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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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OfflineLoveLightPeace
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Registered: 08/26/11
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Slant tubes question
    #18707858 - 08/15/13 05:45 AM (10 years, 5 months ago)

What size slant tubes are best for keeping cultures in?

Also are centrifuges and slant tubes two different things, or two names for the same thing?


--------------------
I am so small I can barely be seen.
How can this great love be inside me?

Look at your eyes. They are small,
but they see enormous things.

(Rumi)


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OfflineStromriderM
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Re: Slant tubes question [Re: LoveLightPeace]
    #18707889 - 08/15/13 06:06 AM (10 years, 5 months ago)

As for your first question it has been asked many times on here and the consensus seems to be the bigger the better. Just utsf for more in depth info. As for your second ques well I think so but I am not sure. I am still learning all this stuff too


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OfflineRogerRabbitM
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Re: Slant tubes question [Re: LoveLightPeace]
    #18707897 - 08/15/13 06:09 AM (10 years, 5 months ago)

A centrifuge is a tool which spins at high speed to separate materials with different weights.

Use test tubes with at least a 25mm opening or it's too hard to get the cultures out.  I use 25mm X 125mm for short term use and 60mm X 250mm for long term storage.
RR


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OfflineStromriderM
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Re: Slant tubes question [Re: RogerRabbit]
    #18707911 - 08/15/13 06:15 AM (10 years, 5 months ago)

I assumed they were referring to centerfuge tubes which I think are just regular test tubes :shrug:


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OfflineLoveLightPeace
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Re: Slant tubes question [Re: Stromrider]
    #18713216 - 08/16/13 05:41 AM (10 years, 5 months ago)

Thnx Guys! I think knowledge must be light because i feel illuminated!:smile:

I guess my last question would be, are glass tubes better than plastic ones for keeping cultures in longer?


--------------------
I am so small I can barely be seen.
How can this great love be inside me?

Look at your eyes. They are small,
but they see enormous things.

(Rumi)


Edited by LoveLightPeace (08/16/13 05:42 AM)


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InvisibleFrankHorrigan
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Re: Slant tubes question [Re: LoveLightPeace]
    #18713382 - 08/16/13 07:13 AM (10 years, 5 months ago)

Quote:

LoveLightPeace said:
I guess my last question would be, are glass tubes better than plastic ones for keeping cultures in longer?




They are not better or worse.

I prefer PP5 (plastic) tubes because I do not like the rubber vacutainer stoppers. I like the snap-down cap.

If you get plastic just make sure they are autoclave-able.


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OfflineLoveLightPeace
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Re: Slant tubes question [Re: FrankHorrigan]
    #18713436 - 08/16/13 07:36 AM (10 years, 5 months ago)

thnx but dsnt plastic respirate as opposed to glass which dsnt? so wudnt this factor make glass a better choice for keeping culture longer?


--------------------
I am so small I can barely be seen.
How can this great love be inside me?

Look at your eyes. They are small,
but they see enormous things.

(Rumi)


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InvisibleFrankHorrigan
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Re: Slant tubes question [Re: LoveLightPeace]
    #18713471 - 08/16/13 07:51 AM (10 years, 5 months ago)

I've never had nor heard of such an issue. I keep hundreds of PP5 tubes in cold storage. They work great.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


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OfflineLoveLightPeace
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Re: Slant tubes question [Re: FrankHorrigan]
    #18715255 - 08/16/13 03:55 PM (10 years, 5 months ago)

Thnx, and also are u supposed to keep them in the refrigerator or freezer?


--------------------
I am so small I can barely be seen.
How can this great love be inside me?

Look at your eyes. They are small,
but they see enormous things.

(Rumi)


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Invisibleanne halonium
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Registered: 05/07/13
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Re: Slant tubes question [Re: LoveLightPeace]
    #18715316 - 08/16/13 04:10 PM (10 years, 5 months ago)

the obvious answer here is culture bottles.



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InvisibleFrankHorrigan
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Re: Slant tubes question [Re: LoveLightPeace]
    #18718042 - 08/17/13 07:57 AM (10 years, 5 months ago)

For cubes, keep them in the fridge around 40F in an insulated lunchbox (to protect from temp swings).


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineLoveLightPeace
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Registered: 08/26/11
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Re: Slant tubes question [Re: FrankHorrigan]
    #18725804 - 08/19/13 02:13 AM (10 years, 5 months ago)

Thnx:)


--------------------
I am so small I can barely be seen.
How can this great love be inside me?

Look at your eyes. They are small,
but they see enormous things.

(Rumi)


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OfflineLoveLightPeace
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Registered: 08/26/11
Posts: 286
Last seen: 4 years, 11 months
Re: Slant tubes question [Re: RogerRabbit]
    #18806159 - 09/06/13 03:32 AM (10 years, 4 months ago)

Quote:

RogerRabbit said:
A centrifuge is a tool which spins at high speed to separate materials with different weights.

Use test tubes with at least a 25mm opening or it's too hard to get the cultures out.  I use 25mm X 125mm for short term use and 60mm X 250mm for long term storage.
RR




May i ask wat one shud use to cut/get cultures out of (25mm or larger) slants? A scalpel with a specific sized blade perhaps? Also forgive my ignorance but shudnt smaller sized slants be used for keeping cultures long term since there'll be less air space which i assume wud be more ideal for keeping cultures in long term? Im asking becuz u said: "I use 25mm X 125mm for short term use and 60mm X 250mm for long term storage."


--------------------
I am so small I can barely be seen.
How can this great love be inside me?

Look at your eyes. They are small,
but they see enormous things.

(Rumi)


Edited by LoveLightPeace (09/06/13 06:57 AM)


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