Home | Community | Message Board

Avalon Magic Plants
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Kratom Powder For Sale   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Grow Bags

Jump to first unread post Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Frank's agar media journal * 29
    #18676888 - 08/08/13 08:13 AM (10 years, 7 months ago)



I'm going to keep track of agar recipes here.

I'll be trying out a whole bunch of different recipes from Aloha medicinals' cookbook.

I'm looking to use most of these for cubensis as well as gourmets. Some are species-specific though, such as the PAT. All the pictures for this thread will be cubensis cultures.

A more in-depth version of this (across multiple species) will be added to my journal at some point.

If anyone is interested feel free to join in the thread, this is going to be an on-going (and sometimes slow) process but I plan to finish it by the end of this calender year.

This is not going to be an agar tutorial initially though maybe it will become one, please refrain from asking unrelated questions.

My first goal was to measure out the agar recipes and bag them up. So here we go...

Each recipe calls for 500mL of water (tap water used unless otherwise noted).

I'm probably omitting the peptone until I have a better supplier. It is expensive and not readily available for me at this time.


0) Grain-dunk agar experiment: 10g of agar to 500mL of water used to dunk 6 quarts of colonized WBS


Here are comparison plates between isolates on grain-dunk agar and malt-extract with yeast agar. I did not use very good sterile technique so the plates are a bit spotty here or there.  These are simply for a visual comparison, they are not going to grains.



Grain-dunk agar works ok, I would add some dextrose to the recipe if I try it again. Growth was pretty wispy compared to other media.

1) Malt-extract: 10g LME(a) / GLME (b), 10g agar


Pictured here is GLMEA, it works great, pretty much the standard I judge media against.


2) Malt-extract yeast: 10g ELME, 10g agar, 1g yeast
 


Vigorous growth. I use this regularly.


3) Malt-extract with activated carbon: 10g ELME, 10g agar, 1g activated carbon



Didn't notice much difference between standard LMEA. Not recommended.


4) Malt-extract with peptone: 10g LME, 10g agar, 1g peptone


5) Malt-extract with yeast and peptone: 10g LME, 10g agar, 1g yeast, 1g peptone


6) Malt-extract with yeast and peptone and activated carbon: 10g LME, 10g agar, 1g yeast, 1g peptone, 1g activated carbon



7) Malt-extract with millet: 10g LME, 10g agar, 10g millet flour



Growth was slower and fuzzier than I'd like. No reason to recommend.


8) 597: 10g agar, 3.75g LME, 0.3g yeast, 0.5g peptone



9) Malt-extract yeast agar with vitamins: 10g LME, 10g agar, 8g ground crickets, 1g yeast, 0.01g multivitamin



10) Malt-extract yeast agar with elements: 10g LME, 10g agar, 1g yeast, 0.3g CaMgZn


Growth was comparable to standard LMEA, not any reason to recommend adding vitamins to agar for cubes.


11) Blood worm agar: 10g agar, 5g dry blood worms



12) Krill agar: 10g agar, 10g dry krill



13) Fish flake dextrose agar: 10g agar, 3g fish flakes, 10g dextrose



This did ok. Interestingly, one of my cultures really loved the shit out of it (see above) and the rest performed better on LMEA.


14) Meal worm agar: 10g agar, 10g meal worms, 3g dextrose, 1g yeast


As you can see, cubes don't really thrive on MWA. Growth was slow and strange. I would not recommend it.


15) Cat food agar: 10g agar, 10g cat food



16) Bird seed malt agar: 10g agar, 2.5g BRF, 5g WBS, 2.5g LME, 0.5g peptone



17) Millet agar: 10g agar, 10g millet flour, optional: 1g yeast



18) Cornmeal dextrose agar: 10g agar, 25g cornmeal, 3g dextrose



19) BRF Malt-extract agar: 10g agar, 40g BRF, 2g malt, 1g yeast



20) Potato dextrose agar: 10g agar, 10g potato starch, 7g dextrose


21) PAT (cordyceps-specific): 10g agar, 100mL potato broth, 1g KH2PO4, 0.5g MgSO4, 1.5g peptone, 0.5g ammonium citrate, 15g dextrose, .025g thiamine hcl



22) Potato dextrose yeast agar: 10g agar, 10g potato starch, 7g dextrose, 1g yeast



Another de facto standard for media. This is my favorite recipe at the moment.


:cheers:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Edited by FrankHorrigan (02/07/14 05:36 PM)

Extras: Filter Print Post Top
OfflineSpencerPhillips
Male User Gallery


Registered: 08/18/12
Posts: 656
Loc: ON, Canada Flag
Last seen: 1 month, 14 hours
Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18676894 - 08/08/13 08:15 AM (10 years, 7 months ago)

was reading this in your journal which looked much similar, does the recipe book your taking from call for dry cat food?

sorry just wondering..

EDIT: #15


--------------------
Come on put it up! Woooo!

