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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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steam sterilization, what can and can not do
    #18572827 -

So I got the plans laid out and gathered mostly everything.... Except the pressure cooker.

Can I steam sterilize quart jars of rye berries? A bottle of agar to be poured onto Petri dishes?

I got myself a 50 lb bag and now  I just want to get a bunch of jars going.

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Re: steam sterilization, what can and can not do [Re: blojo02184]
    #18572832 -

blojo02184 said:
So I got the plans laid out and gathered mostly everything.... Except the pressure cooker.

Can I steam sterilize quart jars of rye berries? A bottle of agar to be poured onto Petri dishes?

I got myself a 50 lb bag and now  I just want to get a bunch of jars going.


Pressure cooker.


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Re: steam sterilization, what can and can not do [Re: iSmkGrnBud]
    #18572874 -

8 fukn dreadfull hrs and you will have sterile berries!! Its a major pita but works. Keep filling the pot up every 30 min with boiling water to keep the temp up. Probably be off making a shit load of brf jar and spawning to bulk. Only 90 min of steaming for those. And you could do 3 pots full of jars in the same amount of time.

Yes, get a pc yesterday!!


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Re: steam sterilization, what can and can not do [Re: first time expert]
    #18572906 -

True.... Very true first time...

My only problem is im not getting any pressure cooker.... Im looking for an aa 921 or 930.
And that type of money doesn't grow on trees.

Edited by blojo02184 (07/18/13 07:42 AM)

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Re: steam sterilization, what can and can not do [Re: blojo02184]
    #18573000 -

Lets do some math :smile:


Buy Pressure cooker from wallie world - $40-65

Sterilize Jars upon Jars of berries - $cost of rye + jars and water

Fruit those lovely things - $cost of pasteurizing sub

Sell the lovely edibles you have just harvested for your money back - Priceless


It's like a sweet investment that you'll never regret.


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Re: steam sterilization, what can and can not do [Re: Ghost4]
    #18573052 -

I love math....Im very good at it when It comes to selling fruits and flowers!! But yea a cheep pc will pay for it self in a month when you have 30 qts. of spawn!! Im still looking for a pc and have been putting my money into some thing else...but a pc is next to come!!


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Re: steam sterilization, what can and can not do [Re: first time expert]
    #18573146 -

Yeah I feel ya.
Right now nutes for my garden are first. I also checked Wally world... No pressure cookers? Or at least the ones near me.

Jars $30 + rye berries 50lbs @ $43 + nutes $30-$40 + pressure cooker $80 (Amazon presto 23 qt) - $280

PC is last.... I just hear so many good things about AA pc's I just want one. Presto any good?

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Re: steam sterilization, what can and can not do [Re: blojo02184]
    #18573334 -

it's roughly the price of a quarter of good weed in the US!!!


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Re: steam sterilization, what can and can not do [Re: Ghost4]
    #18573742 -

Fractional Sterilisation


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Fungi nutrition requirements

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Re: steam sterilization, what can and can not do [Re: Subspecies]
    #18573759 -

Subspecies said:
Fractional Sterilisation


^ This fails 90% of the time.


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: steam sterilization, what can and can not do [Re: PussyFart]
    #18573771 -

NAH, are you depressed today?


Shittin' on people


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Re: steam sterilization, what can and can not do [Re: Ghost4]
    #18573782 -

Don't take it personal, it's the truth.

Fractional sterilization/tyndilization fails a majority of the time.

Either steam sterilize grains for 8 hours, or PC them @ 15psi for 90 minutes.

A simple search will result the same response from many TCs.


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: steam sterilization, what can and can not do [Re: PussyFart]
    #18573972 -

Notahacker420 said:
Subspecies said:
Fractional Sterilisation


^ This fails 90% of the time.


Thats a shame. Pressure cookers are very uncommon here(hardly anyone has cooked with them for decades), and the ones you can get are small and expensive, not suitable for doing multiple or tall jars. I plan on trying a combination of tyndallisation/peroxide. Rather than simply letting all the spores germinate in between each boiling phase, I plan to add peroxide to kill spores before they can germinate. If there is excess peroxide it will be decomposed by the next boiling phase. The enzymes present in the substrate that would decompose the peroxide are destroyed at 60C.

I do have a proper autoclave, but it is not suitable either. The chamber is small and the shape of a can turned on its side. Perfect for instruments, not for jars.


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Re: steam sterilization, what can and can not do [Re: Subspecies]
    #18573996 -

.... you don't cook with pressure cookers, you seal jars filled with anything and everything.


You can cook with them... but it's less reasonable than canning


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Re: steam sterilization, what can and can not do [Re: Subspecies]
    #18574103 -

Subspecies said:
Notahacker420 said:
Subspecies said:
Fractional Sterilisation


^ This fails 90% of the time.


Thats a shame. Pressure cookers are very uncommon here(hardly anyone has cooked with them for decades), and the ones you can get are small and expensive, not suitable for doing multiple or tall jars. I plan on trying a combination of tyndallisation/peroxide. Rather than simply letting all the spores germinate in between each boiling phase, I plan to add peroxide to kill spores before they can germinate. If there is excess peroxide it will be decomposed by the next boiling phase. The enzymes present in the substrate that would decompose the peroxide are destroyed at 60C.

I do have a proper autoclave, but it is not suitable either. The chamber is small and the shape of a can turned on its side. Perfect for instruments, not for jars.


This will fail too.  It's best to listen to those who have come before.  Either pressure cook grains, or steam for 8 hours.  Peroxide isn't going to cut it for grains.

For the record, any more talk of dealing in this thread is going to result in some ban points handed out. . .
RR


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Re: steam sterilization, what can and can not do [Re: RogerRabbit]
    #18574129 -

Sorry forgot to mention, I'm not cooking grains. I'm doing agar. DO you still expect this will fail?


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Re: steam sterilization, what can and can not do [Re: Subspecies]
    #18574134 -

Subspecies said:
Sorry forgot to mention, I'm not cooking grains. I'm doing agar. DO you still expect this will fail?


Oh my god, yes it will.

Order a PC online :thumbup:


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Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: steam sterilization, what can and can not do [Re: FrankHorrigan]
    #18574167 -

Are you saying peroxide agar does not work? Or that I will fail? Is there a reason other than anecdotal evidence? Because if peroxide works to prevent spore germination on agar, and there are no live bodies left, there is no reason it should fail that I can see?

Anyway I'm putting the finishing touches on on my laminar flow cabinet/positive pressure glove box. Delivery of a PC and jars will take time. I have raw material to clone from, so I will probably try regardless of expectations :smile:


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Re: steam sterilization, what can and can not do [Re: Subspecies]
    #18574179 -

You need to PC agar, no questions about it.

It contams just as or even more readily than your grains.

If you want to try, have at it. I'm surprised you have access to all this stuff you speak of but you don't have a PC :shrug:


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Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: steam sterilization, what can and can not do [Re: FrankHorrigan]
    #18574206 -

Like I mentioned, people haven't cooked or preserved here with PCs for decades, I'm not in the USA. It is only in recent years they have started popping up in stores again, but they are all tiny, not canning cookers. I know agar contams easily, I've used it in the past. I've also read a lot of people using agar in non sterile environments with peroxide so I'm going to give it a go. The theory is sound. If you are starting from spores, then peroxide will not work, but cloning tissue is another matter.

My friend always had success without a PC and the original PF-Tek. Very few contams.


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