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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: Galba Cubensis]
    #22000112 - 07/26/15 02:03 PM (8 years, 6 months ago)

A comparison of what? Yields will be similar,  eat proved that. The difference is in speed of spawn run. You achieve full colonization in 2-3 days. Eat proved it. It carries a slightly higher risk of contam. Not sure what else is needed :shrug:


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OfflineGalba Cubensis
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Re: So what happens if..... [Re: Pastywhyte]
    #22000288 - 07/26/15 02:50 PM (8 years, 6 months ago)

Didnt know eat proved anything, there are some who say there are 10k pages, so there is still alot I havent "sorted through" and prob never will. Shouldnt you pros be doing this all the time then? Since, if I remember correctly, its how rarely you get contams that makes one pro?


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RETARDS!  I'LL IGNORE ALL OF YOUR ADVICE!  BUT TELL ME WHAT TO DO STILL!


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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: Galba Cubensis]
    #22000310 - 07/26/15 02:56 PM (8 years, 6 months ago)

I grow the way I grow. Eat grows his own way. Both are good. I change things up and try stuff but I have been busy doing my own thing. Maybe later I will try slurry more in depth. Right now its not a priority for me.

There are a lot of ways to do things. I like to try everything at least once but my methods are pretty set. When working on stuff that must succeed I do not experiment much. I save that for times when it doesn't matter if a project goes south.


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OfflineMachiavelliavore
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Re: So what happens if..... [Re: filthyknees]
    #22000319 - 07/26/15 03:01 PM (8 years, 6 months ago)

Quote:

filthyknees said:
I take a half quart of sterilized water, g2g in half a quart of spawn with blender/band assembly on top. Blend and expand. I tried this once and when I expanded it right away the results where 100%. I used WBS and blended about 10 seconds.




Desire to use acronyms... too strong.  Cannot.  Ahhhh. :oldman2::oldman:
g2w=g2win yo!

:blast:

Slurry seems like a pretty nice way to save some expansion steps in terms of noc'ing spawn jars.  Spawning with it directly seems uneeded since inoculation points aren't really a problem with most cube bulks.

I wonder if it would be super effective for oysters or woodlovers, something that uses a lot more substrate.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineGalba Cubensis
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Re: So what happens if..... [Re: Machiavelliavore]
    #22000640 - 07/26/15 04:30 PM (8 years, 6 months ago)

I know it depends on the ratio too ofc, but the recolonization time just seemed pretty sweet. How often do you get contams, considering only the "important projects" as you call it?


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RETARDS!  I'LL IGNORE ALL OF YOUR ADVICE!  BUT TELL ME WHAT TO DO STILL!


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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: Galba Cubensis]
    #22001127 - 07/26/15 06:13 PM (8 years, 6 months ago)

If I have all the vectors nailed down I usually only see contams from sloppy procedure maybe 5% of the time. If I need to change grain or switch rooms or try something new then I need to be extra careful how I do things.


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Invisiblemaddchef
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Re: So what happens if..... [Re: Pastywhyte]
    #22001265 - 07/26/15 06:39 PM (8 years, 6 months ago)

I still don't see why its a raised contam risk though. Some deal as LI essentially.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: maddchef]
    #22001321 - 07/26/15 06:54 PM (8 years, 6 months ago)

Because there is uncolonized starch being exposed. Its not a huge vector but it still exists.  If you recognize that it won't bite you as often.


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InvisibleMudaFuka
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Re: So what happens if..... [Re: Pastywhyte]
    #22001346 - 07/26/15 07:00 PM (8 years, 6 months ago)

If you are using it to inoculate more spawn or some bottle/bag subs in sterile conditions it's fairly safe. I was think more in terms of inoculating bulk subs.


--------------------
AMU
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Invisiblemaddchef
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Re: So what happens if..... [Re: Pastywhyte]
    #22001353 - 07/26/15 07:01 PM (8 years, 6 months ago)

I get it if you're pouring directly into substrate but if you're going into a jar or bag I don't see the added risk.

You either get contams in your receiving vessel while pouring LI or your slurry. Is there somehow a higher risk that I'm not understanding?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisiblemaddchef
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Re: So what happens if..... [Re: maddchef]
    #22001354 - 07/26/15 07:02 PM (8 years, 6 months ago)

Ahh fucker you read my mind. Thanks


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: maddchef]
    #22001365 - 07/26/15 07:04 PM (8 years, 6 months ago)

Sorry, of course there is no added vector if used as an inoculate of more grain and done properly. For some reason I though you guys were talking about spawning with the grain slurry.


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OfflineKizzle
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Re: So what happens if..... [Re: maddchef]
    #22001406 - 07/26/15 07:14 PM (8 years, 6 months ago)

What makes you think the center isn't colonized?


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Invisiblemaddchef
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Re: So what happens if..... [Re: Kizzle]
    #22001421 - 07/26/15 07:17 PM (8 years, 6 months ago)

Because for our purposes we tend to use grain as soon as the exterior is colonized so its likely for whole grains to not have been penetrated yet is my belief.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePastywhyteMDiscord
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Re: So what happens if..... [Re: Kizzle]
    #22001423 - 07/26/15 07:18 PM (8 years, 6 months ago)

What makes you think it is? Typically I don't leave my grain jars to consolidate much, if I did it might be a different story. The fact some people got blown away with contams when blending a lot then spawning in open air, while others just did a buzz or two with smaller grain to break off the bran, is an interesting observation that leads me to make the hypothesis that it takes longer for the center of the larger grains to colonize.


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InvisibleMudaFuka
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Re: So what happens if..... [Re: Kizzle]
    #22001441 - 07/26/15 07:22 PM (8 years, 6 months ago)

There's no way that every single part of each grain will be colonised inside and out by the time a jar is ready to use. If it were there would be no nutrition left for fruiting.


--------------------
AMU
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Invisiblemaddchef
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Re: So what happens if..... [Re: MudaFuka]
    #22001478 - 07/26/15 07:31 PM (8 years, 6 months ago)

So blend the grain, let sit 2 days, blend again and go straight into bulk sub?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineKizzle
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Re: So what happens if..... [Re: Pastywhyte]
    #22001516 - 07/26/15 07:37 PM (8 years, 6 months ago)

For one you can often see mycelium growing out the center on split grains. Most the moisture and nutrients the mycelium is using to grow is in the center. The center of some grain changes color as it colonizes and you can see that happen almost as fast the mycelium covers the outside. If you look at starch taken from the center through a microscope you can mycelium. Also if you shake up spawn sometimes there's barely even any mycelium on the outside and yet look at how much mycelium grow back out of it after only a couple days. There's probably more in the center than on the outside by the time you spawn it.


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Invisiblemaddchef
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Re: So what happens if..... [Re: Kizzle]
    #22001544 - 07/26/15 07:42 PM (8 years, 6 months ago)

I would agree if it's from spores maybe but if it's inoculated with something such as lc there's very few days for it to reach into the center of non split grains.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMudaFuka
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Re: So what happens if..... [Re: Kizzle]
    #22001558 - 07/26/15 07:43 PM (8 years, 6 months ago)

Yes there is mycelium inside the grain but all the starches have not been bio converted into mycelium. So when the grains are blended those yet unused starches are released.


--------------------
AMU
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