Home | Community | Message Board

World Seed Supply
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate   MagicBag.co Certified Organic All-In-One Grow Bags   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineHiltster2
Mr. Kite
 User Gallery


Registered: 06/11/13
Posts: 96
Loc: Sgt. Peppers Lonely Heart...
Last seen: 5 years, 3 months
Please help!!
    #18443008 - 06/19/13 03:36 PM (11 years, 6 months ago)

Here's the deal. A month ago I bought 20 cc of TKS. Got my cakes up and running (Steam sterilizing them and Inoculating on my stove). Well 2 weeks later I had nothing. So I figured I made a mistake and started over. Did the same thing. 2 weeks was yesterday and still nothing. NO COLONIZATION. I bought some amazonian and switched vendors. Did 5 more jars yesterday with those. But I'm worried they wont work either. What is going on? I'm following the instructions perfectly.

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: Please help!! [Re: Hiltster2]
    #18443027 - 06/19/13 03:39 PM (11 years, 6 months ago)

Spores can take up to 3 weeks to germinate and show growth.

Did you use one of our sponsors?

Just a yes or no, please do not name the vendor.

Extras: Filter Print Post Top
Invisiblemycomattie
 User Gallery


Registered: 11/15/12
Posts: 1,323
Re: Please help!! [Re: Hiltster2]
    #18443035 - 06/19/13 03:40 PM (11 years, 6 months ago)

Did you let the jars cool down enough?


--------------------

Extras: Filter Print Post Top
OfflineHiltster2
Mr. Kite
 User Gallery


Registered: 06/11/13
Posts: 96
Loc: Sgt. Peppers Lonely Heart...
Last seen: 5 years, 3 months
Re: Please help!! [Re: mycomattie]
    #18443045 - 06/19/13 03:41 PM (11 years, 6 months ago)

The first try I didn't the second try I cooled overnight and continued in the morning. Sill nothing.

Extras: Filter Print Post Top
OfflineHiltster2
Mr. Kite
 User Gallery


Registered: 06/11/13
Posts: 96
Loc: Sgt. Peppers Lonely Heart...
Last seen: 5 years, 3 months
Re: Please help!! [Re: Hiltster2]
    #18443050 - 06/19/13 03:42 PM (11 years, 6 months ago)

The vendor was a sponsor.

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: Please help!! [Re: Hiltster2]
    #18443058 - 06/19/13 03:43 PM (11 years, 6 months ago)

I would give it another week or so.

Did you shake the syringe before inoculating each jar?

How much solution was used per hole/cake?

Extras: Filter Print Post Top
OfflineHiltster2
Mr. Kite
 User Gallery


Registered: 06/11/13
Posts: 96
Loc: Sgt. Peppers Lonely Heart...
Last seen: 5 years, 3 months
Re: Please help!! [Re: PussyFart]
    #18443075 - 06/19/13 03:46 PM (11 years, 6 months ago)

1cc per jar. I did shake. Also I used the last of the syringe so regardless spores went into at least one cake.

Extras: Filter Print Post Top
Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
Re: Please help!! [Re: Hiltster2]
    #18443078 - 06/19/13 03:46 PM (11 years, 6 months ago)

What is the highest temp of the room they are in during the day?

Extras: Filter Print Post Top
Invisiblegizmo1
Other User Gallery


Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM Flag
Re: Please help!! [Re: mycomattie]
    #18443084 - 06/19/13 03:48 PM (11 years, 6 months ago)

To make sure the jars are cool in the center its best to just let them cool overnight.
Make sure you shake the syringe well before inoculation. If you are doing both of these and still no growth you may have received dead spores..


--------------------
Trade List
🖕🖕🖕
6 hole Mini Monos

Extras: Filter Print Post Top
OfflineHiltster2
Mr. Kite
 User Gallery


Registered: 06/11/13
Posts: 96
Loc: Sgt. Peppers Lonely Heart...
Last seen: 5 years, 3 months
Re: Please help!! [Re: invitro]
    #18443085 - 06/19/13 03:48 PM (11 years, 6 months ago)

75 constantly. I also have a chamber I can get 80 degrees constant but it is hard to promote gas exchange because I have to keep a Lid on it for that temperature.

Extras: Filter Print Post Top
Invisiblegizmo1
Other User Gallery


Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM Flag
Re: Please help!! [Re: invitro]
    #18443114 - 06/19/13 03:54 PM (11 years, 6 months ago)

Quote:

invitro said:
What is the highest temp of the room they are in during the day?



Temp shouldn't be a big problem if he is not seeing mold or any growth unless the temps got extremly high. If the temps were low then growth would be slower but you should still see growth and if thats the case its just a waiting games.
OP maybe try sacrificing a jar by opening it and seeing how it smells.


