, 8 months
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Co2 exchange in Jars
#18723982 - 08/18/13 06:06 PM (10 years, 5 months ago) |
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I have been reading about Co2 exchange during colonization and I think I may have mixed too many teks and made some mistakes. I read about poking the holes in the lids and filling them with silicon.   My jars are colonizing fine but some seem to be stalling out. I read this could be because lack of gas exchange. Is it unheard of to un-tighten the jar lids temperately to let some Co2 out?? Is that OK to do or do you suggest I just leave them as is. Out of about 50 Jars I have had about 10 reach 100% colonization. So I am somewhat confused about the importance. I am thinking about loosening the lids but I want to run it past the OMC first.. Any input would be helpful. Thanks.
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Mr. Wilson
experienced weiner rubber



Registered: 12/19/11
Posts: 1,977
Loc: Weinerville,
Last seen: 3 years, 1 month
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Re: Co2 exchange in Jars [Re: RossTmoney]
#18723999 - 08/18/13 06:11 PM (10 years, 5 months ago) |
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those lids look air tight to me=not good--IMO 50 jars man!!! fuck I hate that for ya
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Edited by Mr. Wilson (08/18/13 06:15 PM)
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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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Re: Co2 exchange in Jars [Re: RossTmoney]
#18724023 - 08/18/13 06:18 PM (10 years, 5 months ago) |
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2 holes with silicone would work well with a LC, but not much more than that. For GE you need a 9-10mm hole with either tyvek, a synthetic filter disc or polyfill. There's many lid teks out there.
On your second problem regarding how to establish GE, I have no idea. But those lids wont work.
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   The Corbett Report Open Source Intelligence News
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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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Re: Co2 exchange in Jars [Re: Pestile]
#18724044 - 08/18/13 06:23 PM (10 years, 5 months ago) |
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You can still use the lids, you just need to drill a hole in the middle and put a filter on/in.
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   The Corbett Report Open Source Intelligence News
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RossTmoney
TMoney


Registered: 07/24/13
Posts: 77
Last seen: 3 years, 8 months
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Re: Co2 exchange in Jars [Re: Pestile]
#18724092 - 08/18/13 06:33 PM (10 years, 5 months ago) |
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OK ya I know I messed up they ARE definitely air tight.. I would have to take the lids off to drill new holes anyways so that goes back to the question is it safe to remove /loosen lids mid-colonization??? What is surprising like I said I have had some strands Golden Teachers and Thai get to 100% colonized in 1/2 pint jars... So I just wanna know what to do. Part of me wants to loosen to lids or drill some holes and part of me is worried that will mess up shit or introduce contams (even though the verm layer helps protect for catams.)
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Mr. Wilson
experienced weiner rubber



Registered: 12/19/11
Posts: 1,977
Loc: Weinerville,
Last seen: 3 years, 1 month
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Re: Co2 exchange in Jars [Re: RossTmoney]
#18724572 - 08/18/13 08:20 PM (10 years, 5 months ago) |
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brother you went balls to the wall before you read what you should have read---take your time man--patience is key
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Co2 exchange in Jars [Re: RossTmoney]
#18724743 - 08/18/13 08:59 PM (10 years, 5 months ago) |
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If the top of the grains are fully colonized you can remove the lid, you should do this. The healthy mycelium at the top of the jar should prevent any contams. I have done this several times without problems. There are 5 jars on the shelf now that I have done this too, for different reasons that yours though and no contams. If they aren't fully colonized on top, you're in a tight spot cuz they need to breath but they may not be able to fight off contams. I would get a large plastic tote that is see through, and put those jars in there then unscrew the lids slightly, ever so slightly, just an 1/8th of a turn. Once all the jars lids are cracked a bit put the tote lid back on. The tote will have some amount of air for gas exchange but will also prevent wind currents from pushing contam spores under the lid. Once the top is colonized, remove the jars from tote and rest them on a shelf, then re-do the lids if desired. You could also build a SAB then crack the lids 1/8th of a turn, this would be even more fool-proof.
you should atleast wipe down the jars with alcohol before you attempt this, and sterilize the SAB.
As en extra precaution you could get a mini tote inside the SAB, one just big enough to contain a quart jar and fill it partially with Iso alcohol and dunk the jar in the IPA bath (using gloves). This would be more effective at getting alcohol into the small space under the lid ring. I would do this if I were in your position IPA is cheap and it might make the difference. If contam spores are just under the lid ring, and they probably are, you could contam the jar by untwisting it even partially. Then leave the jars in the tote until the top colonizes. I haven't tried this before however and I'm a little concerned that the heavy alcohol fumes might have an effect on the myc since they would be trapped in a tote.
If you really want to go the extra mile, dunk in IPA (inside a SAB) then put the jars inside a tyvek sleeve. Tie the sleeve shut, crack the lid 1/8th turn, then set it on a shelf and let it colonize. This should get you the fae without contams. Also, after the tyvek sleeve is closed, it might be wise to wait a few minutes (15 minutes?) for the alcohol to evaporate off the jar before cracking the lid, alcohol works to destroy contams only as it evaporates. I think RR said that.
Edited by invitro (08/18/13 09:14 PM)
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ziggity
Stranger



