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rew736
Your local stranger



Registered: 12/17/12
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Cracking agar?
#18720332 - 08/17/13 08:16 PM (10 years, 5 months ago) |
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I recently got my hands on a treasure coast syringe, and I put a couple drops on the center of my agar plate. A couple of days later the growth looked really bumpy. I looked through the plate from the bottom, and it appeared to be cracked. The agar is only cracked where there is myc/contam growth. Can someone explain this? Should I be worried?
-------------------- My trade list malicom said: "Banana's emit radiation. In fact banannas are the standard in detecting solar radiation. The radiation read from a bananna tells scientists how active the sun was during the growing season. Make sure to steer clear of bananas too." seekaytea said: "I need to stop spending all my money on plants and take up a cheaper hobby, like heroin." Wiccan_Seeker said: "Your knowledge on male-on-male rape and willingness to engage in fantasy regarding the matter is commendable." eminemvs.slimshady said: "I want to know if they are active and magic or not. And if they are not active, can i pick them before they are active and then they will become active or do they have to grow till they activate?"
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LiquidGlass
Glass Blower


Registered: 07/08/12
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Re: Cracking agar? [Re: rew736]
#18720370 - 08/17/13 08:32 PM (10 years, 5 months ago) |
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I am new to agar but it doesnt sound right. What type of agar was used? Did you open the petri dish in a SAB or by a HEPA?
Is it just cracked on the surface or all the way through? I had one with bactereial infection that cracked just on the surface where the infection was.
-------------------- Some art I've made Glass Art Gallery
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matsc
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Re: Cracking agar? [Re: rew736]
#18720482 - 08/17/13 08:55 PM (10 years, 5 months ago) |
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Ive seen this happen in my cultures occasionally, though I must admit im not entirely sure of the cause. Something to do with the growth habit of the fungi. If it grows and contracts it can pull apart the agar I suppose.
Its never been a problem for me, other than being a bit annoying.
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fastfred
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Re: Cracking agar? [Re: matsc]
#18721655 - 08/18/13 03:45 AM (10 years, 5 months ago) |
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It happens. Don't know if I agree that it's related to the growth, as that should be happening on the surface and shouldn't really be splitting agar.
It does happen though. My guess is that your agar is sticking to the sides of the dish a little more than normal. When it contracts normally it just pulls away from the sides. If it's stuck to the sides then it may split/crack.
What media are you using?
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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rew736
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Re: Cracking agar? [Re: fastfred]
#18722557 - 08/18/13 11:53 AM (10 years, 5 months ago) |
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Well it does not change anything, I am just curious. I am using MEA. I think it is the FDA BAM M93. http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm063900.htm
I used to use fastfred's (yours) recepies, but I couldn't find the recepie book... All I could find was a list of all your types of recepies. (DFA, PDA, MEA etc.)
Well, my theory is when you get either vigorous growth or lots of different contams, they suck the moisture out of the agar causing it to contract and eventually crack. That is why the cracks are under the growth. Does that even make sense?
-------------------- My trade list malicom said: "Banana's emit radiation. In fact banannas are the standard in detecting solar radiation. The radiation read from a bananna tells scientists how active the sun was during the growing season. Make sure to steer clear of bananas too." seekaytea said: "I need to stop spending all my money on plants and take up a cheaper hobby, like heroin." Wiccan_Seeker said: "Your knowledge on male-on-male rape and willingness to engage in fantasy regarding the matter is commendable." eminemvs.slimshady said: "I want to know if they are active and magic or not. And if they are not active, can i pick them before they are active and then they will become active or do they have to grow till they activate?"
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clueless
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Re: Cracking agar? [Re: rew736]
#18722788 - 08/18/13 12:59 PM (10 years, 5 months ago) |
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rew736
Your local stranger



Registered: 12/17/12
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Re: Cracking agar? [Re: clueless]
#18722806 - 08/18/13 01:07 PM (10 years, 5 months ago) |
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Perfect, gotta bookmark that!
-------------------- My trade list malicom said: "Banana's emit radiation. In fact banannas are the standard in detecting solar radiation. The radiation read from a bananna tells scientists how active the sun was during the growing season. Make sure to steer clear of bananas too." seekaytea said: "I need to stop spending all my money on plants and take up a cheaper hobby, like heroin." Wiccan_Seeker said: "Your knowledge on male-on-male rape and willingness to engage in fantasy regarding the matter is commendable." eminemvs.slimshady said: "I want to know if they are active and magic or not. And if they are not active, can i pick them before they are active and then they will become active or do they have to grow till they activate?"
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Cracking agar? [Re: rew736]
#18724414 - 08/18/13 07:37 PM (10 years, 5 months ago) |
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Quote:
rew736 said: Well, my theory is when you get either vigorous growth or lots of different contams, they suck the moisture out of the agar causing it to contract and eventually crack. That is why the cracks are under the growth. Does that even make sense?
Sure, that makes perfect sense. Unlike bacteria, myc actively transports nutrients and moisture. This could cause contraction of the agar. If it's not even then instead of pulling away from the sides it might easily cause cracks in the agar.
In any case, it's nothing to worry about. Varying the gel strength might cause or prevent this, but it's probably not worth really looking at.
-FF
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