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ganjfather
uncle randy



Registered: 08/06/09
Posts: 6,342
Loc:
Last seen: 4 years, 6 months
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Re: confused about the whole a cube is a cube is a cube is a... [Re: FrankHorrigan]
#18449733 - 06/20/13 10:25 PM (11 years, 6 months ago) |
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Quote:
FrankHorrigan said:
Quote:
ganjfather said: You can cotton swab a PE, but the cotton is much more prone to contaminants than foil is, so making a spore syringe from a swab is a bad idea because 90% of the time it contams.
You need to sterilize your swabs in a jar in your PC. Then after taking the spore swab, you need to let the swab dry in a still-air box for 48 hours or so. This will reduce contams significantly.
I honestly go PE from time to time but I love the variation of all different genetics. Plus I can get better yields more consistently with less work using other varieties 
Good to know!
All the swabs I've gotten have been from other people, so I don't trust their technique, I'd still go straight to agar with the swabs.
I've found oak ridge to be more potent than most other cubes, as well. A good oak ridge isolate is great
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
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Re: confused about the whole a cube is a cube is a cube is a... [Re: Growth]
#18450314 - 06/21/13 01:47 AM (11 years, 6 months ago) |
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Quote:
Growth said: sounds complicated...
You're in luck. Stropharis just made an excellent pictorial guide on agar isolation. Check it out:
http://www.shroomery.org/forums/showflat.php/Number/18430998
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Solipsis
m̶a̶d̶ disappointed scientist



Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands
Last seen: 1 year, 5 months
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Re: confused about the whole a cube is a cube is a cube is a... [Re: Psilicon]
#18450835 - 06/21/13 07:34 AM (11 years, 6 months ago) |
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So there is genetic variation and calling something a mutant basically means that in a special case the genes cause the phenotype to be different in a noticeable / measurable way.
That can be about appearance (like albinism or leucism or malformations) but also about metabolism.
It just may be that not every type of mutation happens in the same way. If one fruit lacks genes to express pigment you get albino's or leucistic ones... but the potency results from presence of alkaloids that are part of a metabolism that is not expressed by just one gene. So to change something so rigorously it may take more generations of selection. It may take MANY quantitative analysis tests to make sure you continue with the most potent offspring every time. And I guess if you keep pushing the envelope you can make weird strains, but the unusual tendencies that PE may have like producing malformed fruits the first flush, looking generally gnarly and getting less consistent grows / yields are probably signs that mutation upon mutation has added up over all those generations in order to increase potency. A sort of tradeoff or drawback.
At least those are my ideas about it, do you guys think I'm on the right track?
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HypnotoadCroaked
Retired, but will check MSGs


Registered: 01/05/13
Posts: 1,168
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Re: confused about the whole a cube is a cube is a cube is a... [Re: cronicr]
#18450853 - 06/21/13 07:45 AM (11 years, 6 months ago) |
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Quote:
cronicr said:
pe is not known for heavy spore deposits
The typical PE "Style" is not known for heavy spore deposits at all. However from seeing a few grows, I had wondered if the normal style caps sometimes would deposit spores more readily, AND produce spores that would continue the mutation? There are from time to time PE growths that look like a normal cube. Just something that was on my mind.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: confused about the whole a cube is a cube is a cube is a... [Re: HypnotoadCroaked]
#18450857 - 06/21/13 07:47 AM (11 years, 6 months ago) |
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Quote:
Whippy said: The typical PE "Style" is not known for heavy spore deposits at all. However from seeing a few grows, I had wondered if the normal style caps sometimes would deposit spores more readily, AND produce spores that would continue the mutation? There are from time to time PE growths that look like a normal cube. Just something that was on my mind.
IME spores from "normal" PE caps are still rare.
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