Home | Community | Message Board


FreeSpores.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Papaver Somniferum, Petri Dish, Wild Bird Seed

Jump to first unread post. Pages: 1 | 2 | Next >  [ show all ]
Anonymous

Post deleted by Papaver
    #1865766 - 08/30/03 02:41 PM (13 years, 3 months ago)



Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSuperbowl Bob
member
Registered: 08/09/03
Posts: 166
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: ]
    #1865773 - 08/30/03 02:44 PM (13 years, 3 months ago)

How's your jar sterilization method?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: ]
    #1865774 - 08/30/03 02:44 PM (13 years, 3 months ago)

What substrate are you using and how are you filtering your jars?


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblebotanisthype
P$ylocibe$ounDW@vE!
Male User Gallery

Registered: 07/31/03
Posts: 839
Loc: DECEPTICON MANOR
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: dog]
    #1865788 - 08/30/03 02:50 PM (13 years, 3 months ago)

wait u said u shkook the jars...after inoculation?you should do that >.it air thats gettin in ..like if YOu have you lid rubber side up...when u fill it loose,the top can make a pop noise,and send a slight current of air pressure that can suck in that 1 germ!..i dont shake em....pat a few times preparing jars.and i leave em with the rest of the family!


--------------------
Please +REP if i've been of any help... thanks!!!

Dinosaur of the Funny Family, though I have short arms, they fit in my GB... lol


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHaveABeer420
G.O.D.
Male

Registered: 04/29/03
Posts: 666
Loc: The Carnival of Souls
Last seen: 1 year, 29 days
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: botanisthype]
    #1865804 - 08/30/03 02:59 PM (13 years, 3 months ago)

Maybe your syringes are contam'd.  :smirk: 


--------------------
I don't charge by the inch I charge by the foot, think I'm lying bitch here take a look...100% U.S.D.A grade A beef, heres my card call me.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinepsilocybinjunkieM
relaxin
Male

Registered: 03/18/01
Posts: 13,338
Last seen: 4 hours, 34 minutes
Trusted Cultivator
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: ]
    #1865837 - 08/30/03 03:15 PM (13 years, 3 months ago)

If your not cleaning your jars with a bleach solution after contamination hit your always gonna have contams! Use 30/70 bleach to water to sterilize your old jars then put in dishwasher. Jars should be sterilized for at least 12 hours in bleach solution before you wash them...
A friend was having the same prob as you years ago and this was the cause~
I u use bleach your prob will go away most likely :stash:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSubGen1us
 User Gallery

Registered: 11/26/02
Posts: 3,427
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: ]
    #1865840 - 08/30/03 03:18 PM (13 years, 3 months ago)

ur karo got messed up when u injected peroxide into it to kill contams but saddly the mushroom spores will also get killed because all contams are is foreign spores...


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineresin
Ghetto Monster
Male User Gallery

Registered: 06/12/02
Posts: 2,815
Loc: Ca$hville
Last seen: 20 days, 10 hours
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: SubGen1us]
    #1865856 - 08/30/03 03:25 PM (13 years, 3 months ago)

Also bleach your lids / filter disks. I use popcorn, am dirty as fuck, and havent had a single contamn in the jar


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Post deleted by Papaver [Re: psilocybinjunkie]
    #1865917 - 08/30/03 03:57 PM (13 years, 3 months ago)



Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: ]
    #1865935 - 08/30/03 04:05 PM (13 years, 3 months ago)

If your jars are properly sealed and filtered (and I assume they are) then the contamination is not being introduced by shaking. The contams were already there - shaking just spread them around and increased their rate of growth.

Were you having these problems before using the Karo solution? If not then that is your culprit. It can be difficult to spot contams in mycelium solutions, which is why my friend does not use them. A lot of jars can be lost very quickly.

If you were having these problems prior to using the Karo then you need to double check your syringe making and/or inoculation techniques.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Post deleted by Papaver [Re: dog]
    #1865981 - 08/30/03 04:23 PM (13 years, 3 months ago)



Post Extras: Print Post  Remind Me! Notify Moderator
Offlineresin
Ghetto Monster
Male User Gallery

Registered: 06/12/02
Posts: 2,815
Loc: Ca$hville
Last seen: 20 days, 10 hours
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: ]
    #1866032 - 08/30/03 04:45 PM (13 years, 3 months ago)

Think nigger, the jars arent the prob, then it must be the spores or your innoculation technique. I innoculate by unscrewing the lid, and squirting the spores in, without puncturing the filter disk, and have never had contamns. Its either the spores, or your technique


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: ]
    #1866068 - 08/30/03 04:56 PM (13 years, 3 months ago)

if you germinate the spores in kero, it is already too late to add h2o2 .chances are any trich. spores in there have already germinated too .
try germinating on a small piece of sterilized grey cardboard or egg carton .
when the spores hatch you'll see lil'tiny white specks .
then add to kero water . 1cc of 3% h2o2 to 100ml karo water .
i haven't tried this using a contaminated syringe ,but it works well for wild prints .
good luck . let use know how it goes .


