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Offlineshroomress
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fresh air in incubation chamber to avoid bacillus?
    #18451095 - 06/21/13 09:26 AM (11 years, 6 months ago)

Hi, I'm still new to this and would appreciate some advice.
I have just inoc. my 3rd grow.
Have had issues with sour smell/bacillus but most of my jars recovered, just took longer to colonize.
Probably my moisture content not quite right and also put some trays of HPoo in chamber that really soured up because I didn't put vent holes into foil.
I have been leaving the Incubation chamber half open and seems to help get rid of bacillus.
My question is - should there be some air circulation in incubation chamber? will that reduce sour/bacterial contam?
Thanks

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InvisibleFrankHorrigan
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Re: fresh air in incubation chamber to avoid bacillus? [Re: shroomress]
    #18451224 - 06/21/13 10:04 AM (11 years, 6 months ago)

No, if your grains have bacteria they need to be tossed, not spawned.

There is no "winning" against live bacteria or molds on grains :frown:

Proper sterile technique while making and inoculating your spawn in very important, as is proper pasteurization.  Use a still air box for all grain work.


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Offlineganjfather
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Re: fresh air in incubation chamber to avoid bacillus? [Re: shroomress]
    #18451282 - 06/21/13 10:19 AM (11 years, 6 months ago)

Why are you using an incubation chamber at all?


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Offlineshroomress
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Re: fresh air in incubation chamber to avoid bacillus? [Re: shroomress]
    #18451313 - 06/21/13 10:26 AM (11 years, 6 months ago)

thanks for your response.

Without wanting to seem like I know it all, being a noob at this, I do have experience with sterility (operating rooms). I would contend that you cannot avoid all bacteria no matter what method of sterility you use. Being more sterile just reduces the numbers greatly.
If you have wet cakes the few bacteria that got in (unavoidable) will take hold, were as dry cakes are a less good medium for them.

I have had sour cakes that recovered after taking micropore off holes and leaving lids very loose. The mycelium took over and sour smell disappeared.

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Offlineshroomress
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Re: fresh air in incubation chamber to avoid bacillus? [Re: shroomress]
    #18451328 - 06/21/13 10:29 AM (11 years, 6 months ago)

My house goes into mid 60s at night, but maybe I should just incubate at room temp in closet.
I did have wet cakes contaminate but grain jars in same chamber stay good. The grain was undercooked, nearly raw looking and very dry.

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Offlineganjfather
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Re: fresh air in incubation chamber to avoid bacillus? [Re: shroomress]
    #18451337 - 06/21/13 10:32 AM (11 years, 6 months ago)

Quote:

shroomress said:
My house goes into mid 60s at night, but maybe I should just incubate at room temp in closet.
I did have wet cakes contaminate but grain jars in same chamber stay good. The grain was undercooked, nearly raw looking and very dry.





Mid 60s is fine, man. Keep them at room temp, incubation chambers are way outdated and contam prone. My apartment fluctuates from 65 at night to 80 during the hottest part of the day. A fluctuating temp is more like nature anyway.

Keep them at room temp, no need to make an incubation chamber so half your projects get contamed when room temp is optimal.


THROW YOUR INCUBATION CHAMBER IN THE GARBAGE!


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Offlineshroomress
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Re: fresh air in incubation chamber to avoid bacillus? [Re: shroomress]
    #18451379 - 06/21/13 10:47 AM (11 years, 6 months ago)

Thanks, I think I may just retire my incubation chamber.

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Invisible36fuckin5
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Re: fresh air in incubation chamber to avoid bacillus? [Re: shroomress]
    #18451399 - 06/21/13 10:54 AM (11 years, 6 months ago)

The thing is that none of this is 100% sterile. Apparently you know about sterile procedure and how it's not quite exact, just good enough. Especially since the harshest thing we use (besides flame) is isopropyl alcohol.

It's all a race against contamination. Bacteria is considered a contamination, especially in your grain jars. What we're doing is just giving our mycelium a head start over all the other molds and bacteria. If you allow another organism to proliferate, it's going to eat all the food and drink all the water that you put in for your mushrooms.

So that's why you keep failing. Never spawn bacterial jars. They won't "beat" the bacteria, they'll just get overtaken.


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Offlineshroomress
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Re: fresh air in incubation chamber to avoid bacillus? [Re: shroomress]
    #18451508 - 06/21/13 11:27 AM (11 years, 6 months ago)

why is it that BRF cakes can recover from bacteria but grains can't?

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