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Offlineonehunglow
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Panellus Stipticus Project
    #18714043 - 08/16/13 10:50 AM (10 years, 5 months ago)

Alright, so this is my first mushroom grow project and also my first post on this forum.

In summary, i want to grow Panellus Stipticus because of its bio-luminescence. I've seen all the forum posts on this site about the species and I've been super impressed.
I recently purchased a bag of 100 Panellus Stipticus plug spawn from
glowmushroom.com. The owner was very nice and super helpful. What i am shooting for is to grow these mushrooms in a large globe vase i recently purchased. I thought this vase would be perfect for displaying the beautiful glow the mycellium and mushrooms produce. I also purchased a glass lid that can be placed on top of this vase in case i need to seal it off.

.

The plug spawn should arrive tomorrow and I'm just wondering where i should take it from there. My goal would be to have this entire vase glowing. I also purchased 3lbs of hardwood oak pellets from
The spore depot

I also obtained a large rotting hardwood stump that was sitting in the mud under a workbench in a nursery. I washed it off, broke the whole stump into really small bits and wood chips, and baked in in the oven for 1.5hrs to dry it off and sterilize it (may have been too long, i dont know).

So i was thinking about filling this vase with the oak pellets, hardwood log bits, and the plug spawn i purchased, in the hopes that the mycellium would eventually spread to the pellets and woodchips.

Any ideas? any critiques? Im totally open for anything, but would prefer not to spend much more money on anything. Also, i read that lowing the pH to 3.6 with citric acid or lemon juice makes them glow much brighter, however i have no idea how i would go about doing this. I have no experience in that area, however i can easily learn.

In summary, i want this vase to be filled with growing pannellus stipticus and glow at night. I live in san diego, and they will be growing in a fairly warm room year round.


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OfflineTmethylM
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Re: Panellus Stipticus Project [Re: onehunglow]
    #18714096 - 08/16/13 11:10 AM (10 years, 5 months ago)

You've made a few minor mistakes already, but you have what you need and we can make it work.
1) plug spawn is not a good idea for this project, what you needed was grain spawn.
2) you should've soaked the wood for a few days and sterilized it in a sealed oven bag or something to prevent moisture loss.

Not a big deal though, we can make this work.


First thing is get those wood chips soaking asap.

I can help more but I'm on an extremely tight schedule right now.


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Offlineonehunglow
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Re: Panellus Stipticus Project [Re: Tmethyl]
    #18714180 - 08/16/13 11:35 AM (10 years, 5 months ago)

Thanks for the fast response, looking forward to any help with this project you can give me.

It may be possible for me to return the dowels i ordered but it would be nice if i didnt have to.
Being a first timer, i am unaware of the advantages of grain spawn for this project vs the dowels.
If so what would be my next course of action?

I just placed the wood chips in a large bowl filled with room temp water.
Ill let them soak for two days or so.


Edited by onehunglow (08/16/13 11:42 AM)


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OfflineTmethylM
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Re: Panellus Stipticus Project [Re: onehunglow]
    #18714238 - 08/16/13 11:51 AM (10 years, 5 months ago)

The problem with dowels is they are meant for outdoor stump inoculation and you typically won't be harvesting until the next season.
They have little surface area, in other words you cant exactly evenly spread a dowel through a substrate, you can with grains.
If you decide to use the dowels that is fine, they will take slightly longer to colonize your vase though.


Now that you have the wood soaking,  you can get started on sterilizing your jar/lid, then leave it sealed until you add the wood and inoculant.
Heat sterilization isn't quite necessary though, a wipe down with 70% I do alcohol will be sufficient.

I'll be back :lol:

Also welcome to the shroomery my friend.


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Offlineonehunglow
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Re: Panellus Stipticus Project [Re: Tmethyl]
    #18714270 - 08/16/13 11:58 AM (10 years, 5 months ago)

Thanks, that makes perfect sense.
Well, if it means i can harvest faster, i can certainly return these dowels and use a different method for this project. However, i spent $30 on the bag of dowels, and would like to keep my budget at $30.

Will do on the sterilization, thanks again.