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: ]
    #1866082 - 08/30/03 05:00 PM (13 years, 3 months ago)

Peroxide kills spores effectively. Peroxide does not kill contaminant mycelium effectively.

You need new spores. Even better invest in some Media and petris, and never have to be unsure about the inoculant.


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Post deleted by Papaver [Re: ]
    #1866231 - 08/30/03 05:34 PM (13 years, 3 months ago)



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: ]
    #1872003 - 09/01/03 07:54 PM (13 years, 3 months ago)

I have never used Karo before but if it works for liquid culture it should work for agar culture.
Probably could leave out the Potatoe as well.

I use Malt and supplement every once in awhile if they are older cultures.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: ]
    #1872042 - 09/01/03 08:13 PM (13 years, 3 months ago)

Paradis...
Liquid inoculations = all or nothing method
You should test it before inoculating dozens of jars.

Test it on a jar or two, if they become contaminated, throw them away, don't waste time with h2o2.

Shaking the jars after the inoculation is a wise thing to do.

Even if the karo/honey/whatever media looks good, you cannot see bacteria in the water. Same as with molds.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblepsilomonkey
Twisted brainwrong of a oneoff man mental

Registered: 08/08/03
Posts: 812
Loc: Airstrip One
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: MushMushi]
    #1873678 - 09/02/03 10:38 AM (13 years, 3 months ago)

You are using bird seed. I have read that some grains can contain contam spores deep in the grain kernel, and therefore are a bugger to get out, they are protected by the grain during sterilization and germinate before they come into contact with the h2o2 and may have already started producing h2o2 destroying enzymes.

One method is fractional sterilzation. I would give the seed a quick simmer 10mins or so, then let it stand over night to let the spores protected inside the grain to germinate and come out of their little spore castle, the bash it with the PC thing, continue as normal.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleOJK
Stranger
 User Gallery
Registered: 06/08/03
Posts: 10,629
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: psilomonkey]
    #1873698 - 09/02/03 10:55 AM (13 years, 3 months ago)

Quote:

took a q-tip, dipped it into rubbing alcohol then put a drop of alcohol onto the spot where i was gonna innoculate. The I took an alcohol soaked paper towel and wiped off the needle then stuck it in and inoculated.Then I took another alcohol soaked rag and put it over the needle and withdrew the needle.Then I quickly lifted the paper towel and put on a piece of tape over the tiny tiny hole in the filter disc.




You need to flame sterilize your needle until it glows red hot before innoculating, alcohol is used simply as a sterile way to cool it down. I doubt this is the source of your contamination problems, but it's worth bearing in mind.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblepsilomonkey
Twisted brainwrong of a oneoff man mental

Registered: 08/08/03
Posts: 812
Loc: Airstrip One
Re: FUUUUUUUUUUUUUUUUUUUCKKKKKKKKKKK! [Re: OJK]
    #1874414 - 09/02/03 04:26 PM (13 years, 3 months ago)

Oh.. and if you want to take the scientific approch, try cooking up a jar but not innoculating, if it gets contam its the grain, if not its something else..


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1 | 2 | Next >  [ show all ]

Amazon Shop for: ½ Pint Jars, Agar, Papaver Somniferum, Petri Dish, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Cambo's anyone that has tried them please post Anonymous 1,561 8 04/11/03 05:48 PM
by shirley knott
* post *DELETED* kmoses2 743 11 01/30/09 11:51 PM
by SethTheGreat
* post *DELETED* kmoses2 538 5 04/04/09 04:46 PM
by Frequency
* post *DELETED* kmoses2 801 7 04/16/09 01:22 AM
by RogerRabbit
* post *DELETED* kmoses2 704 5 04/29/09 12:19 AM
by RogerRabbit
* Mexican Tapalpa prints...not very good
( 1 2 all )
ralphster44 4,632 37 02/03/02 06:47 PM
by Zen Peddler
* The SHROOMERY RAFFLE IS HERE!*update*NEW PRIZES!!
( 1 2 3 4 5 all )
ThorA 12,974 84 03/04/02 09:42 AM
by Anno
* 4 Day jars
( 1 2 all )
goolag 2,929 23 05/30/02 01:53 PM
by luvdemshrooms

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
1,445 topic views. 21 members, 55 guests and 26 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
High Mountain Compost
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.062 seconds spending 0.005 seconds on 16 queries.