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Offlineshroomyaxn
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Registered: 11/13/11
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LC LME & DEXTROSE
    #18723182 - 08/18/13 02:51 PM (10 years, 5 months ago)

I used dry LME and Dextrose; a tsp of each dry ingredient was added to 1 cup of recently boiled water.  I simmered the solution for 15-20 mins.  The hot solution was then filtered through double layered coffee filters twice, then added to a pint jar and pressure cooked for 20mins at 15psi. 
The lid of the pint jar has 3 small 1/8inch GE holes.  (the holes are barley an 8th of an inch because clear silicone coats the edges of each hole ) The inoculation hole is sealed with red high TEMP silicone.  A layer of tyvek is sandwiched between the RTV silicone, so the tyvek is essentially never pierced. 

The cooled jar was knocked up with 3ccs of Creeper MS.  The solution has been stirred 3 times a day using a stir bar. 
Im becoming NOT a fan of sugar based LC's of any kind because the viability/uncertainty of the solutions.  I have created some successful sugar based LC's in the past with liquid LME and light Karo.  My most recent attempt with the ingredients listed above did not seem to head in the right direction so i scraped those 2 pint jars and started over, I believe i mistakenly added too much dextrose and LME creating that seemed to have caused a murky swampy LC that looked contaminated. 
Here are some pics:

24 hours after MS inoc


Growth around sediment...not sure if this looks normal?


Pic of sediment from beneath pint jar


3 days after inoculation and 3min stir-bar stir, white "stuff" floating on top of LC


Day 3 just after a stir


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Invisible36fuckin5
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Loc: Diving into Mystical Territori...
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Re: LC LME & DEXTROSE [Re: shroomyaxn]
    #18723191 - 08/18/13 02:54 PM (10 years, 5 months ago)

Looks fine, but there's no way to tell till you test it.

Also, next time use scales and weigh it out to about 2-3% sugar/malt content.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offlineshroomyaxn
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Re: LC LME & DEXTROSE [Re: 36fuckin5]
    #18724962 - 08/18/13 09:47 PM (10 years, 5 months ago)

Okay will do.  So if 2% of LME per 1cup of water is 4.73ml then weight out 4.73grams?
Also should this LC eventually become less murky once the mycelium has really taken hold?
Thanks! :thumbup:


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InvisibleFrankHorrigan
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Re: LC LME & DEXTROSE [Re: shroomyaxn]
    #18725104 - 08/18/13 10:14 PM (10 years, 5 months ago)

I don't think it looks fine but again, no telling til you test it.

Best LC for cubes IMO: one gram of LME per 600mL of water, no additional sugars.


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You should take a look. :hehehe:


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Offlineshroomyaxn
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Re: LC LME & DEXTROSE [Re: FrankHorrigan]
    #18729298 - 08/19/13 07:29 PM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
I don't think it looks fine but again, no telling til you test it.

Best LC for cubes IMO: one gram of LME per 600mL of water, no additional sugars.




What are you seeing that makes you think its not fine? 
I too feel something isn't right, based upon my past experiences creating LC's :confused:

Here are updated pics:

Taken in room light just seconds after a 3minute full vortex stir. Before the strong stir I could see a large clump of myc through the murkiness.


Pic taken just after stir with LED flashlight up against pint jar.


Pic from beneath pint jar with LED flashlight shining on pint jar.  Sediment/myc/contam? recently floated to bottom after vortex stir.

Tomorrow i will create a quart jar LC with 600ml bottled water and just 1gram LME, no sugar added.


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Invisible36fuckin5
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Posts: 12,079
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Re: LC LME & DEXTROSE [Re: shroomyaxn]
    #18729920 - 08/19/13 09:37 PM (10 years, 5 months ago)

On second look, that seems bacterial, unless it's some kind of camera issue. It should not be cloudy at all, you should just have clouds of snot-looking myc in the bottom of your LC, or possibly floating on top, depending on how much you stir/shake.

I wouldn't use it. Good eye, Frank.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offlineshroomyaxn
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Registered: 11/13/11
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Re: LC LME & DEXTROSE [Re: 36fuckin5]
    #18743680 - 08/22/13 06:18 PM (10 years, 5 months ago)

That jar definitely lost the battle...the murkiness continued even a week after inoculation and the floating substance on the surface was not the white myc patties i was familiar with seeing. 

So I prepared a qt jar full of 600ml distilled water with 1 gram of LME.  A digital scale was used to measure out 1.0g of LME.  The gram was added to the heated water and stirred down for 10mins.  Chucks of LME powder dissipated within seconds of stirring.  I also skipped the filtering step through coffee filters this time around.  I also pressure cooked the LC for 30mins at 15psi.  Probably overkill for just 1qt of LC, but i needed to try a different approach.  The qt jar was inoculated with 1-2cc of CREEPER ms after a night of cooling off. 
Here are two pics just 36hours after inoculation.



Small growth hovering just above the bottom of the jar.  I can make out what seems to be a large spore in the center of the small floating mass. 
This was the last bit of creeper ms in the syringe so the spores looked plentiful before inoculation.

Update to come in one week.


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InvisibleLustyLocks
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Registered: 01/10/12
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Re: LC LME & DEXTROSE [Re: shroomyaxn]
    #18744269 - 08/22/13 08:29 PM (10 years, 5 months ago)

LC's with MS have always contaminated for me. I don't think one can really ever trust a MS syringe to be completely contam free.

When I make LC's, I like to use an agar wedge to inoculate. I have had success every time doing it that way. :thumbup:

Also, something else I like to do is place my dry ingredients in a double layered coffee filter and tie it off with a rubber band or something and put that in the boiling water until everything dissolves. That elimates the whole filtering process and makes things easier IMO.

I wish you luck on your LC journey.


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Offlineshroomyaxn
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Re: LC LME & DEXTROSE [Re: LustyLocks]
    #18780878 - 08/31/13 10:47 AM (10 years, 4 months ago)

Heres an update of the 1g LME with 600ml water LC: growth looks different than before.  Its stringier and whiter but this will of course need to be tested to be sure. 
While i was away i wasn't able to agitate the LC and my cabinet incubator reached temps in the high 80s...temps are back to normal and i am agitating daily. 
Pics.


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