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InvisibleCitric
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Registered: 03/19/04
Posts: 4,490
Trusted Cultivator
Post deleted by Anno [Re: Tomandjerry58]
    #2879146 - 07/11/04 06:16 PM (19 years, 6 months ago)



--------------------
Self Healing lid tek ** Update 10.17.17 **
Mini casing pictures: Pins to harvest
Cup O' Shrooms
Magash:  I noticed my contams were in the shape of fingers :whack:
Hyphae:  Yes  "Loss of moisture from the substrate"  is not a casing trigger.  :cuckoo:

My final Grow!


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InvisibleTheHateCamel
Research &Development -DBK
Registered: 01/31/03
Posts: 15,738
Re: post your advanced setup *DELETED* [Re: Citric]
    #2879643 - 07/11/04 09:26 PM (19 years, 6 months ago)

Post deleted by TheHateCamel

Reason for deletion: hj



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InvisibleCitric
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Registered: 03/19/04
Posts: 4,490
Trusted Cultivator
Post deleted by Anno [Re: TheHateCamel]
    #2884563 - 07/13/04 12:04 AM (19 years, 6 months ago)



--------------------
Self Healing lid tek ** Update 10.17.17 **
Mini casing pictures: Pins to harvest
Cup O' Shrooms
Magash:  I noticed my contams were in the shape of fingers :whack:
Hyphae:  Yes  "Loss of moisture from the substrate"  is not a casing trigger.  :cuckoo:

My final Grow!


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OfflineKaptKid
Spaced Pirate
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Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 3 years, 11 months
Re: post your advanced setup [Re: Citric]
    #2889506 - 07/14/04 12:23 PM (19 years, 6 months ago)

I shouldn't be smokin while typen


--------------------
Child of the 60's, Tripping ever since.


:sun:


Edited by KaptKid (07/14/04 12:32 PM)


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: post your advanced setup [Re: poke smot!]
    #2909342 - 07/20/04 04:28 PM (19 years, 6 months ago)

Ok, here's our advanced and automated inoculation method:

An agar culture is transferred to a sterilised blender with sterile water.


The blender is turned on and the agar culture is fragmented into tiny particles.


This mycelium broth is diluted over two bottles with sterilise water.


This is the peristaltic pump that will be used to pump to liquid inoculum into the bags. On top is a device we built, ourselves...


...It is a timer with a foot switch, so that the pump will deliver the same amount every time the pedal is pressed :laugh:


A silicone tube is fitted with a half syringe that is screwed tight. The tube itself is sterilised in the pressure cooker, The bottle with lliquified mycelium is in the flow cabinet, the tube goes into the bottle, then to the pump, then to the needle.


The bags are first soaked with alcohol, and left to stand for a few minutes. They are then wiped dry with a tissue and the needle is introduced. The footpedal is pressed, and presto, 10-100 ml is squirted in.


The bags are sealed with a hotglue gun.


Growth is fast.


The end result, healthy spawn!


I also use this setup to fill tubes with agar for my tissue culture plants.


--------------------
www.911blogger.com


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OfflineSHR00MiN
PSYCH0PSiL0CYBiN

Registered: 07/24/04
Posts: 456
Loc: RI
Last seen: 18 years, 5 months
Re: post your advanced setup [Re: comario2]
    #2944295 - 07/30/04 11:02 AM (19 years, 6 months ago)

wow, that looks like it goes right into the tub, pure genius!

but, how do you take showers?


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Offlineragadinks
MrBeatle
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Registered: 10/20/03
Posts: 1,298
Last seen: 3 months, 20 days
Re: post your advanced setup [Re: Una]
    #2944510 - 07/30/04 12:11 PM (19 years, 6 months ago)

Una: Nice tek - thanks for posting.

How many of the bags are you inocultating this way during a day?


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: post your advanced setup [Re: ragadinks]
    #2951119 - 08/01/04 06:10 AM (19 years, 5 months ago)

  >> How many of the bags are you inocultating this way during a day?

We can do about 100 bags in an hour using this method. Contamination is practically down to zero :laugh:


--------------------
www.911blogger.com


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OfflineTripleB
Hobbyist
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Registered: 04/01/04
Posts: 1,358
Loc: Michigan
Last seen: 9 years, 5 months
Re: post your advanced setup [Re: poke smot!]
    #2951230 - 08/01/04 09:05 AM (19 years, 5 months ago)

hey poke-smot, i like that setup u posted ...with the labeled casings and the nice looking stackers/shelevs... do tell about the setup more please?!?
maybe list the materials? and were to get them? thanx dude...


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Offlineragadinks
MrBeatle
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Registered: 10/20/03
Posts: 1,298
Last seen: 3 months, 20 days
Re: post your advanced setup [Re: Una]
    #2954889 - 08/02/04 02:03 AM (19 years, 5 months ago)

> We can do about 100 bags in an hour using this method. Contamination is practically down to zero.

Woow, that sounds good (and very professional) !
How do you sterilize so many bags at once ?


