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Invisiblemaddchef
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So what happens if.....
    #18714102 - 08/16/13 11:12 AM (10 years, 5 months ago)

So we all know you can make wbs "flour" cakes and BRF cakes and such.

But what happens if you pulverize a colonized jar or wbs or rye or any other grain, does it just contam all to hell seeing as the middle of the kernels isn't colonized?

Never tried or gave it much thought.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblebodhisattaMDiscordReddit
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Re: So what happens if..... [Re: maddchef]
    #21997357 - 07/25/15 09:26 PM (8 years, 6 months ago)

:derfase:
no one is safe tonight


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Invisiblemaddchef
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Re: So what happens if..... [Re: bodhisatta]
    #21997374 - 07/25/15 09:31 PM (8 years, 6 months ago)

Good score. Looks like I was all about slurries back then just never did anything with it.

From now on imma reply to my own posts just to be safe


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMudaFuka
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Re: So what happens if..... [Re: maddchef]
    #21997406 - 07/25/15 09:37 PM (8 years, 6 months ago)

Just in case anyone cares. It can be done but works best with smaller grains like millet and should not be blended too much. The un-colonised centers of the grain are a contam risk. It has been pulled off but when I tried it with colonised oats my substrate turned green shortly after full colonisation.


--------------------
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Re: So what happens if..... [Re: MudaFuka]
    #21997468 - 07/25/15 09:53 PM (8 years, 6 months ago)

:lol:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineMachiavelliavore
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Re: So what happens if..... [Re: MudaFuka]
    #21997947 - 07/26/15 12:20 AM (8 years, 6 months ago)

Ever tried a grass seed slury?  I'm about to give it a shot.  Seems like low starch content, lots of sheath and mycellium between grains, probably a good choice.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Invisiblemaddchef
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Re: So what happens if..... [Re: Machiavelliavore]
    #21997976 - 07/26/15 12:25 AM (8 years, 6 months ago)

I fail to grasp why it matters what type of grain is used. Likely the inside isn't colonized and is vulnerable.

This doesn't matter if slurry is made and distributed in a sterile manner though.

Cept maybe some bacteria issues.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinekiksen
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Re: So what happens if..... [Re: maddchef]
    #21998141 - 07/26/15 01:01 AM (8 years, 6 months ago)

Why would anybody do that?


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InvisibleMudaFuka
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Re: So what happens if..... [Re: maddchef]
    #21998628 - 07/26/15 07:10 AM (8 years, 6 months ago)

The grain matters because large grains like oats will have a lot higher ratio of un-colonised inner grain to mycelium. A smaller grain like RGS will have much less uncolonised grain matter and more mycelium. It's still a risk but with smaller grain there is a better chance that the cube mycelium will fully colonis the substrate before any contaminants have time to germinate. If kept sterile I don't see it being any more risky than G2G or LC.

@kiksen the main reason to slurry grain is speed. A substrate inoculated with slurry can reach 100% colonisation in just a couple of days.


--------------------
AMU
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InvisibleBuck513
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Re: So what happens if..... [Re: maddchef]
    #21998695 - 07/26/15 07:32 AM (8 years, 6 months ago)

Search function:smilingpuppy:


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Fail to plan and you plan to fail.

Enter the Ban Lottery


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Invisiblefilthyknees
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Re: So what happens if..... [Re: Buck513]
    #21999082 - 07/26/15 09:46 AM (8 years, 6 months ago)

I take a half quart of sterilized water, g2g in half a quart of spawn with blender/band assembly on top. Blend and expand. I tried this once and when I expanded it right away the results where 100%. I used WBS and blended about 10 seconds.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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Re: So what happens if..... [Re: filthyknees]
    #21999398 - 07/26/15 11:02 AM (8 years, 6 months ago)

Would be cool if pasty could run comparement trial(s):stoned:


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RETARDS!  I'LL IGNORE ALL OF YOUR ADVICE!  BUT TELL ME WHAT TO DO STILL!


