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Offlinehater666
newbie
Registered: 07/18/03
Posts: 48
Last seen: 18 years, 4 months
mycobags no colonization after 7 days
    #1843745 - 08/24/03 01:06 AM (19 years, 5 months ago)

hello there my friend innoculated 10 mycobags with 2 spore syiringes from sporebank.com (ordered from sporebank.com but the syringes were sent by sporeworks.com weird shit) and after 7 days theres no signs of colonization... he mixed up the substrate thoroughly after innoculating... i've heard bad things about spore syringes from this place, so i'm not expectin to see any colonization... is it cool to re-innoculate now with new spore syringes (different strains) or should he wait a week to see if they colonize... btw the bags are being kept inside a box with 2 heating pads on the lowest setting, with around 4 layers of cloth in between, so the tmeperature should be fine for colonization
thx


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OfflineRavinoff
LethargicSnorting Duck

Registered: 07/15/03
Posts: 686
Last seen: 19 years, 4 months
Re: mycobags no colonization after 7 days [Re: hater666]
    #1843748 - 08/24/03 01:07 AM (19 years, 5 months ago)

wait another week or so, if no growth, then reinoculate


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Invisibleph_plus
Malkawian

Registered: 05/27/03
Posts: 556
Loc: Constantinople
Re: mycobags no colonization after 7 days [Re: Ravinoff]
    #1843791 - 08/24/03 01:34 AM (19 years, 5 months ago)

Can you provide more details about the bags? (pre-sterilized, self-healing injection site, etc.) I don't think there would be a problem about sporeworks' syringes.... (maybe you didn't shake the syringes well before inoculation...?...) Also put a temperature near the bags and see how much the temp. is..... But anyway, wait as Ravinoff said!....


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The word truth...... doesn't make any sense..... As if the word sense...... which isn't the truth.........


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Offlinehater666
newbie
Registered: 07/18/03
Posts: 48
Last seen: 18 years, 4 months
Re: mycobags no colonization after 7 days [Re: ph_plus]
    #1844439 - 08/24/03 07:17 AM (19 years, 5 months ago)

yea they're pre-sterilized self healing grain bags and i shook the syringes real well


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Invisibleblink
eye of horus
 User Gallery

Folding@home Statistics
Registered: 04/01/02
Posts: 11,349
Loc: Geographic Location (Stat...
Re: mycobags no colonization after 7 days [Re: hater666]
    #1844450 - 08/24/03 07:20 AM (19 years, 5 months ago)

Mine took almost a month to start, I blame myself for pesting the bags inspecting for growth... just leave them somewhere dark and warm... then come back to them in about 2-3 weeks... it will be a wonderfilled surprise


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Invisibleph_plus
Malkawian

Registered: 05/27/03
Posts: 556
Loc: Constantinople
Re: mycobags no colonization after 7 days [Re: blink]
    #1845001 - 08/24/03 03:22 PM (19 years, 5 months ago)

Quote:

blinkidiot said:
Mine took almost a month to start, I blame myself for pesting the bags inspecting for growth... just leave them somewhere dark and warm... then come back to them in about 2-3 weeks... it will be a wonderfilled surprise 




obsolutely agreed!...... :grin:


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The word truth...... doesn't make any sense..... As if the word sense...... which isn't the truth.........


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InvisibleSuperbowl Bob
member
Registered: 08/09/03
Posts: 166
Re: mycobags no colonization after 7 days [Re: ph_plus]
    #1845162 - 08/24/03 07:29 PM (19 years, 5 months ago)

I thought I did something wrong, but after 7 days I had some white growth. My closet is hot and humid as fuck, so that helps.

If my bag is slightly colonized, should I shake it up?



my fingers magically typed those words above. wow!


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Offlinemushroom_man
Stranger

Registered: 11/08/02
Posts: 48
Last seen: 19 years, 3 months
Re: mycobags no colonization after 7 days [Re: Superbowl Bob]
    #1845190 - 08/24/03 07:54 PM (19 years, 5 months ago)

Mine took about 2 weeks to really start to show. It now has it's little pins starting.

Laviathanx- yeah shake it up a bit and crumble it around, it will speed up colonization


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I love shrooms!!!!!


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Offlinegarbage

Registered: 06/17/03
Posts: 316
Last seen: 9 years, 3 months
Re: mycobags no colonization after 7 days [Re: mushroom_man]
    #1845715 - 08/25/03 12:20 AM (19 years, 5 months ago)

just make sure you innoculated right around the innoculation site, not in the center of the bag like you may think.


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  Vaporbrothers :smile:


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Offlinehater666
newbie
Registered: 07/18/03
Posts: 48
Last seen: 18 years, 4 months
Re: mycobags no colonization after 7 days [Re: garbage]
    #1845720 - 08/25/03 12:23 AM (19 years, 5 months ago)

well inocculation was near injection sites but then the substrate was all mixued up and shit, so the spores gotta be all over the place, i dunno if that may be a problem


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InvisibleSuperbowl Bob
member
Registered: 08/09/03
Posts: 166
Re: mycobags no colonization after 7 days [Re: hater666]
    #1846217 - 08/25/03 03:41 AM (19 years, 5 months ago)

Squishing the mycellium isn't a problem is it? I found that where there was growth, it was a soft, yet tough chunk of grain substrate. So I just squished it between my fingers through the bag and mixed that up with the rest of the stuff.

Also, when it's fully colonized, should I just leave it in the bag to grow, or do something else? This is my first time.

^^^
above is a false statement


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