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Offlinepkilly126
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Grow Log #2: Ecuadorian/Malabar Monotubs
    #18710217 - 08/15/13 05:06 PM (10 years, 6 months ago)

PK's Grow Log #2 -- Grain (WBS) Prep

My First Grow Log - Just Finished!

    I started at 9:30am & was done before 10:00am using TL's WBS Grain Prep Tek

For those like me who've used Doc34's WBS Tek in the past the steps are different with the end-goal being the same. I like to experiment. So, the Steps I took..
  • Measured Out 1.25 Cups Dry Wbs/ Quart Jar (6 Jars' worth or 7.5 Cups per pot)
  • Rinsed off starches,dust etc. using a colander as per TL's pics, I really like to get my hands in there and make sure everything feels right
  • After the h2o draining is clear & you're satisfied use your colander & dump the seeds back into your clean pot
  • While I didn't add any Gypsum, I did add 1 pint liquid coffee (drinking strength) to the first 2/3 pots' water (12 Jars worth)
  • Cover seeds with hot water & mix coffee/gypsum as per preference TL suggests 1 pint coffee/4 quarts WBS
  • Cover all pots and let soak 14-20 hours to get those endospores germinated & vulnerable
  • Smoke somethin & get excited about cookin up your grains tonight! :prettyflyforawhiteguy: 







11:35am EDIT: 1 pint coffee added to pot #3, all grains now have a pint of added liquid coffee to their soak water, quickly added & stirred


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: pkilly126]
    #18710357 - 08/15/13 05:34 PM (10 years, 6 months ago)

I love these grow logs! I'll definitely be keeping an eye on this thread :smile:


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: d9c6]
    #18714201 - 08/16/13 11:41 AM (10 years, 6 months ago)

:popcorn:


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Offlinepkilly126
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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: TranscendingLife]
    #18717568 - 08/17/13 03:00 AM (10 years, 6 months ago)

PK's Grow Log #2 - Inoculation

    So after allowing all grains to soak for 14 hours..
  • While waiting all day I made up my jars with a tyvek (Post Office Tyvek) filter, a 1/4" hole & Micropore tape. I kept them in my closet In their cardboard box they came in, bad mistake WARNING If you do this be really careful because there were some wood-eating mites(?) all throughout my jars! Not too fun a surprise when you're about to start PCing.
  • This is how I like my sink setup: Tongs for taking out sterile jars, Metal Spoon for scooping grains in the jar (keeps em off the brim/lid), Foil for.. well foil! And of course my trusty Colander. Dry, Scoop, Foil, Load; all right there:cheers:
  • As Per TL's Tek threw on the burner in Pot 1.
  • Immediately upon boiling (after stirring 1x/5min as suggested) tossed into the colander to steam dry, tossing up, down & side-to-side frequently. Grains felt real starchy (damn corn!) but at this point I had to let em steam dry. Should've rinsed more before the soak too probably but it shouldn't be a problem.
  • After about 45min my grains were dry to the touch so I proceeded to load them up into their respective jars & wait for the magic 15 PSI at which time count down from 90 min. begins.
  • After 3 cycles of this (leave the last batch in the PC overnight) and 5 hours of sleep later I got up, took a shower & bombed my room w/ Lysol, bombed the workstation with my Iso spray bottle & wiped everything down even the lighter I'd be using to flame and the Micropore tape.
  • after shaking the shit out of my remaining Ecuador & new Malabar MS Syringe I had a little help from a friend who (w/ sterile hands) cut me Micropore tape squares (hard w/ gloves) and flamed the lighter. In this fashion 18 jars, 1 at a time, 6 quart jars per planned tub were carefully inoculated and now sit on a shelf.


Note: What the hell were those mites? I was OCD thorough after I discovered them & they seemed to source from the cardboard (& I hope not the freakin' Verm/Grains/Coir Bricks I have in there too!) that was shipped here.. idk.
      Also I didn't inoculate 5 hours after PCing as it may seem to read.. I went to bed right after round 3 hit 0 PSI & 5 hours later I awoke but did not knock the jars up right away.


