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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Sclerotia cultivation with Frank - p. tampanensis 2
#18503360 - 07/02/13 05:43 PM (11 years, 6 months ago) |
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Since I had decent success with my galindoi jars I wanted to try tampanensis. A member was kind enough to provide me with a fresh print.
I moved the spores to agar in may and took a couple transfers from healthy looking sections. I inoculated 6 jars of grains on the 23rd (with an agar wedge) and now the healthy growth is beginning to become visible in 2/3 of the jars.
These were all inoculated in "open air" inside my draft-free tent. I'm very pleased with the results so far.
Here are the jars, I'll update this every so often until the harvest in a few months.

Edited by FrankHorrigan (07/02/13 05:50 PM)
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mshaw21
I think i just


Registered: 04/29/11
Posts: 774
Loc: Out of Town
Last seen: 7 years, 6 months
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18503668 - 07/02/13 06:46 PM (11 years, 6 months ago) |
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GOOD LUCK!
-------------------- crossed the line
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: mshaw21]
#18503764 - 07/02/13 07:06 PM (11 years, 6 months ago) |
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Hell yeah - I'll be watching!
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tbagtag
Boomer Barron

Registered: 01/16/13 
Posts: 1,432
Loc: Amsterdam
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: mycomattie]
#18503774 - 07/02/13 07:07 PM (11 years, 6 months ago) |
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Frank - are you going to be doing prints of these? I have been wanting to do stones for kicks and grins.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: tbagtag]
#18503781 - 07/02/13 07:09 PM (11 years, 6 months ago) |
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Quote:
mycomattie said:
Hell yeah - I'll be watching!
You sexy devil, you 
Quote:
tbagtag said: Frank - are you going to be doing prints of these? I have been wanting to do stones for kicks and grins.
Yeah but I'm going to do stones first which will take 4-6 months depending on how long I want to let them sit in the jar.
I have some p. galindoi I'm attempting to fruit right now. Keep an eye out in the marketplace.
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tbagtag
Boomer Barron

Registered: 01/16/13 
Posts: 1,432
Loc: Amsterdam
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18503795 - 07/02/13 07:11 PM (11 years, 6 months ago) |
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ah shit im going to have to make some prints. I got some B+ but those fuckers are giant. I'll have some Thai Lipa Yai coming up so I'll have to stalk you in the MP.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: tbagtag]
#18503810 - 07/02/13 07:15 PM (11 years, 6 months ago) |
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Quote:
tbagtag said: I'll have to stalk you
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tbagtag
Boomer Barron

Registered: 01/16/13 
Posts: 1,432
Loc: Amsterdam
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18503869 - 07/02/13 07:33 PM (11 years, 6 months ago) |
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Timehole



Registered: 04/28/12
Posts: 156
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18505527 - 07/03/13 04:04 AM (11 years, 6 months ago) |
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: Timehole]
#18506511 - 07/03/13 11:05 AM (11 years, 6 months ago) |
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Looks awesome. I'm a big fan of SS's method of using LC for sclerotia producers. A lot of times they won't recover from a shake, so starting with LC all over the grain seems to get it done a lot faster.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: 36fuckin5]
#18506527 - 07/03/13 11:09 AM (11 years, 6 months ago) |
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Quote:
36fuckin5 said: Looks awesome. I'm a big fan of SS's method of using LC for sclerotia producers. A lot of times they won't recover from a shake, so starting with LC all over the grain seems to get it done a lot faster.

I actually have some ELME ready for that very purpose.
I left some mycelium on my plates when I knocked up the jars. There are stones beginning to form so I am going to be transferring those to new plates. After isolating from them I'll inoculate a few 120cc LC jars with those wedges and make a big project out of it.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18506613 - 07/03/13 11:33 AM (11 years, 6 months ago) |
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Quote:
FrankHorrigan said: After isolating from them I'll inoculate a few 120cc LC jars with those wedges and make a big project out of it.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 10 years, 8 months
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: 36fuckin5]
#18506699 - 07/03/13 11:57 AM (11 years, 6 months ago) |
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 Tamp has always been my favorite stone producer. Ive been trying to start it up again. Ive tried 3 different prints all with no success. I always got huge stones with tamp's hope you get some good results.
--------------------
  WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH
Need help? Feel free to me.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: PrinceShroom]
#18506759 - 07/03/13 12:14 PM (11 years, 6 months ago) |
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I'm pretty excited myself.
I'd been trying to get actual tampanensis spores for well over a year. Three different times my "tampanensis" spores were actually cubes 
Thankfully mycomattie saved the day and here we are
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Uhurungus
Avoiderer of Bullshit