Registered: 07/05/13
Posts: 147
Last seen: 9 years, 10 months
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Re: Co2 exchange in Jars [Re: invitro] 1
#18725035 - 08/18/13 10:02 PM (10 years, 5 months ago) |
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You don't even need all those "high tech" lids in the first place... I literally just had a 100% success rate in open air, no SAB with simply a hole nailed in the lid and was using a MS syringe not live culture.
It's simple... You need just that one hole to inoculate them and create gas exchange and from there you just need to keep it in a contam free space. I did recently get a SAB but before I had it I would simply go into my kitchen and bleach wipe all counters and heavily spray lysol to neutralize any airborne contaminants. From there, you just make sure you keep the jars as safe as possible once they're pulled from the pressure cooker. I colonize them in simply a navy blue tote with shelving units in it that stay at a constant 75-77 degrees. The key is to not let anything touch or get above that hole and you'll be fine from there.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Co2 exchange in Jars [Re: ziggity]
#18725076 - 08/18/13 10:09 PM (10 years, 5 months ago) |
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Quote:
ziggity said: spray lysol to neutralize any airborne contaminants.
Lysol is a surface disinfectant that does not do anything against airborne spores.
You got lucky, which is awesome for you but no reason to recommend piss-poor sterile tek.
And the verm barrier on top of the cake is the contam barrier. So you are correct, no additional filter or self-healing ports is needed
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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My advice was based on the assumption you were using whole grain jars without a verm barrier. I suppose the same advice could apply to pftek jars though.
Edited by invitro (08/18/13 10:42 PM)
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Jackthetripper7



Registered: 05/14/12
Posts: 369
Loc: Down the rabbit hole
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Re: Co2 exchange in Jars [Re: invitro]
#18725296 - 08/18/13 10:53 PM (10 years, 5 months ago) |
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IME loosening jar lids is a good way to release built up co2 in airtight jars. I'd unscrew the lids about a quarter-turn. Not completely airtight but loose enough to let co2 leak out.
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RossTmoney
TMoney


Registered: 07/24/13
Posts: 77
Last seen: 3 years, 8 months
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Thank You all for the input ya I counted my jars I got 62 total with only 3 contams.. I was naive enough to buy a grow kit off Caligrowkits ad realized after the fact I spent $100 on a water heater and a air pump. Everything else could be bought separate for much less money. I tried to put a review on their site telling people this and they never posted it!! Out of my 62 jars I have on had the 3 contams and they were all the pre-made jars from caligrowkits. So I have learned a lot and do appreciate the input. P.S. what does IME stand for???
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Jackthetripper7



Registered: 05/14/12
Posts: 369
Loc: Down the rabbit hole
Last seen: 9 years, 1 month
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Re: Co2 exchange in Jars [Re: RossTmoney]
#18727007 - 08/19/13 11:02 AM (10 years, 5 months ago) |
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Learning to prep your own subs/grains is cheaper and its a must-know if you take this hobby seriously. IME stands for "in my experience"
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