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OfflineJackthetripper7
Male


Registered: 05/14/12
Posts: 369
Loc: Down the rabbit hole
Last seen: 10 years, 21 days
Proper Gas Exchange
    #18442887 - 06/19/13 03:11 PM (11 years, 6 months ago)

What's up shroomerites? I've just nocced' up 5 pint jars with P. Costa Rica last night, and they're sitting snugly on my bookshelf behind a row of books. I wiped everything surface/dusty looking object in my room down with Lysol, washed my bed sheets, vacuumed, and vigorously sprayed the room with oust (For those of you who like to read as much as I do, I'm sorry but the books had to get it :smirk:). I know it may sound OD, but since I'm using pint jars I'm trying to take every precaution in my power to ensure a sterile environment for them to colonize in. My question about gas exchange is, is it okay to open my windows and let in fresh air? I know they need gas exchange to thrive, but I feel like I'd be introducing airborne contams into my OD sterile environment. I know the dry verm barrier is a filter for contams, but I guess I'm just paranoid :eek:. I've read through the forums, but none have addressed this specifically. Let me know what you guys think I should do.


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:goodluckwiththat:

Edited by Jackthetripper7 (06/19/13 03:16 PM)

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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: Proper Gas Exchange [Re: Jackthetripper7]
    #18442909 - 06/19/13 03:16 PM (11 years, 6 months ago)

The cakes have a dry verm layer, which is the contam filter.

They will be fine in a dusty ass room on a shelf, sitting next to a pile of shit, as long as the dry verm layer is intact.

Open the windows if you want, it will not affect anything.

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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
Re: Proper Gas Exchange [Re: PussyFart]
    #18442965 - 06/19/13 03:28 PM (11 years, 6 months ago)

Quote:

Notahacker420 said:
The cakes have a dry verm layer, which is the contam filter.

They will be fine in a dusty ass room on a shelf, sitting next to a pile of shit, as long as the dry verm layer is intact.

Open the windows if you want, it will not affect anything.




:whathesaid:

If the tek you're using relies on your bedroom being sterile, you've already lost.  If you're doing PF cakes, the verm is the filter.  If you're doing grains the polyfill is the filter.  Both of these are very effective, and as long as you don't hose them down with shitwater or shake a PF cake, any contamination will likely be from the inside due to improper sterilization.


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Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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InvisibleStygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
Trusted Cultivator
Re: Proper Gas Exchange [Re: Jackthetripper7]
    #18443186 - 06/19/13 04:06 PM (11 years, 6 months ago)

Quote:

Jackthetripper7 said:
ensure a sterile environment for them to colonize in.



Your room is cleaner, but it's not sterile.  Opening a window is fine.
As long as the barriers don't get wet, they should work fine.

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OfflineJackthetripper7
Male


Registered: 05/14/12
Posts: 369
Loc: Down the rabbit hole
Last seen: 10 years, 21 days
Re: Proper Gas Exchange [Re: StygianKnight]
    #18443303 - 06/19/13 04:29 PM (11 years, 6 months ago)

I'm using pf tek, I know I'm just very paranoid about contams (it's been a while since I've done a project!) I just want to do everything and anything I can to make my chances of success go up. using probability, there's a 1/3 rate of success. 1st probability is that they fully colonize, 2nd is that they contam, 3rd is that they stall (thank you algebra 1!) but yeah I'm just gonna open my windows and just leave them be, besides my weekly check on em'.


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:goodluckwiththat:

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Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 2 years, 2 months
Re: Proper Gas Exchange [Re: Jackthetripper7]
    #18443388 - 06/19/13 04:51 PM (11 years, 6 months ago)

You got probability wrong :smile: it's more like 0.9x0.9x0.9=0.72 = 72% considering you'd have 90% success rate at each of those points.


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How I do glass dishes

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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: Proper Gas Exchange [Re: poisoned]
    #18443416 - 06/19/13 04:56 PM (11 years, 6 months ago)

Quote:

poisoned said:
You got probability wrong :smile: it's more like 0.9x0.9x0.9=0.72 = 72% considering you'd have 90% success rate at each of those points.



:mygoditsfullofstars:

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