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OfflineJcoonan
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Registered: 06/14/12
Posts: 170
Last seen: 10 years, 9 months
Inhibit Phosphotase enzyme during Psilocybin extraction
    #18488870 - 06/29/13 04:24 PM (10 years, 10 months ago)

Not sure if this belongs here but here goes.

One of the major problems extracting Psilocybin seems to be phosphorylation of Psilocybin, addition of a phosphorous group, which changes it into Psilocin, the biologically active but extremely unstable version of the spice. Has anyone taken steps or heard of steps being taken to disable the phosphotase enzyme responsible, there by dramatically increasing the Psilocybin in the extract which would improve shelf life?


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InvisibleStygianKnight
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Registered: 03/12/12
Posts: 2,717
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Re: Inhibit Phosphotase enzyme during Psilocybin extraction [Re: Jcoonan]
    #18517563 - 07/05/13 07:00 PM (10 years, 10 months ago)

Unfortunately you have it backwards, Psilocybin is stable and contains phosphate, psilocin is less stable and does not contain phosphate.

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Offlinecubenpete
Aminita good excuse
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Registered: 03/13/12
Posts: 837
Loc: Kentucky
Last seen: 2 years, 7 months
Re: Inhibit Phosphotase enzyme during Psilocybin extraction [Re: StygianKnight]
    #18523378 - 07/07/13 01:54 AM (10 years, 10 months ago)

:popcorn:

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OfflineCrusaderz
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Registered: 07/27/12
Posts: 145
Last seen: 9 years, 4 months
Re: Inhibit Phosphotase enzyme during Psilocybin extraction [Re: cubenpete]
    #18523436 - 07/07/13 02:22 AM (10 years, 10 months ago)

add something for the enzyme to bind to and never let go of

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Offlinematsc
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Registered: 09/17/12
Posts: 681
Loc: Arizona
Last seen: 6 months, 26 days
Re: Inhibit Phosphotase enzyme during Psilocybin extraction [Re: Crusaderz]
    #18523475 - 07/07/13 02:42 AM (10 years, 10 months ago)

Off the top of my head I do not know many non-competitive or irreversible  phosphatase inhibitors. At least, none that are even remotely safe to use. Competitive inhibition is pretty easy though, just toss in phosphate or pyrophosphate compounds. Hell Alkaline phosphatase inhibition is one of the most commonly performed biochemical experiments on the planet. Anyone who has ever taken a basic biochem lab has sat through it.

But thats not really the issue. Oxidation is much more of a worry if you are working with psilocybin. Once in the body psilocybin is dephosphorylated to psilocin anyway.

EDIT:
Oh and for the record, if an enzyme ADDS a phosphate its a kinase, if it REMOVES a phosphate its a phosphatase.


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Edited by matsc (07/07/13 02:49 AM)

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