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Offlineforrest
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Re: Panellus Stipticus Project [Re: onehunglow]
    #18714699 - 08/16/13 01:23 PM (10 years, 5 months ago)

grain spawn is also a better innoculant than dowels because it has way more nutrition (nitrogen especially), with makes the mycelium take over the wood/sawdust much faster, minimizing the chances of unwanted contaminants.

but good luck!


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Offlineonehunglow
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Re: Panellus Stipticus Project [Re: forrest]
    #18714734 - 08/16/13 01:32 PM (10 years, 5 months ago)

Well,

I just received the dowels in the mail as promised.
If i were to return the dowels, and purchase a spore syringe (which would be my first time doing so), any estimate of how much the total of this project, for the size of my globe vase, would run up to?
Would it be possible for me to buy the supplies i need already prepared online?

I would love to spend no more than $30 on this project if i worked with grain. Any suggestions forrest?


Edited by onehunglow (08/16/13 01:35 PM)


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InvisibleRas Rising
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Re: Panellus Stipticus Project [Re: onehunglow]
    #18714776 - 08/16/13 01:42 PM (10 years, 5 months ago)

Quote:

onehunglow said:
Well,

I just received the dowels in the mail as promised.
If i were to return the dowels, and purchase a spore syringe (which would be my first time doing so), any estimate of how much the total of this project, for the size of my globe vase, would run up to?
Would it be possible for me to buy the supplies i need already prepared online?

I would love to spend no more than $30 on this project if i worked with grain. Any suggestions forrest?




i would say $30 is defiantly doable or close to it. http://www.shroomery.org/sponsors.php i suggest getting your spores from one of these guys ^^^^ a shroomery sponsor. I personally like the spore depot. Syringe: $12. jar and spawn like 10 or so if that.


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Offlineonehunglow
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Re: Panellus Stipticus Project [Re: Ras Rising]
    #18714792 - 08/16/13 01:47 PM (10 years, 5 months ago)

Hmm i see no Panellus stipticus spores on Thesporedepot.
Can you direct me to a $12 site? also, how much grain would i need to buy to fill up this globe vase eventually i think i can just pick up jars at a store nearby.


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Offlineforrest
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Re: Panellus Stipticus Project [Re: onehunglow]
    #18714863 - 08/16/13 02:05 PM (10 years, 5 months ago)

i don't know about a 12 dollar site, but sporeworks has panellus stipticus liquid culture syringes.
but then you would have to make your own grain spawn (including have a pressure cooker), or maybe make pf-tek jars and use those as spawn.
considering you mentioned that this is your first mushroom grow project, there is a chance of failure when doing your first grain or pf prep. wich would eventually lead to more money spend/wasted.
so then you would be better off ordering colonised grain.
but if thimothy says it's doable with plugspawn, it probably isn't that bad of an option.


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Offlineonehunglow
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Re: Panellus Stipticus Project [Re: forrest]
    #18715063 - 08/16/13 03:05 PM (10 years, 5 months ago)

Thanks,

I hear what you're saying, and i definitely don't want anything to get contaminated.

I've seen lots of sites with Sterilized grain spawn bags online.
If i were to completely scrap my dowel idea, and start fresh with sterilized grain spawn and a spore syringe, how would i go about eventually transfering the grain with mycellium growing/ready to fruit to my globe vase? and what else should be in the globe vase...

As i said, i want this vase to be glowing as much as i can get it, even if its packed.


Edited by onehunglow (08/16/13 03:34 PM)


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Offlineforrest
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Re: Panellus Stipticus Project [Re: onehunglow]
    #18715228 - 08/16/13 03:49 PM (10 years, 5 months ago)

i'm haven't grown panellus stipticus and am not so experienced with sawdust/wood, so probably others as timothy can awnser those questions better. good luck mate!


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OfflineTmethylM
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Re: Panellus Stipticus Project [Re: onehunglow]
    #18715519 - 08/16/13 04:58 PM (10 years, 5 months ago)

Quote:

onehunglow said:
Thanks,

I hear what you're saying, and i definitely don't want anything to get contaminated.

I've seen lots of sites with Sterilized grain spawn bags online.
If i were to completely scrap my dowel idea, and start fresh with sterilized grain spawn and a spore syringe, how would i go about eventually transfering the grain with mycellium growing/ready to fruit to my globe vase? and what else should be in the globe vase...

As i said, i want this vase to be glowing as much as i can get it, even if its packed.