--------------------
Trade List
🖕🖕🖕
6 hole Mini Monos

Extras: Filter Print Post Top
Invisiblegizmo1
Other User Gallery


Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM Flag
Re: Please help!! [Re: gizmo1]
    #18443124 - 06/19/13 03:55 PM (11 years, 6 months ago)

Id say give them another week or 2 tops if you don't see growth within 2 weeks then count your loses and start a new project with the new syringe.


--------------------
Trade List
🖕🖕🖕
6 hole Mini Monos

Extras: Filter Print Post Top
Invisiblewildernessjunkie
Reshitivest
I'm a teapot


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: Please help!! [Re: gizmo1]
    #18443131 - 06/19/13 03:57 PM (11 years, 6 months ago)

Was that 20cc a single syringe? Or two separate ones?

Extras: Filter Print Post Top
OfflineHiltster2
Mr. Kite
 User Gallery


Registered: 06/11/13
Posts: 96
Loc: Sgt. Peppers Lonely Heart...
Last seen: 5 years, 3 months
Re: Please help!! [Re: gizmo1]
    #18443144 - 06/19/13 03:59 PM (11 years, 6 months ago)

What smell should I look for? and two diff ones. Both of TKS. I'm thinking they were dead. But I just switched vendors.

Extras: Filter Print Post Top
Invisiblewildernessjunkie
Reshitivest
I'm a teapot


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: Please help!! [Re: Hiltster2]
    #18443183 - 06/19/13 04:06 PM (11 years, 6 months ago)

I've had spores take up to 3 weeks to germinate. Give it a bit longer.

Usually a bad syringe contaminates, rather than just does nothing. I would give it some time and then contact the vendor.

Extras: Filter Print Post Top
OfflineHiltster2
Mr. Kite
 User Gallery


Registered: 06/11/13
Posts: 96
Loc: Sgt. Peppers Lonely Heart...
Last seen: 5 years, 3 months
Re: Please help!! [Re: wildernessjunkie]
    #18443200 - 06/19/13 04:09 PM (11 years, 6 months ago)

Do you guys use light when colonizing?

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: Please help!! [Re: Hiltster2]
    #18443323 - 06/19/13 04:33 PM (11 years, 6 months ago)

Yes, jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

Extras: Filter Print Post Top
Offlinethundahoof
S+laughter
Male User Gallery

Registered: 02/03/13
Posts: 951
Last seen: 11 years, 3 months
Re: Please help!! [Re: PussyFart]
    #18443365 - 06/19/13 04:44 PM (11 years, 6 months ago)

I think it is more of a problem in summertime that spores will get too hot during shipping.  I think i fried some spores just being in my safe in a very hot upstairs room.


--------------------

Extras: Filter Print Post Top
OfflineHiltster2
Mr. Kite
 User Gallery


Registered: 06/11/13
Posts: 96
Loc: Sgt. Peppers Lonely Heart...
Last seen: 5 years, 3 months
Re: Please help!! [Re: thundahoof]
    #18443557 - 06/19/13 05:25 PM (11 years, 6 months ago)

So if I left a cabinet open so I could see into it but the light isn't directly lighting the jars?

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: Please help!! [Re: Hiltster2]
    #18443565 - 06/19/13 05:26 PM (11 years, 6 months ago)

Yup...exactly.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate   MagicBag.co Certified Organic All-In-One Grow Bags   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules


Similar ThreadsPosterViewsRepliesLast post
* MAJOR BREAKTHROUGH - FASTER PINNING VIA TEMPERATURE CYCLING
( 1 2 all )
Hannibal88 18,917 25 07/08/07 09:29 AM
by RogerRabbit
* steam temperature / pressure relation ohmatic 2,032 18 01/13/05 11:12 AM
by tripndicular
* Temperature Help TheGreatButtPaca 1,301 13 10/30/04 03:00 PM
by matticus
* controlling temperature? psyke 1,910 6 09/11/01 11:28 PM
by oscill8
* Northern room temperature Dionysus 663 5 06/17/03 11:33 AM
by neutralizer
* Low Temperature Fruiting KnobleJunga 4,883 3 02/14/04 07:58 PM
by Ben_Stein
* 65-71` F = Shroom killing temperatures? MycoJunkie 9,473 9 12/13/04 11:15 PM
by LaughingJim
* potency and temperature yogithehoneybear 5,672 15 04/02/03 06:33 PM
by Smiley_Riley

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,747 topic views. 30 members, 130 guests and 24 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.008 seconds on 14 queries.