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Offlinerockstaradam
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Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Is glovebox REALLY necessary? *DELETED*
    #18716444 - 08/16/13 09:14 PM (10 years, 5 months ago)

Post deleted by rockstaradam

Reason for deletion: Done



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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716467 - 08/16/13 09:21 PM (10 years, 5 months ago)

Simple open air innocs will work, its just not the smartest thing to do. But if you're in this hobby for a while and plan to go agar work, G2G transfers, cloning, etc you need to use a sab or hood.

Also you should skip the alcohol wipe on your syringe. Just flame red hot and inject. The first few drops from your syringe will quench the heat.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinerockstaradam
Stranger

Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: maddchef]
    #18716472 - 08/16/13 09:23 PM (10 years, 5 months ago)

I'm only using ms inoculations right now... I will still take the normal precautions of closing windows, turning ac off, long sleeves, pants, gloves, mask, shower, etc., because I like doing that ritual, but I HATE working in a small ass sab... Thanks


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Invisiblemaddchef
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Posts: 5,602
Loc: Your mom's vag
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716477 - 08/16/13 09:24 PM (10 years, 5 months ago)

If you're doing open air innocs there's no need for showers and all that jazz, just flame and inject.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinerockstaradam
Stranger

Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: maddchef]
    #18716493 - 08/16/13 09:29 PM (10 years, 5 months ago)

Word!! How long should I let jars cool after pc is off?


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Invisiblemaddchef
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Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716498 - 08/16/13 09:30 PM (10 years, 5 months ago)

Room temp/cool to the touch

But let me reiterate, I don't advocate such.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinerockstaradam
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Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: maddchef]
    #18716512 - 08/16/13 09:33 PM (10 years, 5 months ago)

Open air inoculation I assume you're speaking of?


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Invisiblemaddchef
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Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716521 - 08/16/13 09:35 PM (10 years, 5 months ago)

Yep


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinerockstaradam
Stranger

Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: maddchef]
    #18716593 - 08/16/13 09:55 PM (10 years, 5 months ago)

Alright. I'll just use my sab and do it the way I've been doing it


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InvisibleMagicman69
All About the Benjamins
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Registered: 05/29/13
Posts: 6,876
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716786 - 08/16/13 10:49 PM (10 years, 5 months ago)

Open air is fine for PF Tek.


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Offlinerockstaradam
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Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: Magicman69]
    #18716813 - 08/16/13 10:56 PM (10 years, 5 months ago)

It's wbs.


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OfflineSagescruffy
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Registered: 10/30/09
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Loc: PNW
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Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716859 - 08/16/13 11:06 PM (10 years, 5 months ago)

Flaming the needle and then wiping with alcohol isn't good technique. When you flame the needle it is sterilized, but wiping with alcohol can actually reintroduce contaminates on your previously sterilized needle.


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Offlinerockstaradam
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Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: Sagescruffy]
    #18716877 - 08/16/13 11:09 PM (10 years, 5 months ago)

I beg to differ, agar ( I'm sure you know who that is ) uses alcohol only open air inoculation technique and has great success


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InvisibleChastity Psilocybe
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Registered: 09/06/12
Posts: 646
Loc: ON, Canada Flag
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716899 - 08/16/13 11:12 PM (10 years, 5 months ago)

My husband and I use open air inoculation.  We just spray lysol ALL OVER the room, put on our mask and gloves, then go in to inoculation.


--------------------
"Today you are you, that is truer than true.  There is no one alive that is youer than you" ~Dr. Seuss


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OfflineSagescruffy
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Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716910 - 08/16/13 11:13 PM (10 years, 5 months ago)

I don't know who that is and I'm way too lazy to search him up. Some people get away with things and some people don't. I'm just saying that when a needle is flamed, the needle is sterilized and wiping the needle afterward with an alcohol wipe defeats the purpose of flame sterilizing the needle.