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InvisibleMudaFuka
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Re: So what happens if..... [Re: Galba Cubensis]
    #21999425 - 07/26/15 11:05 AM (8 years, 6 months ago)

Comparemenent? Why Past? He doesn't use slurry. Eat is the slurry specialist around here.


--------------------
AMU
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OfflineGalba Cubensis
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Re: So what happens if..... [Re: MudaFuka]
    #21999476 - 07/26/15 11:14 AM (8 years, 6 months ago)

Or muda. Havent "met" eat. Someone should do it:crazy:


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RETARDS!  I'LL IGNORE ALL OF YOUR ADVICE!  BUT TELL ME WHAT TO DO STILL!


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Invisiblemaddchef
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Re: So what happens if..... [Re: Galba Cubensis]
    #21999569 - 07/26/15 11:33 AM (8 years, 6 months ago)

Compartment?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineGalba Cubensis
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Re: So what happens if..... [Re: maddchef]
    #21999733 - 07/26/15 12:11 PM (8 years, 6 months ago)

Bait taking comparison:
1day, pro taking, munched
2day, nibbled
3day->you guys just salty, wont even insult me:sad:


--------------------
RETARDS!  I'LL IGNORE ALL OF YOUR ADVICE!  BUT TELL ME WHAT TO DO STILL!


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InvisibleMudaFuka
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Re: So what happens if..... [Re: Galba Cubensis]
    #21999741 - 07/26/15 12:14 PM (8 years, 6 months ago)

:pleasetellmemore:


--------------------
AMU
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OfflineGalba Cubensis
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Re: So what happens if..... [Re: MudaFuka]
    #21999785 - 07/26/15 12:25 PM (8 years, 6 months ago)

Is it LC shes using?:eek:


--------------------
RETARDS!  I'LL IGNORE ALL OF YOUR ADVICE!  BUT TELL ME WHAT TO DO STILL!


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Invisiblemaddchef
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Re: So what happens if..... [Re: Galba Cubensis]
    #21999815 - 07/26/15 12:34 PM (8 years, 6 months ago)

Galba I will tell you but once, you're trolling the wrong thread.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineGalba Cubensis
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Re: So what happens if..... [Re: maddchef]
    #21999841 - 07/26/15 12:41 PM (8 years, 6 months ago)

Who said Im trolling? Id love for someone to do a chomparemenent, true story. Same with the UV comment in some other smoke thread.


--------------------
RETARDS!  I'LL IGNORE ALL OF YOUR ADVICE!  BUT TELL ME WHAT TO DO STILL!


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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: Galba Cubensis]
    #22000112 - 07/26/15 02:03 PM (8 years, 6 months ago)

A comparison of what? Yields will be similar,  eat proved that. The difference is in speed of spawn run. You achieve full colonization in 2-3 days. Eat proved it. It carries a slightly higher risk of contam. Not sure what else is needed :shrug:


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OfflineGalba Cubensis
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Re: So what happens if..... [Re: Pastywhyte]
    #22000288 - 07/26/15 02:50 PM (8 years, 6 months ago)

Didnt know eat proved anything, there are some who say there are 10k pages, so there is still alot I havent "sorted through" and prob never will. Shouldnt you pros be doing this all the time then? Since, if I remember correctly, its how rarely you get contams that makes one pro?


--------------------
RETARDS!  I'LL IGNORE ALL OF YOUR ADVICE!  BUT TELL ME WHAT TO DO STILL!


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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: Galba Cubensis]
    #22000310 - 07/26/15 02:56 PM (8 years, 6 months ago)

I grow the way I grow. Eat grows his own way. Both are good. I change things up and try stuff but I have been busy doing my own thing. Maybe later I will try slurry more in depth. Right now its not a priority for me.

There are a lot of ways to do things. I like to try everything at least once but my methods are pretty set. When working on stuff that must succeed I do not experiment much. I save that for times when it doesn't matter if a project goes south.


