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KingCloyster
Forest Mushroom 🍄



Registered: 05/27/13
Posts: 597
Loc: Quetico, Canada
Last seen: 1 year, 4 months
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Cooking MEA agar bottle and glass petri dishes together.
#18442384 - 06/19/13 01:19 PM (10 years, 10 months ago) |
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I plan on pouring 5 plates of MEA in the near future so I can isolate a couple master cultures for LC, master slants, w/e... I want to knock two birds out with one stone to reduce risk of contam, can I just wrap my glass dishes in foil and then put them in the sterilizer with my filtered agar tequila bottle?
What would be the time necessary to cook the dishes and agar together? I dont want to overcook the agar and I dont want to undercook the dishes.
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Jeff
Addict



Registered: 10/06/12
Posts: 1,488
Last seen: 6 years, 5 months
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Re: Cooking MEA agar bottle and glass petri dishes together. [Re: KingCloyster]
#18443972 - 06/19/13 07:07 PM (10 years, 10 months ago) |
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I use 45 minutes for my agar. Empty glass petris will sterilize in that amount of time. They will be fine together.
-------------------- Myco-tek
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,091
Loc: Diving into Mystical Territori...
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Re: Cooking MEA agar bottle and glass petri dishes together. [Re: Jeff]
#18448349 - 06/20/13 05:35 PM (10 years, 10 months ago) |
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I'd do 30 minutes, but it'll be fine. Make sure the plates don't get wet.
-------------------- Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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fortheloveofnature


Registered: 01/28/12
Posts: 1,321
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Re: Cooking MEA agar bottle and glass petri dishes together. [Re: Jeff]
#18449753 - 06/20/13 10:30 PM (10 years, 10 months ago) |
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Quote:
Jeff said: I use 45 minutes for my agar. Empty glass petris will sterilize in that amount of time. They will be fine together.
yea but it usually gets messy when the agar is already in the petri . i prefer doing them separately. just my opinion tho
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TRADE LIST sclerotia info FSPThreads 1-2-3-4 Looking for a confirmed Gymnopilus Luteus print Gymnopilus sp
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PanzerCubed




Registered: 11/22/12
Posts: 2,285
Loc: Nauru
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Re: Cooking MEA agar bottle and glass petri dishes together. [Re: fortheloveofnature]
#18450397 - 06/21/13 02:26 AM (10 years, 10 months ago) |
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He is putting the agar in a bottle and wrapping the petri dish's individually, not with the agar pre poured into them
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fortheloveofnature


Registered: 01/28/12
Posts: 1,321
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Re: Cooking MEA agar bottle and glass petri dishes together. [Re: PanzerCubed]
#18450800 - 06/21/13 06:58 AM (10 years, 10 months ago) |
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oops i need to learn how to read . i am illiterate
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TRADE LIST sclerotia info FSPThreads 1-2-3-4 Looking for a confirmed Gymnopilus Luteus print Gymnopilus sp
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