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: pkilly126]
    #18720157 - 08/17/13 07:16 PM (10 years, 6 months ago)

No idea about the mites.
As long as they're in the jars before loading them and you rinsed the jars out, you'll be fine.
If they weren't in the jars, then no need to worry at all.

Did you really PC your grains three times for 90 minutes @ 15 PSI?
That's way overkill. Just do one run for 120 minutes @ 15 PSI.


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Offlinepkilly126
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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: TranscendingLife]
    #18728158 - 08/19/13 03:19 PM (10 years, 6 months ago)

PK's Grow Log #2: Update


  Haha no TL I cooked 3 batches of grains. I have the Presto 23Qt. so I cook 3 batches of 6 quart jars for a total of 18, I just cycle them one after the other I have 3 separate pots soakin grains, y'know? Sorry if I made it sound that way:smirk:
  Holy shit could you even use a 3x cooked grain jar? It's a long ass process too because you have to wait for it to get up to 15psi while other grains are soakin & drying & it's damn hard to time that right!
  In the very near future I'll be remedying this by buying another PC.. at the very least the same one I currently have but I'd really like to get an AA-41(2?) Quart PC, everytime I see one on here I feel like Homer Simpson lookin at donuts :haha:

Progress:
  • Keeping the area clean with Lysol bombs & My lovely lil Iso bottle
  • Batches #1 & #2 are showing nice white mycelium poofs already in every jar however, Batch #3 has no visible growth as of yet and somehow the grains appear different although this could've simply been a result of lighting.. more on this later
  • Haven't seen any of those little bastards I found in my jar cases, thank God!:discorex:
  • I'll be checking in every day or two (not necessary per se but I personally like to know what's going on every day to maintain control besides, I have to spray it down so why not?
  • Mold keeps re-appearing in my bathroom on my shower curtains inside.. I'd like to eradicate it, for good. Every time I shower I don't feel 100% comfortable "working" until I dry, dress & sanitize. 


I'll try to get some pics up as the mycelium spreads, I'd like as much as possible to make this a pictorial journal since it's way easier & entertaining for you guys. In that spirit, hopefully I'll be getting a smartphone very soon. Thanks!:dancingbacon:


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Edited by pkilly126 (08/19/13 03:29 PM)


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: pkilly126]
    #18732490 - 08/20/13 02:04 PM (10 years, 6 months ago)

AA941 are 41 quart PCs.
I run an AA930, which fits 14 quart jars.


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: pkilly126]
    #18733257 - 08/20/13 05:11 PM (10 years, 6 months ago)

Quote:

pkilly126 said:
I have the Presto 23Qt. so I cook 3 batches of 6 quart jars for a total of 18



You should be able to fit 10 qts in the 23qt presto. 7 standing, 3 laying across the top of the 7. You could get those 18 jars PCd in only 2 runs instead of 3.


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Offlinepkilly126
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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: Juiceh]
    #18738436 - 08/21/13 04:23 PM (10 years, 6 months ago)

Quote:

TranscendingLife said:
AA941 are 41 quart PCs.
I run an AA930, which fits 14 quart jars.




That's awesome.. not bad at all & surely the 930 would be a bit less than the 941. I really like AA's stuff it just screams "quality" at you Presto's not bad though

now..

Quote:

Juiceh said:
Quote:

pkilly126 said:
I have the Presto 23Qt. so I cook 3 batches of 6 quart jars for a total of 18



You should be able to fit 10 qts in the 23qt presto. 7 standing, 3 laying across the top of the 7. You could get those 18 jars PCd in only 2 runs instead of 3.




that's incredible! I could fit that 7th one in the middle for sure but since i do 6 quarts/tub it's easier for inoculation purposes to be able to PC only a whole # of tubs' spawn like say 1 or 2 tubs worth of spawn. I never thought of that honestly and I'd be worried about the glass cracking more than anything doing that, does it? At any rate thanks for the suggestions.