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OfflineCubieman420
Sharing in thegroove
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Registered: 03/20/02
Posts: 2,693
Loc: Midwest
Last seen: 10 years, 1 month
Re: post your advanced setup [Re: ragadinks]
    #2969496 - 08/05/04 07:15 PM (19 years, 5 months ago)

Probably an autoclave


--------------------
"...now waters run free, no more fish in the sea..."
1983-2004


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: post your advanced setup [Re: ragadinks]
    #2978829 - 08/08/04 04:51 PM (19 years, 5 months ago)

Actually we have three #925 cookers so we could sterilise 30 of these bags in day (that's two batches). If you save them up from a few days you would have a nice batch.


--------------------
www.911blogger.com


Edited by Una (08/08/04 04:53 PM)


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Offlinesplifner180
Student
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Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
Last seen: 1 year, 3 months
Re: post your advanced setup [Re: Una]
    #5494340 - 04/08/06 07:48 PM (17 years, 9 months ago)

Geek bump.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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OfflinehIgHdRoLoVeR
delete this user
Male
Registered: 03/25/06
Posts: 203
Last seen: 16 years, 3 months
Re: post your advanced setup [Re: poke smot!]
    #5496422 - 04/09/06 12:26 PM (17 years, 9 months ago)

very nice setup you have there poke smot!
however, for me its a matter of very basic math.
extra effort to set it up + me being lazy = fucking forget about it


--------------------
hIgHdRoLoVeR
Loving all things hydro since 1989.
http://www.gardenscure.com/420/index.php
the place to go for (medical) marijuana cultivation advice
World of Warcrack...My anti-job!


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OfflineSuperMario22
Shroom Fan
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Registered: 08/14/06
Posts: 27
Loc: USA, Earth, Milkyway
Last seen: 16 years, 5 months
Lego Mindstorm? [Re: hIgHdRoLoVeR]
    #5970060 - 08/16/06 04:41 PM (17 years, 5 months ago)

I just did a little research and found that you can get mindstorm sensors to monitor these very conditions:

Humidity Sensor - $149.00
http://www.legoeducation.com/store/detail.aspx?ID=380

LEGO-DCP Sensor Adapter - $59.00
http://www.legoeducation.com/store/detail.aspx?ID=380

9-Volt Temperature Sensor - $59.00
http://www.legoeducation.com/store/detail.aspx?KeyWords=temp&by=20&ID=324

It's not cheap, but it could work!

-Empyrean


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OfflineHippieChick
Chicks can do it too!
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Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 13 days
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Re: Lego Mindstorm? [Re: SuperMario22]
    #5980832 - 08/20/06 03:09 AM (17 years, 5 months ago)

Yes , but could you get them 3 years ago when this post was made,lol.

Peace,Love,Happiness and Harmony
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Lego Mindstorm? [Re: HippieChick]
    #5981271 - 08/20/06 11:45 AM (17 years, 5 months ago)

Quote:

HippieChick said:

Yes , but could you get them 2 years ago when this post was made,lol.






lmfao sis!~

no doubt!~

:grin:


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineTrippinOnAcid
Stranger
Registered: 08/03/06
Posts: 226
Last seen: 14 years, 1 month
Re: Lego Mindstorm? [Re: Roadkill]
    #5993158 - 08/24/06 04:37 PM (17 years, 5 months ago)

http://www.shroomery.org/forums/files/080503-46/2747-2bins.jpg

Could you tell me a little bit more about this set-up?

I get the idea of a basic tub in tub incubator but what are you using for airflow? I'm guessing the plumbing elbow has a computer fan attached to it and that the intake but weres the out take and what are you using for humidity?


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Offlinejagerbomber444
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Registered: 03/20/09
Posts: 166
Last seen: 13 years, 1 month
Re: post your advanced setup [Re: poke smot!]
    #10036716 - 03/25/09 12:49 AM (14 years, 10 months ago)

ive been researching for the last like month religously studying lol i've watched probably a hundred hours or more of videos and read at least like 6 hours a day on here every little bit of info. i can get my hands on well. i noticed you seem to be knowledgable about terrarium set ups i built mine out of an old dresser reinforced it ust a tarp to make it water tight so no water can get to the wood i beveled the tarp on top for condensation run off and mad a kind of reservoir out of the room on the bottom of the dresser i also sealed in two of the bottom rollers so i could put a drawer in. i used tarp to make that water tight with silicone sealant im gonna use that to put my perlite and casings in ive got the bubble stone set up with the air pump mounted outside of the terrarium in a filter box. i used another top to make a flap to cover the front with a plexi window for light to get in and so i could see the little guys growing :-) my question is do you think this should work and i cant seem to get the the temperature up high enough it settles at like 68 degrees when its empty just sitting any suggestions would be welcome.


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InvisibleGerman Kahuna
Facepalmer of Stoopid
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Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
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Re: post your advanced setup [Re: jagerbomber444]
    #10037132 - 03/25/09 05:06 AM (14 years, 10 months ago)

You are answering him on something he posted over 5 years ago. :rolleyes:


--------------------
"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".


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