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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Sclerotia cultivation with Frank - p. tampanensis 2
#18503360 - 07/02/13 05:43 PM (11 years, 6 months ago) |
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Since I had decent success with my galindoi jars I wanted to try tampanensis. A member was kind enough to provide me with a fresh print.
I moved the spores to agar in may and took a couple transfers from healthy looking sections. I inoculated 6 jars of grains on the 23rd (with an agar wedge) and now the healthy growth is beginning to become visible in 2/3 of the jars.
These were all inoculated in "open air" inside my draft-free tent. I'm very pleased with the results so far.
Here are the jars, I'll update this every so often until the harvest in a few months.

Edited by FrankHorrigan (07/02/13 05:50 PM)
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mshaw21
I think i just


Registered: 04/29/11
Posts: 774
Loc: Out of Town
Last seen: 7 years, 6 months
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18503668 - 07/02/13 06:46 PM (11 years, 6 months ago) |
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GOOD LUCK!
-------------------- crossed the line
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: mshaw21]
#18503764 - 07/02/13 07:06 PM (11 years, 6 months ago) |
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Hell yeah - I'll be watching!
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tbagtag
Boomer Barron

Registered: 01/16/13 
Posts: 1,432
Loc: Amsterdam
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: mycomattie]
#18503774 - 07/02/13 07:07 PM (11 years, 6 months ago) |
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Frank - are you going to be doing prints of these? I have been wanting to do stones for kicks and grins.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: tbagtag]
#18503781 - 07/02/13 07:09 PM (11 years, 6 months ago) |
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Quote:
mycomattie said:
Hell yeah - I'll be watching!
You sexy devil, you 
Quote:
tbagtag said: Frank - are you going to be doing prints of these? I have been wanting to do stones for kicks and grins.
Yeah but I'm going to do stones first which will take 4-6 months depending on how long I want to let them sit in the jar.
I have some p. galindoi I'm attempting to fruit right now. Keep an eye out in the marketplace.
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tbagtag
Boomer Barron

Registered: 01/16/13 
Posts: 1,432
Loc: Amsterdam
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18503795 - 07/02/13 07:11 PM (11 years, 6 months ago) |
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ah shit im going to have to make some prints. I got some B+ but those fuckers are giant. I'll have some Thai Lipa Yai coming up so I'll have to stalk you in the MP.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: tbagtag]
#18503810 - 07/02/13 07:15 PM (11 years, 6 months ago) |
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Quote:
tbagtag said: I'll have to stalk you
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tbagtag
Boomer Barron

Registered: 01/16/13 
Posts: 1,432
Loc: Amsterdam
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18503869 - 07/02/13 07:33 PM (11 years, 6 months ago) |
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Timehole



Registered: 04/28/12
Posts: 156
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18505527 - 07/03/13 04:04 AM (11 years, 6 months ago) |
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: Timehole]
#18506511 - 07/03/13 11:05 AM (11 years, 6 months ago) |
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Looks awesome. I'm a big fan of SS's method of using LC for sclerotia producers. A lot of times they won't recover from a shake, so starting with LC all over the grain seems to get it done a lot faster.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: 36fuckin5]
#18506527 - 07/03/13 11:09 AM (11 years, 6 months ago) |
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Quote:
36fuckin5 said: Looks awesome. I'm a big fan of SS's method of using LC for sclerotia producers. A lot of times they won't recover from a shake, so starting with LC all over the grain seems to get it done a lot faster.

I actually have some ELME ready for that very purpose.
I left some mycelium on my plates when I knocked up the jars. There are stones beginning to form so I am going to be transferring those to new plates. After isolating from them I'll inoculate a few 120cc LC jars with those wedges and make a big project out of it.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18506613 - 07/03/13 11:33 AM (11 years, 6 months ago) |
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Quote:
FrankHorrigan said: After isolating from them I'll inoculate a few 120cc LC jars with those wedges and make a big project out of it.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 10 years, 8 months
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: 36fuckin5]
#18506699 - 07/03/13 11:57 AM (11 years, 6 months ago) |
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 Tamp has always been my favorite stone producer. Ive been trying to start it up again. Ive tried 3 different prints all with no success. I always got huge stones with tamp's hope you get some good results.
--------------------
  WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH
Need help? Feel free to me.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: PrinceShroom]
#18506759 - 07/03/13 12:14 PM (11 years, 6 months ago) |
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I'm pretty excited myself.
I'd been trying to get actual tampanensis spores for well over a year. Three different times my "tampanensis" spores were actually cubes 
Thankfully mycomattie saved the day and here we are
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Uhurungus
Avoiderer of Bullshit


Registered: 04/20/13
Posts: 838
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18507144 - 07/03/13 02:15 PM (11 years, 6 months ago) |
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do you see any disadvantages of doing this in pint jars instead of quart?
-------------------- I'm never 100% on anything, just close enough to risk looking foolish.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: Uhurungus]
#18507150 - 07/03/13 02:16 PM (11 years, 6 months ago) |
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Quote:
Uhurungus said: do you see any disadvantages of doing this in pint jars instead of quart?
Smaller yield, but other than that it works fine.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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KevinDontWave
Kiwi Cat



Registered: 08/22/09
Posts: 2,341
Loc: The Desert of the Mind
Last seen: 2 hours, 51 minutes
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: 36fuckin5]
#18507347 - 07/03/13 03:06 PM (11 years, 6 months ago) |
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--------------------
     
Pigs treat us as equals. Never wrestle with pigs.
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Uhurungus
Avoiderer of Bullshit


Registered: 04/20/13
Posts: 838
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: KevinDontWave]
#18507432 - 07/03/13 03:31 PM (11 years, 6 months ago) |
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excellent. soaking grain for 12 pints now! thank you mycomattie!
 
first pic, top dish, i'm thinking that's primordia forming? second gen agar i had fruits in 2 of 4 dishes. thoughts?
-------------------- I'm never 100% on anything, just close enough to risk looking foolish.
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itsaconspiracy
governments worst nightmare



Registered: 03/26/13
Posts: 2,807
Loc: florida
Last seen: 3 years, 4 months
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: Uhurungus]
#18507817 - 07/03/13 05:25 PM (11 years, 6 months ago) |
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I'll be watching
-------------------- ("i'm not a criminal i just like stuff thats not mine")
"bender"
 
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: Uhurungus]
#18513888 - 07/04/13 11:03 PM (11 years, 6 months ago) |
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Quote:
Uhurungus said: excellent. soaking grain for 12 pints now! thank you mycomattie!
 
first pic, top dish, i'm thinking that's primordia forming? second gen agar i had fruits in 2 of 4 dishes. thoughts?
Looks like you've got stones forming on your culture. Transfer those. Those will be badass fruiters nine times out of ten
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