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Offlinerockstaradam
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Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: Sagescruffy]
    #18716933 - 08/16/13 11:17 PM (10 years, 5 months ago)

I see where you're coming from and appreciate your input. That's just the way many people ( from what I've seen ) and I have done it for the longest time


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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
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Re: Is glovebox REALLY necessary? [Re: Sagescruffy]
    #18717045 - 08/16/13 11:37 PM (10 years, 5 months ago)

Quote:

Sagescruffy said:
I don't know who that is




This makes me sad


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18717592 - 08/17/13 03:12 AM (10 years, 5 months ago)

Quote:

rockstaradam said:
I see where you're coming from and appreciate your input. That's just the way many people ( from what I've seen ) and I have done it for the longest time




Quote:

Notahacker420 said:
Wiping alcohol on a flame sterilized needle is like wiping shit on your face after a shower.

Quote:

RR

Wiping with alcohol between jars is a good way to introduce mold spores into each jar, always flame sterilize between jars.

How are people who have been here this long still giving shit advice?




http://www.shroomery.org/forums/showflat.php/Number/17737262#17737262


--------------------


The Corbett Report
Open Source Intelligence News



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OfflineCMOS
Whats next?


Registered: 01/08/12
Posts: 833
Last seen: 9 years, 8 months
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18717609 - 08/17/13 03:26 AM (10 years, 5 months ago)

Quote:

uses alcohol only open air inoculation technique and has great success




I'm sure he was working in much better conditions too, most of us are working in our dirty ass living spaces (also I'm sure he has alot more experience on which corners can be cut).

Open air inoculations will work with certain inoculation techniques in certain environments, but for someone without experience its best to work in a $7.00 still air box for best success.  I've gotten away with open air G2Gs in the past (as an experiment), but why risk it?


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-------------------------------------------------

-------------------------------------------------


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Invisiblebudmanman
OTD Masterbater
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Registered: 02/07/07
Posts: 17,975
Loc: PNW
Re: Is glovebox REALLY necessary? [Re: CMOS]
    #18717636 - 08/17/13 03:36 AM (10 years, 5 months ago)

I did not use a glove box for the longest time but now that I do i thing its pretty nice.


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.


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Offlinepsilocybebonsai
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Registered: 09/17/05
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Re: Is glovebox REALLY necessary? [Re: CMOS]
    #18717649 - 08/17/13 03:42 AM (10 years, 5 months ago)

when you think about open air and SAB its really *almost* the same thing. in an SAB you're reducing the volume of space that can potentially contaminate your project.

i'd listen to everyone else... but just to throw my mix into the bowl, i treat my bathroom as a SAB. I put all my items in there (i use a tv-tray and a drumming throne as my main work area) and spray with lysol, close the bathroom door and as i'm doing it (slowly) i keep spraying as the gap in the door gets smaller. i make sure to get all the area i'll be using, from down low to up high.

i open/close doors very slowly, and i use excess amounts of alcohol on my hands/gloves and on my jars. if this is still considered open air (which of course it is) then its as close to jumping inside your SAB as it can get. I have had zero contamination in the past 20 jars/agar i've done.

i hope this helps you, as well as the input others have given.


--------------------
My small photo collection here
My smaller grow log here
Some tips for taking photos of your progress when seeking help here


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OfflineRogerRabbitM
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Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18717657 - 08/17/13 03:45 AM (10 years, 5 months ago)

Quote:

Is glovebox REALLY necessary?




No, you can do open air and have a higher fail rate.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinetlien
Male

Registered: 10/07/12
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Last seen: 9 years, 3 months
Re: Is glovebox REALLY necessary? [Re: RogerRabbit]
    #18717697 - 08/17/13 04:03 AM (10 years, 5 months ago)

I've only grown two batches of jars, and I live in a colder climate (less microbiology), but I had no contamination problems whatsoever (well, one glass, but I think that was caused by the mycelium refusing to continue spreading, for some reason), and I did not use a glove box or flow hood or anything.

I washed the glasses and the tub with soap.

I washed my hands with soap, and after they were dry, with alcohol. Always, before touching anything.

I put anything that I needed to store in a bag into a FRESH bag, one that has never been used, and I used flame sterilization on the syringe before every glass.

Results may vary, some seem to have infinite problems using this approach, some have none.


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Offlinerockstaradam
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Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: RogerRabbit]
    #18718020 - 08/17/13 07:49 AM (10 years, 5 months ago)

Quote:

RogerRabbit said:
Quote:

Is glovebox REALLY necessary?




No, you can do open air and have a higher fail rate.
RR



Alright RR hahaha


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