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: pkilly126]
    #18738486 - 08/21/13 04:34 PM (10 years, 6 months ago)

Quote:

pkilly126 said:
that's incredible! I could fit that 7th one in the middle for sure but since i do 6 quarts/tub it's easier for inoculation purposes to be able to PC only a whole # of tubs' spawn like say 1 or 2 tubs worth of spawn. I never thought of that honestly and I'd be worried about the glass cracking more than anything doing that, does it? At any rate thanks for the suggestions.



Why would you only make just enough for a tub or 2? What if you have some jars stall? Why not have extras as insurance or to continue expansion via G2G? What would make the glasses any more likely to break? I haven't had any issues.


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Offlinepkilly126
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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: Juiceh]
    #18741192 - 08/22/13 08:19 AM (10 years, 6 months ago)

:amazedohmygod: You're absolutely right!.. In fact I'm going to be trying my hand at GLC once I get the requisite equipment. Stoner moment if I've ever seen one but thanks. I know firsthand too I lost 3 jars last round & one tub had the amount of spawn cut in half as a result so extra is if nothing else, insurance of sorts haha


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: pkilly126]
    #18772874 - 08/29/13 12:21 PM (10 years, 5 months ago)

PK's Grow Log #2 - Update


  • I realized I didn't inoculate a jar! (it was hidden in the back w/ foil on it still from PCing) so after debating whether or not to throw it out I decided to knock it up and I'm glad I did; A nice little puff of mycelium is developing visibly w/o anything else.
  • For the last 6 jars I PCed I added 1.5 liters (1cup/4quarts wbs) of liquid coffee to their soak water prior whereas the others only received one liter. These 6 are the slowest colonizing and the difference is staggering. I have jars (Batches #1 & 2) that're 50% or more that I could definitely shake, whereas Batch #3's maybe 15/20% along in the best jar? Could the coffee have been too strong I thought the grains appeared somewhat darker than the others right out of the PC
  • I've got all my LC/GLC & some agar equipment finally! Scalpel, Syringes (60cc & 10cc + 16 & 14 gauge 1.5" needles & one 3.5" LC needle since idk what's best..) RTV, Alcohol lamp, SFDs, Gypsum & Hydrated Lime, LME, Parafilm, Pyrex Culture tubes & petris and ready-made polypropylene LC & Spawn jar Lids.:crazy2:
  • I'm desperately trying to get pictures and I will possibly today with a friend's help
  • I may up this to 6 tubs or try to get a continual harvest type setup going once I inoculate my LC (just LME) & make a GLC


Questions

  • How long must a LC colonize before it's good to inject into grain jars?
  • Assuming consistent dispersal of mycelium (magnetic stirrer) how does one gauge the amount to use for inoculation vs say a MS syringe where I know 1cc is ok 2cc is what I want, etc..
  • GLC is suspended mycelium in sterile water, for how long & how should it be stored before LC nutrients must be added by aspirating one into the other?
  • Why do many experienced people seem to prefer Agar or G2G over GLC/LC? It seems more prone to contamination for purposes of endless inoculant for sure but, if it's all about isolation & genetic libraries I totally get it.

As Always Thank You & More to Come...:mushroom2:


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: pkilly126]
    #18773173 - 08/29/13 01:43 PM (10 years, 5 months ago)

I let my LCs colonize, without a stir bar, for 7-14 days before use.

GLCs are ready to use in a 1-3 days, or immediately, but I like allowing for the mycelium to "heal" after being extracted.

Agar is a great way to see/notice contams in your cultures & isolate away from them, thus ensuring a clean culture.
G2Gs are way quicker than inoculating with needles.
I can G2G 1 jar to 12 in less time than I use a needle to inoculate 12 jars.
Your thinking of "adding more times for contams to enter when using G2G or agar" is false.
There's actually less chance of failure/contams during agar work &/or G2Gs than (G)LC uses.