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OfflineMachiavelliavore
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Re: So what happens if..... [Re: filthyknees]
    #22000319 - 07/26/15 03:01 PM (8 years, 6 months ago)

Quote:

filthyknees said:
I take a half quart of sterilized water, g2g in half a quart of spawn with blender/band assembly on top. Blend and expand. I tried this once and when I expanded it right away the results where 100%. I used WBS and blended about 10 seconds.




Desire to use acronyms... too strong.  Cannot.  Ahhhh. :oldman2::oldman:
g2w=g2win yo!

:blast:

Slurry seems like a pretty nice way to save some expansion steps in terms of noc'ing spawn jars.  Spawning with it directly seems uneeded since inoculation points aren't really a problem with most cube bulks.

I wonder if it would be super effective for oysters or woodlovers, something that uses a lot more substrate.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineGalba Cubensis
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Re: So what happens if..... [Re: Machiavelliavore]
    #22000640 - 07/26/15 04:30 PM (8 years, 6 months ago)

I know it depends on the ratio too ofc, but the recolonization time just seemed pretty sweet. How often do you get contams, considering only the "important projects" as you call it?


--------------------
RETARDS!  I'LL IGNORE ALL OF YOUR ADVICE!  BUT TELL ME WHAT TO DO STILL!


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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: Galba Cubensis]
    #22001127 - 07/26/15 06:13 PM (8 years, 6 months ago)

If I have all the vectors nailed down I usually only see contams from sloppy procedure maybe 5% of the time. If I need to change grain or switch rooms or try something new then I need to be extra careful how I do things.


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Invisiblemaddchef
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Re: So what happens if..... [Re: Pastywhyte]
    #22001265 - 07/26/15 06:39 PM (8 years, 6 months ago)

I still don't see why its a raised contam risk though. Some deal as LI essentially.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: maddchef]
    #22001321 - 07/26/15 06:54 PM (8 years, 6 months ago)

Because there is uncolonized starch being exposed. Its not a huge vector but it still exists.  If you recognize that it won't bite you as often.


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InvisibleMudaFuka
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Re: So what happens if..... [Re: Pastywhyte]
    #22001346 - 07/26/15 07:00 PM (8 years, 6 months ago)

If you are using it to inoculate more spawn or some bottle/bag subs in sterile conditions it's fairly safe. I was think more in terms of inoculating bulk subs.


--------------------
AMU
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Invisiblemaddchef
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Re: So what happens if..... [Re: Pastywhyte]
    #22001353 - 07/26/15 07:01 PM (8 years, 6 months ago)

I get it if you're pouring directly into substrate but if you're going into a jar or bag I don't see the added risk.

You either get contams in your receiving vessel while pouring LI or your slurry. Is there somehow a higher risk that I'm not understanding?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisiblemaddchef
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Re: So what happens if..... [Re: maddchef]
    #22001354 - 07/26/15 07:02 PM (8 years, 6 months ago)

Ahh fucker you read my mind. Thanks


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: maddchef]
    #22001365 - 07/26/15 07:04 PM (8 years, 6 months ago)

Sorry, of course there is no added vector if used as an inoculate of more grain and done properly. For some reason I though you guys were talking about spawning with the grain slurry.


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OfflineKizzle
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Re: So what happens if..... [Re: maddchef]
    #22001406 - 07/26/15 07:14 PM (8 years, 6 months ago)

What makes you think the center isn't colonized?


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Invisiblemaddchef
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Re: So what happens if..... [Re: Kizzle]
    #22001421 - 07/26/15 07:17 PM (8 years, 6 months ago)

Because for our purposes we tend to use grain as soon as the exterior is colonized so its likely for whole grains to not have been penetrated yet is my belief.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: Kizzle]
    #22001423 - 07/26/15 07:18 PM (8 years, 6 months ago)

What makes you think it is? Typically I don't leave my grain jars to consolidate much, if I did it might be a different story. The fact some people got blown away with contams when blending a lot then spawning in open air, while others just did a buzz or two with smaller grain to break off the bran, is an interesting observation that leads me to make the hypothesis that it takes longer for the center of the larger grains to colonize.