Edited by SpencerPhillips (08/08/13 08:16 AM)

Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Frank's agar adventures journal [Re: SpencerPhillips] * 1
    #18676898 - 08/08/13 08:17 AM (10 years, 7 months ago)

I am using dry cat food for it.

It will dissolve into a powder when hydrated.

I don't think wet food would have the right consistency, with the chunks and what not :shrug:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Extras: Filter Print Post Top
Invisibletbagtag
Boomer Barron

Registered: 01/16/13
Posts: 1,432
Loc: Amsterdam Flag
Re: Frank's agar adventures journal [Re: SpencerPhillips]
    #18676900 - 08/08/13 08:17 AM (10 years, 7 months ago)

:pedrophile:

Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Frank's agar adventures journal [Re: tbagtag]
    #18676909 - 08/08/13 08:21 AM (10 years, 7 months ago)

:lurker:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Extras: Filter Print Post Top
Invisibletbagtag
Boomer Barron

Registered: 01/16/13
Posts: 1,432
Loc: Amsterdam Flag
Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18677067 - 08/08/13 09:17 AM (10 years, 7 months ago)

Lol, I have nothing to add just wanted to keep this thread saved for more study.  Tomorrow marks one week since my second agar attempt.  Struggling like a mother fucker with it.

Extras: Filter Print Post Top
Offlinejonsnow
Stranger
 User Gallery

Registered: 07/22/13
Posts: 24
Last seen: 10 years, 7 months
Re: Frank's agar adventures journal [Re: tbagtag]
    #18677113 - 08/08/13 09:34 AM (10 years, 7 months ago)

are you using brewer's yeast or nutritional yeast?

Extras: Filter Print Post Top
InvisibleJuiceh
Dabbing All Day
 User Gallery


Registered: 09/25/12
Posts: 3,208
Re: Frank's agar adventures journal [Re: jonsnow]
    #18677311 - 08/08/13 10:28 AM (10 years, 7 months ago)

:rockon:  :takingnotes:
I Love Agar! I've only worked with MEA thus far. Was thinking of switching it up a little for the next batch. I can't wait to see your results!


--------------------
Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR

Extras: Filter Print Post Top
InvisibleVirginiaGentleman
Lignin seeker
 User Gallery


Registered: 07/09/11
Posts: 714
Loc: birch beech maple oak
Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18677612 - 08/08/13 11:49 AM (10 years, 7 months ago)

Quote:

FrankHorrigan said:


1ab) Malt-extract: 10g LME(a) / GLME (b), 10g agar

23) Wood-lovers agar: add 10g sawdust to agar recipe.






Been using (1ab), sometimes going .80LME:1agar with good results

Curious if the sawdust would suspend or settle for (23) though, seems like that would require a pour method which i'm not too fond of.. Maybe i'll give it a shot during the next transfer :takingnotes:

From what i can gather, newspaper pulp or egg carton pulp should have similar effect as sawdust by providing the lignin (i'm assuming) woodies are hungry for.


--------------------
Ps. Allenii, Arcana, Azure, Baeos, Caerulipes, Cyan, Ovoid, Subreg, etc FT - Only seeking Ps. woodlovers - legal USA + CAN - Intended for microscopy use only

Edited by VirginiaGentleman (08/08/13 11:51 AM)

Extras: Filter Print Post Top
OfflinePhosCap
Gratuitous Heavenly Grace
 User Gallery


Registered: 06/09/10
Posts: 975
Loc: Tartary
Last seen: 10 months, 10 days
Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18677635 - 08/08/13 11:53 AM (10 years, 7 months ago)

:popcorn:


--------------------
Psychoactive Fungi of the World        TLs            Franks teks and Tips        Stonesun's..
Spitballs Quick Rye Prep

Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Frank's agar adventures journal [Re: jonsnow]
    #18678367 - 08/08/13 02:08 PM (10 years, 7 months ago)

Quote:

VirginiaGentleman said:

Been using (1ab), sometimes going .80LME:1agar with good results

Curious if the sawdust would suspend or settle for (23) though, seems like that would require a pour method which i'm not too fond of.. Maybe i'll give it a shot during the next transfer :takingnotes:

From what i can gather, newspaper pulp or egg carton pulp should have similar effect as sawdust by providing the lignin (i'm assuming) woodies are hungry for.




Good stuff. I know that with most of these recipes I will need to swirl the agar to mix it every few plates poured. I've also heard water used to soak woodchips is good for making agar for wood lovers.

My lions mane is picky and slow on my MEA. I'm going to test this out soon.

Quote:

jonsnow said:
are you using brewer's yeast or nutritional yeast?




Nutritional :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Edited by FrankHorrigan (08/08/13 08:01 PM)

Extras: Filter Print Post Top
InvisibleZarotti
Stranger

Registered: 05/12/12
Posts: 314
Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18678690 - 08/08/13 03:13 PM (10 years, 7 months ago)

Is it true that the lower the nutrition value of the agar&malt mix
the more rhyzomorphic mycelium growth will appear?

And whats the difference inbetween brewer's yeast and nutritional yeast?
Does it matter?

