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Offlinerockstaradam
Stranger

Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Is glovebox REALLY necessary? *DELETED*
    #18716444 - 08/16/13 09:14 PM (10 years, 5 months ago)

Post deleted by rockstaradam

Reason for deletion: Done



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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716467 - 08/16/13 09:21 PM (10 years, 5 months ago)

Simple open air innocs will work, its just not the smartest thing to do. But if you're in this hobby for a while and plan to go agar work, G2G transfers, cloning, etc you need to use a sab or hood.

Also you should skip the alcohol wipe on your syringe. Just flame red hot and inject. The first few drops from your syringe will quench the heat.


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In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinerockstaradam
Stranger

Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: maddchef]
    #18716472 - 08/16/13 09:23 PM (10 years, 5 months ago)

I'm only using ms inoculations right now... I will still take the normal precautions of closing windows, turning ac off, long sleeves, pants, gloves, mask, shower, etc., because I like doing that ritual, but I HATE working in a small ass sab... Thanks


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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716477 - 08/16/13 09:24 PM (10 years, 5 months ago)

If you're doing open air innocs there's no need for showers and all that jazz, just flame and inject.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinerockstaradam
Stranger

Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: maddchef]
    #18716493 - 08/16/13 09:29 PM (10 years, 5 months ago)

Word!! How long should I let jars cool after pc is off?


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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716498 - 08/16/13 09:30 PM (10 years, 5 months ago)

Room temp/cool to the touch

But let me reiterate, I don't advocate such.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinerockstaradam
Stranger

Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: maddchef]
    #18716512 - 08/16/13 09:33 PM (10 years, 5 months ago)

Open air inoculation I assume you're speaking of?


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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716521 - 08/16/13 09:35 PM (10 years, 5 months ago)

Yep


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinerockstaradam
Stranger

Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: maddchef]
    #18716593 - 08/16/13 09:55 PM (10 years, 5 months ago)

Alright. I'll just use my sab and do it the way I've been doing it


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InvisibleMagicman69
All About the Benjamins
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Registered: 05/29/13
Posts: 6,876
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716786 - 08/16/13 10:49 PM (10 years, 5 months ago)

Open air is fine for PF Tek.


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Offlinerockstaradam
Stranger

Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: Magicman69]
    #18716813 - 08/16/13 10:56 PM (10 years, 5 months ago)

It's wbs.


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OfflineSagescruffy
CH
Male


Registered: 10/30/09
Posts: 2,011
Loc: PNW
Last seen: 6 months, 12 days
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716859 - 08/16/13 11:06 PM (10 years, 5 months ago)

Flaming the needle and then wiping with alcohol isn't good technique. When you flame the needle it is sterilized, but wiping with alcohol can actually reintroduce contaminates on your previously sterilized needle.


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Offlinerockstaradam
Stranger

Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: Sagescruffy]
    #18716877 - 08/16/13 11:09 PM (10 years, 5 months ago)

I beg to differ, agar ( I'm sure you know who that is ) uses alcohol only open air inoculation technique and has great success


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InvisibleChastity Psilocybe
Sylus' Apprentice
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Registered: 09/06/12
Posts: 646
Loc: ON, Canada Flag
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716899 - 08/16/13 11:12 PM (10 years, 5 months ago)

My husband and I use open air inoculation.  We just spray lysol ALL OVER the room, put on our mask and gloves, then go in to inoculation.


--------------------
"Today you are you, that is truer than true.  There is no one alive that is youer than you" ~Dr. Seuss


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OfflineSagescruffy
CH
Male


Registered: 10/30/09
Posts: 2,011
Loc: PNW
Last seen: 6 months, 12 days
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18716910 - 08/16/13 11:13 PM (10 years, 5 months ago)

I don't know who that is and I'm way too lazy to search him up. Some people get away with things and some people don't. I'm just saying that when a needle is flamed, the needle is sterilized and wiping the needle afterward with an alcohol wipe defeats the purpose of flame sterilizing the needle.


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Offlinerockstaradam
Stranger

Registered: 08/13/12
Posts: 382
Last seen: 2 months, 22 days
Re: Is glovebox REALLY necessary? [Re: Sagescruffy]
    #18716933 - 08/16/13 11:17 PM (10 years, 5 months ago)

I see where you're coming from and appreciate your input. That's just the way many people ( from what I've seen ) and I have done it for the longest time


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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Is glovebox REALLY necessary? [Re: Sagescruffy]
    #18717045 - 08/16/13 11:37 PM (10 years, 5 months ago)

Quote:

Sagescruffy said:
I don't know who that is




This makes me sad


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18717592 - 08/17/13 03:12 AM (10 years, 5 months ago)

Quote:

rockstaradam said:
I see where you're coming from and appreciate your input. That's just the way many people ( from what I've seen ) and I have done it for the longest time




Quote:

Notahacker420 said:
Wiping alcohol on a flame sterilized needle is like wiping shit on your face after a shower.

Quote:

RR

Wiping with alcohol between jars is a good way to introduce mold spores into each jar, always flame sterilize between jars.

How are people who have been here this long still giving shit advice?




http://www.shroomery.org/forums/showflat.php/Number/17737262#17737262


--------------------


The Corbett Report
Open Source Intelligence News



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OfflineCMOS
Whats next?


Registered: 01/08/12
Posts: 833
Last seen: 9 years, 8 months
Re: Is glovebox REALLY necessary? [Re: rockstaradam]
    #18717609 - 08/17/13 03:26 AM (10 years, 5 months ago)

Quote:

uses alcohol only open air inoculation technique and has great success




I'm sure he was working in much better conditions too, most of us are working in our dirty ass living spaces (also I'm sure he has alot more experience on which corners can be cut).

Open air inoculations will work with certain inoculation techniques in certain environments, but for someone without experience its best to work in a $7.00 still air box for best success.  I've gotten away with open air G2Gs in the past (as an experiment), but why risk it?


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-------------------------------------------------

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Invisiblebudmanman
OTD Masterbater
Male


Registered: 02/07/07
Posts: 17,975
Loc: PNW
Re: Is glovebox REALLY necessary? [Re: CMOS]
    #18717636 - 08/17/13 03:36 AM (10 years, 5 months ago)

I did not use a glove box for the longest time but now that I do i thing its pretty nice.


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.


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