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Re: So what happens if..... [Re: Kizzle]
    #22001441 - 07/26/15 07:22 PM (8 years, 6 months ago)

There's no way that every single part of each grain will be colonised inside and out by the time a jar is ready to use. If it were there would be no nutrition left for fruiting.


--------------------
AMU
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Invisiblemaddchef
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Re: So what happens if..... [Re: MudaFuka]
    #22001478 - 07/26/15 07:31 PM (8 years, 6 months ago)

So blend the grain, let sit 2 days, blend again and go straight into bulk sub?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineKizzle
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Re: So what happens if..... [Re: Pastywhyte]
    #22001516 - 07/26/15 07:37 PM (8 years, 6 months ago)

For one you can often see mycelium growing out the center on split grains. Most the moisture and nutrients the mycelium is using to grow is in the center. The center of some grain changes color as it colonizes and you can see that happen almost as fast the mycelium covers the outside. If you look at starch taken from the center through a microscope you can mycelium. Also if you shake up spawn sometimes there's barely even any mycelium on the outside and yet look at how much mycelium grow back out of it after only a couple days. There's probably more in the center than on the outside by the time you spawn it.


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Invisiblemaddchef
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Re: So what happens if..... [Re: Kizzle]
    #22001544 - 07/26/15 07:42 PM (8 years, 6 months ago)

I would agree if it's from spores maybe but if it's inoculated with something such as lc there's very few days for it to reach into the center of non split grains.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMudaFuka
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Re: So what happens if..... [Re: Kizzle]
    #22001558 - 07/26/15 07:43 PM (8 years, 6 months ago)

Yes there is mycelium inside the grain but all the starches have not been bio converted into mycelium. So when the grains are blended those yet unused starches are released.


--------------------
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Re: So what happens if..... [Re: MudaFuka]
    #22001591 - 07/26/15 07:53 PM (8 years, 6 months ago)

I don't imagine it's any different than what happens when you crumble BRF cake. Not that I've actually tried grinding colonized grains before spawning but I haven't seen anyone explicitly try it and fail in any way either yet. And if the slurry thing works I can't imagine why grinding them without the liquid wouldn't work too.


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Edited by Kizzle (07/26/15 07:54 PM)


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Re: So what happens if..... [Re: Kizzle]
    #22001622 - 07/26/15 08:00 PM (8 years, 6 months ago)

Except people have tried. It was found that a higher rate of contamination was had with larger grains blended more. Millet based spawn blended minimally was found to be more successful.  Its all in eats thread if someone could link it, im on my phone.


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Re: So what happens if..... [Re: Pastywhyte]
    #22002102 - 07/26/15 09:40 PM (8 years, 6 months ago)

Well we can only guess as to what factors might influence it. Even if grinding grain has been tried there are a lot of different grains and they all have different compositions. Even water content can affect how the starch gelatinizes in the center during sterilization. There's a lot of potential experimentation left if someone's interested in trying it.


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Re: So what happens if..... [Re: Kizzle]
    #22002111 - 07/26/15 09:42 PM (8 years, 6 months ago)

Well I believe Eatyu is still working it out. He has had some good success so far so I am sure he will nail it down.


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Re: So what happens if..... [Re: Pastywhyte]
    #22002153 - 07/26/15 09:52 PM (8 years, 6 months ago)

Hell I got a bunch of spawn in my fridge. I could do some tests if my coffee grinder still works. I think I might have killed it when I was trying to grind up some rock hard guinea-pig turds :grin: It might just be clogged though.


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Re: So what happens if..... [Re: Kizzle]
    #22002454 - 07/27/15 12:00 AM (8 years, 6 months ago)

Need slury to noc cakes to make slury.  :suicide:  LI :smile:

I think the grass seed should do well.  It really seems to grow a shitton of mycellium between seeds, unlike millet.  All fuckin rubbery n shyte.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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