Think about it this way:
Print to agar-->agar-->master grain-->G2G-->G2G
Print to syringe-->(G)LC-->grain jars-->bulk

In the second manner, you have a lot more transfers & movements that require sterilization.
There's a lot more movement of tools in & out of jars and in your work area.

I can't remember the last time I touched, let alone, used a syringe.


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Offlinepkilly126
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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: TranscendingLife]
    #18774691 - 08/29/13 07:22 PM (10 years, 5 months ago)

Quote:

TranscendingLife said:
I let my LCs colonize, without a stir bar, for 7-14 days before use.

GLCs are ready to use in a 1-3 days, or immediately, but I like allowing for the mycelium to "heal" after being extracted.

Agar is a great way to see/notice contams in your cultures & isolate away from them, thus ensuring a clean culture.
G2Gs are way quicker than inoculating with needles.
I can G2G 1 jar to 12 in less time than I use a needle to inoculate 12 jars.
Your thinking of "adding more times for contams to enter when using G2G or agar" is false.
There's actually less chance of failure/contams during agar work &/or G2Gs than (G)LC uses.

Think about it this way:
Print to agar-->agar-->master grain-->G2G-->G2G
Print to syringe-->(G)LC-->grain jars-->bulk

In the second manner, you have a lot more transfers & movements that require sterilization.
There's a lot more movement of tools in & out of jars and in your work area.

I can't remember the last time I touched, let alone, used a syringe.



That's awesome man thanks you broke it down good & simple.. makes sense if you put it that way, I haven't yet learned how to take/work with prints yet though.
  Your GLCs (just mycelium & water?) will keep for a few months in the fridge?! (read this in a few threads)
  I'm going to do a master grain jar for GLC & LC just to familiarize and experiment. Until I get a Laminar Flow Hood I don't think I'll be doing agar/g2g. Granted I clean regularly & take every precaution possible when "working" but idk if that's good enough, do you use a LFH? A lot of people make them sound necessary for good success rates with that..


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: pkilly126]
    #18776848 - 08/30/13 10:54 AM (10 years, 5 months ago)

A GLC should be good for a couple of months in the fridge.
You extract a bit of nutrients from the grains during extraction of the mycelium.

I use an SAB for all my work.
https://www.shroomery.org/forums/showflat.php/Number/13552603#13552603
https://www.shroomery.org/forums/showflat.php/Number/17218726


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs [Re: TranscendingLife]
    #18789014 - 09/02/13 10:18 AM (10 years, 5 months ago)

PK's Grow Log #2: Pictorial Update


Here are some pictures taken yesterday, The ones w/ the jars in hand look at the bacteria (?) growing.. I have 100% colonized jars from that same night & I have some that have no mycelium on em.. @ what point do I get rid of em?








LC/Agar Supplies






Sorry about including the Vendor's name, I should've taped it out.. I don't only support them though so for future reference I apologize.


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Edited by pkilly126 (09/02/13 10:31 AM)


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs - Spawn-To-Bulk [Re: pkilly126]
    #18861854 - 09/19/13 07:43 AM (10 years, 5 months ago)

PK's Grow Log 2: Spawning to Bulk



  • So today & tonight I'll be pasteurizing 1 Brick of Coco Coir & 2.5qts Verm I also have gypsum which I'd like to add, I'll be searching like a madman today to brush up, the last time I did this I messed the h2O content up rather badly which caused delayed colonization (no contams thank god) so I'm going to do this right this time.

  • Unlike I thought I'll be doing 2 tubs not 3 as originally intended (NOTE: Now I get why you cook as many grain jars as possible) i lost 4 jars from 1 tub so I'm using the remaining 2 jars to make the other 2 tubs have 7qt spawn instead of having a weak ass third tub...