Get sterilized grain spawn, inoculate with Pan stip syringe (few CC's will do)
Allow to colonize, once the bag is 20-35% colonized, you mix the bag very well. You use your hands to squish the grains around and knead them.
Once the bag is 100% colonized (solid white) you will then prepare your substrate, which in your case is wood.

Now, into your vase you poor a handful of sterilized wood, then a handfull of colonized grains, and repeat, until the vase is completely full, you can pack it lightly to fit more, but don't pack it super hard, this will trap gas exchange and your culture may stall out. If you have some left over, just make another one :lol:


Seem simple enough?


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Offlineonehunglow
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Re: Panellus Stipticus Project [Re: Tmethyl]
    #18715703 - 08/16/13 05:44 PM (10 years, 5 months ago)

I will definitely do this instead, ill purchase what i need in a minute.

After my wood is done soaking, would i still need to bake it in the oven again? and this time with a oven bag?

Also, how would i sterilize the oak pellets i purchased?

Lastly, how large of a order of sterilized grain would i need? and any recommendations seller wise? 1lb? 2lb's? more?

Also, about citric acid/ lemon juice to make them glow brighter, would i do this now? or later?


Edited by onehunglow (08/16/13 05:45 PM)


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OfflineTmethylM
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Re: Panellus Stipticus Project [Re: onehunglow]
    #18715735 - 08/16/13 05:51 PM (10 years, 5 months ago)

Yes, in a sealed oven bag, just use a clip to keep it closed during baking.
Treat wood pellets the same as any other wood, soak then sterilize in oven bag or pressure cooker.

1 lb of spawn will be more than enough for a few vases.
Get more if you want to do more.

I would just add 1tblspn of citric acid to your wood/pellets soaking water.
The wood will absorb it, but you don't want to use too much, 1tblspn is plenty in my opinion.


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Offlineonehunglow
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Re: Panellus Stipticus Project [Re: Tmethyl]
    #18715749 - 08/16/13 05:54 PM (10 years, 5 months ago)

You've been amazingly helpful.

Ill go ahead and order a few things and post pictures as i go along.

Thanks!


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Offlineonehunglow
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Re: Panellus Stipticus Project [Re: onehunglow]
    #18722516 - 08/18/13 11:39 AM (10 years, 5 months ago)

I have two questions, how long should i bake both my woodchips and oak pellets in the oven after soaking?

Also, when they are done, shall i store them in my sanitized vase? if so with lid on? or off?


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OfflineTmethylM
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Re: Panellus Stipticus Project [Re: onehunglow]
    #18724352 - 08/18/13 07:22 PM (10 years, 5 months ago)

I'd bake them at 300F for 1.5hours to sterilize thoroughly.
Leave them inside the oven bags, sealed, until it's time to mix them with the colonized spawn.


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InvisibleFrankHorrigan
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Re: Panellus Stipticus Project [Re: Tmethyl]
    #18724921 - 08/18/13 09:35 PM (10 years, 5 months ago)

Why not just grow them on BRF cakes?  They grow (and glow) quite well on them.

Otherwise I would just use sawdust jars/bags...seems a bit easier :shrug:


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Offlineonehunglow
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Re: Panellus Stipticus Project [Re: FrankHorrigan]
    #18726469 - 08/19/13 08:27 AM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
Why not just grow them on BRF cakes?  They grow (and glow) quite well on them.

Otherwise I would just use sawdust jars/bags...seems a bit easier :shrug:





Thanks, i could but this grain project is happening.


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Offlineonehunglow
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Re: Panellus Stipticus Project [Re: Tmethyl]
    #18871502 - 09/21/13 10:37 AM (10 years, 4 months ago)

Quote:

Tmethyl said:
I'd bake them at 300F for 1.5hours to sterilize thoroughly.
Leave them inside the oven bags, sealed, until it's time to mix them with the colonized spawn.




Well, both grain bags are just about %100 percent colonized. When i mix them with my bulk substrate, do i leave my large globe vase unsealed atop? or seal it with foil and put glass plate ontop?


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Offlineonehunglow
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Re: Panellus Stipticus Project [Re: onehunglow]
    #18875119 - 09/22/13 09:07 AM (10 years, 4 months ago)

bump


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