Registered: 04/20/13
Posts: 838
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18507144 - 07/03/13 02:15 PM (11 years, 6 months ago) |
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do you see any disadvantages of doing this in pint jars instead of quart?
-------------------- I'm never 100% on anything, just close enough to risk looking foolish.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: Uhurungus]
#18507150 - 07/03/13 02:16 PM (11 years, 6 months ago) |
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Quote:
Uhurungus said: do you see any disadvantages of doing this in pint jars instead of quart?
Smaller yield, but other than that it works fine.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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KevinDontWave
Kiwi Cat



Registered: 08/22/09
Posts: 2,341
Loc: The Desert of the Mind
Last seen: 2 hours, 54 minutes
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: 36fuckin5]
#18507347 - 07/03/13 03:06 PM (11 years, 6 months ago) |
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--------------------
     
Pigs treat us as equals. Never wrestle with pigs.
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Uhurungus
Avoiderer of Bullshit


Registered: 04/20/13
Posts: 838
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: KevinDontWave]
#18507432 - 07/03/13 03:31 PM (11 years, 6 months ago) |
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excellent. soaking grain for 12 pints now! thank you mycomattie!
 
first pic, top dish, i'm thinking that's primordia forming? second gen agar i had fruits in 2 of 4 dishes. thoughts?
-------------------- I'm never 100% on anything, just close enough to risk looking foolish.
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itsaconspiracy
governments worst nightmare



Registered: 03/26/13
Posts: 2,807
Loc: florida
Last seen: 3 years, 4 months
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: Uhurungus]
#18507817 - 07/03/13 05:25 PM (11 years, 6 months ago) |
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I'll be watching
-------------------- ("i'm not a criminal i just like stuff thats not mine")
"bender"
 
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: Uhurungus]
#18513888 - 07/04/13 11:03 PM (11 years, 6 months ago) |
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Quote:
Uhurungus said: excellent. soaking grain for 12 pints now! thank you mycomattie!
 
first pic, top dish, i'm thinking that's primordia forming? second gen agar i had fruits in 2 of 4 dishes. thoughts?
Looks like you've got stones forming on your culture. Transfer those. Those will be badass fruiters nine times out of ten
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 2 years, 4 months
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18513990 - 07/04/13 11:42 PM (11 years, 6 months ago) |
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PS
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Uhurungus
Avoiderer of Bullshit


Registered: 04/20/13
Posts: 838
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: PrimalSoup]
#18514202 - 07/05/13 01:21 AM (11 years, 6 months ago) |
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too late. i figured they were the beginnings of actual fruit. last transfer i had 3 actual pins that i put on seperate dishes so i thought i had my bases covered with them. i never even considered they might be stones. i took both dishes in the left pic and innoc'd 14 pints today. we shall see what happens.
the dishes i transfered the pins on are still growing out. 1 looks nice and healthy and the other 2 are lagging and somewhat sickly looking.
-------------------- I'm never 100% on anything, just close enough to risk looking foolish.
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gornyhuy
A Myth Intrepidly Met



Registered: 08/04/09
Posts: 2,933
Loc: USA
Last seen: 3 years, 2 months
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: Uhurungus]
#18763970 - 08/27/13 01:35 PM (11 years, 4 months ago) |
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Any updates? Inquiring minds want to know.
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nhmi
Stranger
Registered: 08/18/13
Posts: 44
Last seen: 11 years, 20 days
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: gornyhuy]
#18764122 - 08/27/13 02:13 PM (11 years, 4 months ago) |
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hope its going good man
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Carion
Blazin
Registered: 08/05/13
Posts: 24
Last seen: 10 years, 4 months
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: nhmi]
#18764319 - 08/27/13 02:55 PM (11 years, 4 months ago) |
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Was thinking of doing something like this. Wll be happy to see the results when they come :-)
-------------------- New but learning!
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: Carion]
#18764382 - 08/27/13 03:08 PM (11 years, 4 months ago) |
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Lemme see yer stones!!!
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: 36fuckin5]
#18764686 - 08/27/13 04:09 PM (11 years, 4 months ago) |
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This round is not going to make it full term.
I was trying out some different lid filters (cellulose filter disks) and they allow far too much GE, more than I anticipated.
As a result the lower 3/4 of the jars are fully colonized and making stones. The upper 1/4 are dried out and some of them getting random green patches on the very top.
No more of these cellulose filter disks for me 
I do have a very beautiful LC (tampanensis isolate) and a whole lot of rye to prep for it.

