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Offlinenetpumber
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Registered: 06/30/13
Posts: 1
Last seen: 10 years, 8 months
Morphological mycorhizal fungi identification
    #18491538 - 06/30/13 09:01 AM (10 years, 8 months ago)

Hello guyz!

I found this community while i was searching about a recipe for a cultivation medium. So in our subject now. I isolate some fungi from a plant's roots using PDA medium + antibiotics.

Fungi grew up and replate it into a pure PDA medium.
Now i want to try to identify it morphological and i want to ask you how to do this. I have to replate it in a specific medium under specific conditions to make it produce spores? Is there a specific technique i had to follow ?

Does anyone else tried that? 

Thanks in advance!

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InvisibleinskiM
Cortinariologist
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Registered: 02/28/06
Posts: 5,767
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Re: Morphological mycorhizal fungi identification [Re: netpumber]
    #18495457 - 07/01/13 01:06 AM (10 years, 8 months ago)

Hello and welcome,

Your question belongs in the Advanced Mycology Forum but I don't think there will be anyone there who could answer your question any better than the members in this Forum.

It will be very difficult to come to any conclusion with your experiment and the only two methods I can think of are ...

If you know what the specific tree host was you could grow some of that tree under tissue culture and inoculate the root system with a culture you have isolated, it needs to be a pure culture with no contamination present, grow the trees on in an ideal environment and it is possible that you could get some fruit bodies forming, this method requires some specialised equipment and a lot of patience and time...

…Or, once you have a pure isolate send the resulting mycelium away to be DNA sequenced, then compare that sequence with data you can find at BLAST.

Good luck, I'm interested to hear about your results if you can complete the experiments:cool:


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Offlineobtuse
myco0
I'm a teapot


Registered: 02/18/09
Posts: 2,406
Loc: tasmania Flag
Last seen: 1 year, 7 months
Trusted Identifier
Re: Morphological mycorhizal fungi identification [Re: inski]
    #18495482 - 07/01/13 01:19 AM (10 years, 8 months ago)

I would attempt to clean the culture, and make sure you have a pure culture.

Given the ease with which you can get genetic informtaion these days i would be more inclinded to identify it from DNA.  But then you have one of two paths, depending on how clean your culture, to extract DNA from your isolate, PCR it to clone and amplify the DNA, and send it away to get it sequenced, and hopefully find a match in BLAST once you have your results.

alternatively, you may in fact have captured a community, in which case you may have to do a community fingerprint analysis, and one of the possible techniques to do that.  http://en.wikipedia.org/wiki/Community_Fingerprinting

Inski mentioned tissue culture, Depending on your skill set, that may also be a good alternative.

Good luck :laugh:

Edited by obtuse (07/01/13 01:20 AM)

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