|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
psykonautix
Stranger


Registered: 07/21/13
Posts: 187
Last seen: 9 years, 7 months
|
Re: flow hood question [Re: Pestile]
#18715741 - 08/16/13 05:53 PM (10 years, 5 months ago) |
|
|
I read it and just didn't get the fact about the turbulent air. That makes complete sense now thank you. I'm thinking im just gonna make one of these until I can afford the right filter and fan.
-------------------- Everything I say or post is a work of fiction and should be considered a lie.
|
Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
|
|
I would probably stick with a SAB. Just sayin'.
--------------------
   The Corbett Report Open Source Intelligence News
|
psykonautix
Stranger


Registered: 07/21/13
Posts: 187
Last seen: 9 years, 7 months
|
Re: flow hood question [Re: Pestile]
#18715779 - 08/16/13 06:01 PM (10 years, 5 months ago) |
|
|
SAB - "sealed air box" ???
-------------------- Everything I say or post is a work of fiction and should be considered a lie.
|
36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
|
|
Still air box.
I much prefer a schmuvbox. Don't hook it into a plastic box. Use the bag.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
|
psykonautix
Stranger


Registered: 07/21/13
Posts: 187
Last seen: 9 years, 7 months
|
Re: flow hood question [Re: 36fuckin5]
#18715785 - 08/16/13 06:03 PM (10 years, 5 months ago) |
|
|
gotcha, that one looks like such an easy design. I'm just gonna have to get a big one cuz I want to be able to do 20 jars at a time.
-------------------- Everything I say or post is a work of fiction and should be considered a lie.
|
36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
|
|
You're not gonna feasibly do 20 jars at a time without a flowhood. Sorry.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
|
psykonautix
Stranger


Registered: 07/21/13
Posts: 187
Last seen: 9 years, 7 months
|
Re: flow hood question [Re: 36fuckin5]
#18715944 - 08/16/13 06:45 PM (10 years, 5 months ago) |
|
|
they make some pretty big storage containers for cattle down here. hook it up to a shopvac
-------------------- Everything I say or post is a work of fiction and should be considered a lie.
|
36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
|
|
Quote:
psykonautix said: they make some pretty big storage containers for cattle down here. hook it up to a shopvac
No. No shop vac. SAB or flowhood.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
|
psykonautix
Stranger


Registered: 07/21/13
Posts: 187
Last seen: 9 years, 7 months
|
Re: flow hood question [Re: 36fuckin5]
#18717140 - 08/16/13 11:55 PM (10 years, 5 months ago) |
|
|
i found a 100 qt storage container like the one above that i'll be using tomorrow
-------------------- Everything I say or post is a work of fiction and should be considered a lie.
|
36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
|
|
Yeah, you're gonna be lucky to fit 10 jars in that with room to work. That's just the way it is.
Like I said, search up the Schmuvbox. People will put it down all day, but I've sworn by it for the last 4 years.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
|
CMOS
Whats next?


Registered: 01/08/12
Posts: 833
Last seen: 9 years, 8 months
|
|
Quote:
i found a 100 qt storage container like the one above that i'll be using tomorrow
I would just use it as a still airbox if you don't have a proper flowhood. No attached gloves or force filtered air, just two holes for your arms with some plastic sheets in front for when your not working. Any mold/bacteria will settle down and stick to the sides of your workspace.
I do my agar work inside a much smaller one (66 qt maybe). Its kind of a pain cause the ceiling isn't very high and I tend to hit the top when pouring plates. I usually can end up fitting about six quart jars plus a master for g2g comfortably. Which for my single AA921, I can get one load (10 qt jars) done in two SAB sessions.
-------------------- -------------------------------------------------
-------------------------------------------------
|
psykonautix
Stranger


Registered: 07/21/13
Posts: 187
Last seen: 9 years, 7 months
|
Re: flow hood question [Re: CMOS]
#18718328 - 08/17/13 10:00 AM (10 years, 5 months ago) |
|
|

Made this last night gonna start with a new spore print and new jars.
How long do you let it sit before working, and I know I can't use lysol because I'm using a butane lighter, so what else works?? bleach and water...
-------------------- Everything I say or post is a work of fiction and should be considered a lie.
|
Nakor420
Fun Guy



Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 9 years, 11 months
|
|
It's a still air box, not a sterile air box...
|
psykonautix
Stranger


Registered: 07/21/13
Posts: 187
Last seen: 9 years, 7 months
|
Re: flow hood question [Re: Nakor420]
#18718486 - 08/17/13 11:15 AM (10 years, 5 months ago) |
|
|
and why is that???
-------------------- Everything I say or post is a work of fiction and should be considered a lie.
|
Nakor420
Fun Guy



Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 9 years, 11 months
|
|
The idea is to moisten the inside and let contaminates settle and stick to the sides over time, then you do your work...sterilizing doesn't hurt, but it's not the main point of a SAB.
|
psykonautix
Stranger


Registered: 07/21/13
Posts: 187
Last seen: 9 years, 7 months
|
Re: flow hood question [Re: Nakor420]
#18718497 - 08/17/13 11:20 AM (10 years, 5 months ago) |
|
|
I get that, I just sprayed the inside down with lysol and everything in it. I'm gonna let it sit for a couple hours and come back and to the print transfer to LC.
-------------------- Everything I say or post is a work of fiction and should be considered a lie.
|
Nakor420
Fun Guy



Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 9 years, 11 months
|
|
Sounds like a good plan.
|
36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
|
Re: flow hood question [Re: Nakor420]
#18719376 - 08/17/13 03:36 PM (10 years, 5 months ago) |
|
|
Remove the gloves. They'll pull in dirty air from around your imperfect seal. Just 2 holes in a tub is all you need.
Also skip the Lysol. Bleach water or soapy water will work. Leave the box a little wet. Give it 10-15 minutes and go to work.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
|
psykonautix
Stranger


Registered: 07/21/13
Posts: 187
Last seen: 9 years, 7 months
|
|
It's sealed enough that I couldn't pull it off the table with the fan on.
-------------------- Everything I say or post is a work of fiction and should be considered a lie.
|
psykonautix
Stranger


Registered: 07/21/13
Posts: 187
Last seen: 9 years, 7 months
|
|
And I don't have any tyvek sleeves at the moment.
-------------------- Everything I say or post is a work of fiction and should be considered a lie.
|
|