Extras: Filter Print Post Top
InvisibleLustyLocks
I'm a teapot

Registered: 01/10/12
Posts: 1,513
Loc: Mom's basement Flag
Re: Frank's agar adventures journal [Re: Zarotti]
    #18678784 - 08/08/13 03:34 PM (10 years, 7 months ago)

Cat food. Meow!! :shockedpussy:
I might have to try that. It sounds interesting. I imagine there is yeast and other goodies in there?

. I'm looking forward to the upcoming posts in this thread.


--------------------

Extras: Filter Print Post Top
OfflineStromriderM
This must be the place
Male User Gallery


Registered: 06/02/13
Posts: 7,344
Loc: Dept of know what I'm say... Flag
Last seen: 18 hours, 10 seconds
Trusted Cultivator
Re: Frank's agar adventures journal [Re: LustyLocks]
    #18678899 - 08/08/13 03:55 PM (10 years, 7 months ago)

:takingnotes:

Extras: Filter Print Post Top
Offlinejustarandom
around here somewhere

Registered: 07/28/13
Posts: 67
Last seen: 1 year, 4 months
Re: Frank's agar adventures journal [Re: Stromrider]
    #18680546 - 08/08/13 09:37 PM (10 years, 7 months ago)

Quote:

psillyshroomer said:
:takingnotes:



agreed

Extras: Filter Print Post Top
Invisiblenucleophile
international cowboy chemist

Registered: 04/28/13
Posts: 299
Re: Frank's agar adventures journal [Re: justarandom]
    #18680776 - 08/08/13 10:26 PM (10 years, 7 months ago)

I'm curious to see how the cat food agar performs... I grew some oysters on a  cat food/sawdust/grain substrate once... they didn't much care for it but dutifully fruited as oysters are bound to do.

Extras: Filter Print Post Top
Offlinepsilocybebonsai
Not a Stranger
 User Gallery


Registered: 09/17/05
Posts: 379
Last seen: 3 months, 16 days
Re: Frank's agar adventures journal [Re: nucleophile]
    #18681391 - 08/09/13 02:17 AM (10 years, 7 months ago)

commenting for two reasons!

1. so i can come back and see what people are saying and;
2. is there a list as to the benefits of specific nute additives? (i understand sawdust for wood lovers, etc) but a more in depth so i can potentially experiment with my own would be great. for example, could i use straw juice with agar?

thanks ya'll


--------------------
My small photo collection here
My smaller grow log here
Some tips for taking photos of your progress when seeking help here

Extras: Filter Print Post Top
Offlinepsilocybebonsai
Not a Stranger
 User Gallery


Registered: 09/17/05
Posts: 379
Last seen: 3 months, 16 days
Re: Frank's agar adventures journal [Re: nucleophile]
    #18681415 - 08/09/13 02:28 AM (10 years, 7 months ago)

Quote:

nucleophile said:
I'm curious to see how the cat food agar performs... I grew some oysters on a  cat food/sawdust/grain substrate once... they didn't much care for it but dutifully fruited as oysters are bound to do.




i actually did some catfood agar with and oyster tissue clone and it worked very well. my catfood wasnt as powdery as i was hoping (used a blender, not a coffee grinder) and it performed really well (first agar, but i couldnt have expected anything better, really)


--------------------
My small photo collection here
My smaller grow log here
Some tips for taking photos of your progress when seeking help here

Extras: Filter Print Post Top
InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18681452 - 08/09/13 02:50 AM (10 years, 7 months ago)

Have you got a personal favorite and/or do you use different medias for different strains? If so, which is it and why? :smile:


--------------------


The Corbett Report
Open Source Intelligence News


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,830
Loc: Canada Flag
Trusted Cultivator
Re: Frank's agar adventures journal [Re: Pestile]
    #18681622 - 08/09/13 05:19 AM (10 years, 7 months ago)

:popcorn: :takingnotes:

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | Next >  [ show all ]

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Kratom Powder For Sale   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Grow Bags


Similar ThreadsPosterViewsRepliesLast post
* Best agar media recipe? shamantra 4,021 6 06/04/04 04:36 AM
by ATWAR
* Purchasing pre-mixed Agar media distortion 1,257 2 02/02/03 12:57 PM
by SmashedMe
* Benefits of Agar? celsius 9,457 10 12/01/19 12:36 AM
by wildernessjunkie
* AGAR AND STUFF......LOADS OFF QUESTIONS......LOL smokes 1,679 1 11/17/02 12:07 PM
by Zero7a1
* 9 different strains...starting multispore on agar.... Horseman 2,194 3 08/11/03 12:18 PM
by Twista
* Agar and growing that white stuff! pj541 2,553 5 10/15/01 04:06 PM
by pj541
* Agar Questions cantara 5,236 6 08/09/01 06:22 AM
by ar393
* Media Antibiotic opioq 1,076 5 06/16/05 01:59 PM
by Roadkill

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
128,289 topic views. 22 members, 141 guests and 81 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.007 seconds on 14 queries.