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OfflineLoveLightPeace
Male

Registered: 08/26/11
Posts: 286
Last seen: 5 years, 1 day
Question about isolating sectors
    #18713469 - 08/16/13 07:51 AM (10 years, 6 months ago)

I have two Qs:

1-How big a chunk does one need to cut off from the edge of the mycelial mass in a petri dish (a multi strained one) to ensure that one is cutting a single strain of that multi strained mycelium? Im thinking in my head that if one cuts a big enough chunk even if its from the edge, one might unintentionally cut a multi strained sector!?

2-ive heard that if one is isolating sectors one should not allow the mycelium to grow so much as to touch the sides of the petri dish. Is this true?


--------------------
I am so small I can barely be seen.
How can this great love be inside me?

Look at your eyes. They are small,
but they see enormous things.

(Rumi)


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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Question about isolating sectors [Re: LoveLightPeace]
    #18713487 - 08/16/13 07:59 AM (10 years, 6 months ago)

If you can't tell which parts of the mycelium are individual sectors, you need to continue transferring blind.

When it is an isolate or close to it, you will know.

IME cubes don't start to show sectoring for at least 4 or 5 transfers. It also helps to change agar media on them to encourage strains to "run."

They will be pretty hard to tell apart with your first few transfers. Just take small, triangular pieces from the leading edge of the fastest growing healthy mycelium until you either have sectoring or uniform growth.

In the future, it's much easier and more effective to clone healthy, fast growing fruits from multispore grows than isolating sectors from MS.

You will know you have a good fruiter 9 times out of 10.

The edges of the petri are where your contams will fall if the lid or wrap is compromised.  I never let me cultures grow out to the edge period.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineLoveLightPeace
Male

Registered: 08/26/11
Posts: 286
Last seen: 5 years, 1 day
Re: Question about isolating sectors [Re: FrankHorrigan]
    #18715245 - 08/16/13 03:53 PM (10 years, 6 months ago)

thnx:)


--------------------
I am so small I can barely be seen.
How can this great love be inside me?

Look at your eyes. They are small,
but they see enormous things.

(Rumi)


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