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OfflineChopperDave
member
Registered: 08/10/03
Posts: 177
Last seen: 12 years, 8 months
Adding sterile water to pre-made syringe?
    #1860173 - 08/28/03 08:27 PM (13 years, 3 months ago)

A foaf got a premade syringe, and he was wondering if he could boil water, wait until it cooled, and then draw it up into the syrine, to increase number of innoculations? He realizes it might take longer, as the spores would be spread out...hes only asking because he has 1cc left and would like to try a honey/water jar.


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Invisiblefungusflip
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Registered: 12/08/02
Posts: 284
Loc: texas
Re: Adding sterile water to pre-made syringe? [Re: ChopperDave]
    #1860195 - 08/28/03 08:33 PM (13 years, 3 months ago)

heat up the end of the needle, poke it right through the side of an un-opened jug of DISTILLED water and suck up however much you think you need. distilled water is sterile.


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let it be know, there is a fountain that was not made by the hands of men.


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OfflineBatman
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Registered: 07/21/03
Posts: 79
Loc: Gotham City
Last seen: 10 years, 7 months
Re: Adding sterile water to pre-made syringe? [Re: fungusflip]
    #1860235 - 08/28/03 08:45 PM (13 years, 3 months ago)

I really dont think thats a good idea. i think u should boil the water for atleast 10 mins let it cool with the lid on it and then suck up that water... better safe then sorry. :laugh:


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Invisiblebotanisthype
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Registered: 07/31/03
Posts: 839
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Re: Adding sterile water to pre-made syringe? [Re: Batman]
    #1860446 - 08/28/03 10:10 PM (13 years, 3 months ago)

try karo tek..sterilize it ...let cool and .suck up the mixture with your remaining cc and let sit in incubation..i just did that with my malabars ..but i think the sporesare not viable..but hey thats the only way i would find out!


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Invisiblear393
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Registered: 01/27/00
Posts: 696
Loc: NYC, NY, US
Re: Adding sterile water to pre-made syringe? [Re: botanisthype]
    #1860455 - 08/28/03 10:14 PM (13 years, 3 months ago)

if you are going to go honey water/Karo, that one cc is more than you need to innoculate the liquid media. a few drops of the syringe would work if the spores are distributed. (and with one cc left they should be)
-ar393


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OfflineBatman
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Registered: 07/21/03
Posts: 79
Loc: Gotham City
Last seen: 10 years, 7 months
Re: Adding sterile water to pre-made syringe? [Re: ar393]
    #1860564 - 08/28/03 10:46 PM (13 years, 3 months ago)

that might work also! :laugh:


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OfflineDemiurge
creator ofworlds

Registered: 04/11/03
Posts: 693
Loc: Florida
Last seen: 5 years, 8 months
Re: Adding sterile water to pre-made syringe? [Re: Batman]
    #1860805 - 08/28/03 11:46 PM (13 years, 3 months ago)

I'll second the distilled water. It is sterile. If you open up the jug to boil the water, then you are increasing your chances of contamination. Just sticking the syring into the side of the jug would be the best idea. Just wie off the spot on the outside with alcohol before you stick it.


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Just another animal embeded in a technological coral reef extruded psychic objects...


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