This particular isolate is one of nine that were acquired by transferring stones that formed on the first petri dish. It was an MS dish initially.
I'm not sure when I will get around to this though.
I will probably just do six to start, and start the cultures on agar from the LC.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18764784 - 08/27/13 04:30 PM (11 years, 4 months ago) |
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Sounds to me like you need to go ahead and spawn what you have before it wants to go bad.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Uhurungus
Avoiderer of Bullshit


Registered: 04/20/13
Posts: 838
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: 36fuckin5]
#18807191 - 09/06/13 12:03 PM (11 years, 4 months ago) |
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these are mine nearly 2 months to the day. i'm getting ready to harvest and spawn to bulk cause i'm a bit sketchy on the colors in some of these jars. first time growing stones and i've seen some pictures but not much confidence on really knowing what's going on here.
i used standard lids with a half inch hole in the middle and 1 layer of post office tyvek between glass and lid. some definite drying out and shrinkage. some definite metabolite production. some moisture still inside.
-------------------- I'm never 100% on anything, just close enough to risk looking foolish.
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budkatz

Registered: 04/12/09
Posts: 87
Last seen: 3 hours, 9 minutes
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18909813 - 09/30/13 02:43 AM (11 years, 3 months ago) |
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Quote:
FrankHorrigan said: This round is not going to make it full term.
I was trying out some different lid filters (cellulose filter disks) and they allow far too much GE, more than I anticipated.
As a result the lower 3/4 of the jars are fully colonized and making stones. The upper 1/4 are dried out and some of them getting random green patches on the very top.
No more of these cellulose filter disks for me 
I do have a very beautiful LC (tampanensis isolate) and a whole lot of rye to prep for it.

This particular isolate is one of nine that were acquired by transferring stones that formed on the first petri dish. It was an MS dish initially.
I'm not sure when I will get around to this though.
I will probably just do six to start, and start the cultures on agar from the LC.
I had the same thing happen as far as the upper portions of the grain drying out using a cellulose filter disks lid tek on stone producers. Maybe the hole in the lid was too big allowing too much GE. Bottom portion of the grain looked great. Is there a better recommendation for how to make a lid for stone producers that will be staying in jars for extended periods?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: budkatz]
#18912273 - 09/30/13 05:41 PM (11 years, 3 months ago) |
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I grew p. galindoi sclerotia with my regular grain lids. 1/8" hole covered by SFD. They did not have any issues
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budkatz

Registered: 04/12/09
Posts: 87
Last seen: 3 hours, 9 minutes
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: FrankHorrigan]
#18913238 - 09/30/13 09:16 PM (11 years, 3 months ago) |
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Cool, I'll just use the regular sfd's. Had access to the cellulose ones, but they didn't work as I had hoped.
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blojo02184
Big Red



Registered: 05/15/13
Posts: 3,527
Loc: Maine
Last seen: 6 months, 21 days
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: budkatz]
#18978847 - 10/14/13 09:34 PM (11 years, 3 months ago) |
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hey budkatz, what material are you thinking of when you say SFD? ptfe? polypropelene? pvdf? pes? nylon?
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botanicalbishop


Registered: 12/22/12
Posts: 93
Loc: the abyss
Last seen: 3 years, 28 days
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: blojo02184]
#18982197 - 10/15/13 04:56 PM (11 years, 3 months ago) |
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He means synthetic filter disc, it can be anything from eazy felt to the actual brand. What they all have in common is they are made out of polyfill
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blojo02184
Big Red



Registered: 05/15/13
Posts: 3,527
Loc: Maine
Last seen: 6 months, 21 days
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: botanicalbishop]
#18987861 - 10/16/13 07:40 PM (11 years, 3 months ago) |
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p.s. sorry for thread jacking frank
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captain trips99
FNG


Registered: 10/08/13
Posts: 28
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Re: Sclerotia cultivation with Frank - p. tampanensis [Re: blojo02184]
#19024537 - 10/24/13 09:07 AM (11 years, 2 months ago) |
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First time grower. Going to try stones on WB and RB. Also going try PF Tek for shrooms at the same time. Any info would be helpful
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