  • As always I'll TRY to get some damn decent pictures in... don't hold your breath though.. this log didn't look like i wanted it too.. i will edit this to be picture heavy or just give it another go. off to see if i can get an iphone & prepping for this. :prettyflyforawhiteguy:

Wish me luck fellow Shroomerites, although there's not much luck in this hobby there's plenty of carefully calculated risks that yield rewards to the diligent


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Re: Grow Log #2: Ecuadorian/Malabar Monotubs - Spawn-To-Bulk [Re: pkilly126]
    #18862803 - 09/19/13 12:15 PM (10 years, 5 months ago)

Quote:

pkilly126 said:
  • Unlike I thought I'll be doing 2 tubs not 3 as originally intended (NOTE: Now I get why you cook as many grain jars as possible) i lost 4 jars from 1 tub so I'm using the remaining 2 jars to make the other 2 tubs have 7qt spawn instead of having a weak ass third tub...




  • You could take the 2 jars that colonized first(or any 2 fully colonized jars) and G2G them into your next batches of PCd grains. One qt jar can easily inoculate a dozen more via G2G. Or you can G2G them into fewer qts and have faster colonization. I've had qts colonize in under a week after g2g. You could be ready to spawn another tub in a week. :wink:


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    Re: Grow Log #2: Ecuadorian/Malabar Monotubs - Spawn-To-Bulk [Re: Juiceh]
        #18862986 - 09/19/13 01:03 PM (10 years, 5 months ago)

    Ive had jars that didnt completely colonize that i spawned with and never had any problems. It will eventually colonize unless it looks like theres bacteria in it.


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    Re: Grow Log #2: Ecuadorian/Malabar Monotubs - Update S->B [Re: tonpole]
        #18868299 - 09/20/13 03:45 PM (10 years, 5 months ago)

    Tonpole - I went ahead and made the 3 tubs anyway, I used 6qts spawn in the first 2 and 3 qts (2 not being fully colonized and experimentally open air g2g). That isn't at all sterile on the last tub, that being said I didn't detect any funky smells it smelled like good ole mycelium and what I thought was cobweb I now believe to be new/weak growth.. as I've said a million times I'd have an easier time once I got a camera so you folks can actually give me feedback but we'll see..

    PK's Grow Log 2: Update


    • Tyvek suits are great but when your rooms already hot man do they make you sweat buckets in there which is not good if your sleeves expose your arm at any point but anyway I used the suit for dumping the gypsum infused coir/verm mix and of course for spawning the jars.. I'd unscrew them as i needed get everything i could with my gloved hand & put the lid back on immediately (PS-A little whiff of the jar seems to go a long way)
    • Used Damion5050's Tek since it worked before but I (again) used 2.5qts verm instead of 2 and as TL suggests I put a 1/4 cup gypsum into my boiling water before adding it to the bucket
    • I've been taking precautions in my room prior to arranging this, I have a HEPA filter (TrueHEPA) running on high most of the time but i cut it off for still air and i bombed the room with lysol prior to starting and secluded the tubs & buckets after I'd cleaned the tubs with water, soap & bleach in my room and sprayed with iso after inside & out, where no one was for hours. I showered before beginning work and wore fresh clothes under a freshly washed-once-used-before tyvek suit & face sock, nitrile gloves washed in alcohol & changed every "tub" I did.
    • In that manner I simply waited for the coir/verm to cool 6/7 hours in the buckets, trying to keep opening the buckets to test temp to a minimum (2x) and once the time came I quickly undid the lid for #1, let the water to drip back into the mix then with my alcohol gloved handget all the water/coir mix from the sides to the main heap & dump it in, mix it up well, even it out, make sure it's fluffy & re-lid my  bucket and immediately one by one add the spawn jars as described above.. basically an "open-air" situation, I wasn't going to keep putting the lids on/off the tubs that seems like it would've been counterproductive and made me sweat even more (if such a thing were possible)
    • Well I gotta get pics of my LC's & GLC's (they appear to me to be ok but that's not saying too much as of now) and use the last of my WBS to keep the cycle going.. more on that in another thread.


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