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Callampin
Psyco Myco
Registered: 08/14/03
Posts: 520
Last seen: 6 months, 19 days
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H2O2 Sterilizing
#1854405 - 08/27/03 10:23 AM (20 years, 7 months ago) |
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hi, 2 out of the 3 times ive inoculated and done the whole proces ive gotten ALOT of contams, so im really intrested in knowing how to clean the wqhole work area plus the jars and everything , so i was wondering if its posible that by spraying everything with h2o2 it will be contam free at least for the time while im working , and also wanted to know if the h2o2 harms de mycelium or any of the shroom related stuff
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gldtn
Stranger
Registered: 02/24/03
Posts: 5
Last seen: 18 years, 10 months
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Re: H2O2 Sterilizing [Re: Callampin]
#1854434 - 08/27/03 10:40 AM (20 years, 7 months ago) |
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From my knowledge H2O2 usually harms bacterias and the mycelium, and it doesn't necessarly kills it. Have you tried spraying Lysol in the area your are working on? how about a glove box?
I hope that helps
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soochi
Chef
Registered: 08/13/02
Posts: 2,420
Loc: The Richest County
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Re: H2O2 Sterilizing [Re: Callampin]
#1854442 - 08/27/03 10:44 AM (20 years, 7 months ago) |
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use a more powerful surface sterilizer like lysol for tabletops, etc. spray the air with Oust. H202 doesn't adversly affect mycelium if it isn't too strong, the usual diluted h202 solution should be good enough to sterilize the insides of jars, utensils, containers, etc where growing substrate will come in contact. if myceila is introduced to a fairly high h202 enviroment it will slow down growth and adjust to the changed conditions, so again, use a diluted solution.
-------------------- Wee, sleeket, cowran, tim'rous beastie, O, what panic's in thy breastie! Thou need na start awa sae hasty, Wi' bickering brattle!
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psilomonkey
Twisted brainwrong of a oneoff man mental
Registered: 08/08/03
Posts: 812
Loc: Airstrip One
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Re: H2O2 Sterilizing [Re: soochi]
#1854495 - 08/27/03 11:03 AM (20 years, 7 months ago) |
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h2o2 is great at keeping down the nasties, but as soochi says it won't take out established contams. I use about 5ml of 3% h2o2 in a liter of water.
h2o2 will kill spores, but you can add it (best diluted and sprayed on) after the spores have germinated and mycelium is visible
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cyndyl
mycologist wannabe
Registered: 05/26/03
Posts: 193
Loc: far away from home
Last seen: 13 years, 2 months
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I wipe work areas with those lysol towelettes and sometimes the cholox towelettes. I then spray everything with 91%ISA. I only use the H202 to mix in small amounts w/h20 to keep it contaim free for misting and in the bottom of the growth chamber. remember to flame sterilize the syringe and wipe with alcohol. I once got some contams from not wiping the syringe all the way up to the plastic part. You could see the mold grow from the top of the innoculation points. hope that helps, its just IMO stuff...
-------------------- There's no such thing as a stupid question, but they're the easiest to answer!
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Callampin
Psyco Myco
Registered: 08/14/03
Posts: 520
Last seen: 6 months, 19 days
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Re: H2O2 Sterilizing [Re: soochi]
#1854523 - 08/27/03 11:11 AM (20 years, 7 months ago) |
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uhmm , ok , the only problem is that where i live they dont sell lysol , or at least not with that name , so im wondering about an equivalent do you know anything like it?
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psilomonkey
Twisted brainwrong of a oneoff man mental
Registered: 08/08/03
Posts: 812
Loc: Airstrip One
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Re: H2O2 Sterilizing [Re: Callampin]
#1854538 - 08/27/03 11:17 AM (20 years, 7 months ago) |
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I use surgical spirit on a paper towel to wipe down my work area, seems to work. I pressure cook the jars for 70mins (I use rye grain)
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acidhead1279
Master Of AllThings Hip
Registered: 03/01/03
Posts: 532
Loc: somewhere on the other si...
Last seen: 7 years, 2 days
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dude just mix some isoprophyl alcohol w/ water in a spray bottle and there you go. and either check you kitchen for a flow hood or get a hepa, or both oh yah, use OUST likr soochi said
-------------------- Farewell FRSE! We had such good times together.
Edited by acidhead1279 (08/27/03 11:30 AM)
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Callampin
Psyco Myco
Registered: 08/14/03
Posts: 520
Last seen: 6 months, 19 days
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mm ok i didnt know bout the isopropyl, im going for that one right now , thx
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Callampin
Psyco Myco
Registered: 08/14/03
Posts: 520
Last seen: 6 months, 19 days
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Re: H2O2 Sterilizing [Re: Callampin]
#1854646 - 08/27/03 11:45 AM (20 years, 7 months ago) |
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uhm , one question , whats a flowhood? lookit up everywere and still dont know T_T
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 20 hours
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Re: H2O2 Sterilizing [Re: Callampin]
#1854716 - 08/27/03 12:03 PM (20 years, 7 months ago) |
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Callampin
Psyco Myco
Registered: 08/14/03
Posts: 520
Last seen: 6 months, 19 days
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Re: H2O2 Sterilizing [Re: Anno]
#1854791 - 08/27/03 12:25 PM (20 years, 7 months ago) |
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thankx !
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Anonymous
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Re: H2O2 Sterilizing [Re: Callampin]
#1855939 - 08/27/03 06:09 PM (20 years, 7 months ago) |
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I would use rubbing alcohol for wiping shit down and spray the air with lysol....I've had very good success with this mix....i also agree with soochi and psilomonkey concerning h202...its a great preventitive but it won't do much once you got a contam
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Sev
Astropath
Registered: 06/06/03
Posts: 1,426
Loc: NY
Last seen: 9 years, 5 months
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Re: H2O2 Sterilizing [Re: Callampin]
#1856538 - 08/27/03 08:54 PM (20 years, 7 months ago) |
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Quote:
Callampin said: hi, 2 out of the 3 times ive inoculated and done the whole proces ive gotten ALOT of contams, so im really intrested in knowing how to clean the wqhole work area plus the jars and everything , so i was wondering if its posible that by spraying everything with h2o2 it will be contam free at least for the time while im working , and also wanted to know if the h2o2 harms de mycelium or any of the shroom related stuff
For the sanitization of surfaces and working areas, use straight isopropyl alcohol or a good strong bleach solution.
You can also use lysol, but I fucking hate lysol.
-------------------- "Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury All of my posts are full of fiction and blatant lies.
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OldSpice
Geritol Breath...
Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
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Post deleted by Administrator [Re: Anno]
#1856827 - 08/27/03 09:48 PM (20 years, 7 months ago) |
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-------------------- So hard to be ....WDWGFH? Texas is humongus compared to France Our Gair, who art in Texas, Paw Paw be thy Name.... My friends are thirsty You never see a motorcycle parked outside a Psychiatrist office
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shakta
Infidel
Registered: 06/03/03
Posts: 2,633
Last seen: 19 years, 10 months
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Re: H2O2 Sterilizing [Re: OldSpice]
#1856898 - 08/27/03 10:03 PM (20 years, 7 months ago) |
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How is this a needless question? The guy is having problems with contams and seeking some help. No Anno is not a woman.
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