Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinesammich
Stranger

Registered: 05/30/13
Posts: 25
Last seen: 11 years, 6 months
Syringe or Grain to Grain
    #18436825 - 06/18/13 12:53 PM (11 years, 6 months ago)

I've just spoken with a trusted cultivator and they told me to start doing grain to grain transfers once I've inoculated successfully on my new bulk method.

Rather than posting it in private and after not really seeing anything too promising from my search efforts, I'd like to place my question here so that others may benefit from it if possible: are there any benefits from grain to grain transfers instead of using prints to make spore syringes? Money is negligible.

Extras: Filter Print Post Top
OnlineSulfurshelfsean
Defender of Cubes
 User Gallery

Registered: 07/29/10
Posts: 4,836
Last seen: 3 minutes, 38 seconds
Re: Syringe or Grain to Grain [Re: sammich]
    #18436882 - 06/18/13 01:04 PM (11 years, 6 months ago)

Do you mean a Grain Liquid Culture as opposed to making spore syringes? Because for one an advantage of doing a g2g transfer is that you've already cultured mycellium FROM spores. If you were to inject sterile water into a jar that was 100% colonized and then suck it back out, you'd have a grain LC, and I hear those are great, but have not tried this method myself yet.


--------------------


Everything is better when it is done ON TOP OF A MOUNTAIN!

Edited by Sulfurshelfsean (06/18/13 01:05 PM)

Extras: Filter Print Post Top
OfflineLiquidSubstrate
Wanderer
Male


Registered: 06/18/13
Posts: 131
Last seen: 8 years, 7 months
Re: Syringe or Grain to Grain [Re: sammich]
    #18436999 - 06/18/13 01:27 PM (11 years, 6 months ago)

I believe the fact that you already have mycelium growth started with G2G transfers makes the process more efficient. Syringe inoculation you will have to wait a bit for the growth to start.

Extras: Filter Print Post Top
InvisibleTranscendingLife
I Don't Need a Life to Live
Male User Gallery


Registered: 06/09/10
Posts: 21,627
Trusted Cultivator
Re: Syringe or Grain to Grain [Re: Sulfurshelfsean]
    #18437019 - 06/18/13 01:31 PM (11 years, 6 months ago)

Grain to Grains are used to expand your clean spawn & cultures.
If you're doing MS inoculation, I'd inoculate 6 jars with 1cc of MS solution.
Take the quickest colonizing one & G2G that to 10 more.

GLCs aren't as shitty as LCs, but they still suck.
They take more time, have more room for error (contams to come in), and have extra steps vs. Grain to Grains.

I can't remember the last time I touched a syringe.
MS-->agar-->isolate-->master grain jar-->G2G-->G2G-->G2G-->spawn.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Syringe or Grain to Grain [Re: TranscendingLife]
    #18437032 - 06/18/13 01:33 PM (11 years, 6 months ago)

Quote:

TranscendingLife said:
Grain to Grains are used to expand your clean spawn & cultures.
If you're doing MS inoculation, I'd inoculate 6 jars with 1cc of MS solution.
Take the quickest colonizing one & G2G that to 10 more.

GLCs aren't as shitty as LCs, but they still suck.
They take more time, have more room for error (contams to come in), and have extra steps vs. Grain to Grains.

I can't remember the last time I touched a syringe.
MS-->agar-->isolate-->master grain jar-->G2G-->G2G-->G2G-->spawn.





:whathesaid:

Beat me to it.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
Trusted Cultivator
OG Cultivator
Re: Syringe or Grain to Grain [Re: sammich]
    #18437293 - 06/18/13 02:25 PM (11 years, 6 months ago)

Quote:

sammich said:
are there any benefits from grain to grain transfers instead of using prints to make spore syringes?




Yes.  Grain to grain transfers are many times faster than starting over from spores.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Offlinesammich
Stranger

Registered: 05/30/13
Posts: 25
Last seen: 11 years, 6 months
Re: Syringe or Grain to Grain [Re: RogerRabbit]
    #18441210 - 06/19/13 07:37 AM (11 years, 6 months ago)

I see.. I'm a bit worried about grain to grain transfers (not grain LC) since I don't have a flow hood or a still air box :/

Extras: Filter Print Post Top
InvisibleTranscendingLife
I Don't Need a Life to Live
Male User Gallery


Registered: 06/09/10
Posts: 21,627
Trusted Cultivator
Re: Syringe or Grain to Grain [Re: sammich]
    #18441425 - 06/19/13 09:06 AM (11 years, 6 months ago)



--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Can one do grain to grain / mycelium transfers via oven tek? none 1,539 4 08/04/03 03:02 PM
by tripndicular
* PC'ing grains and transfering to jars BigJohnson 1,815 11 04/09/03 11:06 AM
by tripndicular
* Grain to Grain kindkesey 2,359 13 03/03/04 07:50 PM
by Anonymous
* Liquidplaya's 99% contam free grain -> X TransfTek liquidplaya 2,155 7 10/25/16 10:59 AM
by Greg
* G2G transfers
( 1 2 all )
kjb1891 33,625 20 12/19/18 11:45 AM
by Nichrome
* grain transfers and spore printing esin 1,333 1 05/22/02 06:12 AM
by angryshroom
* black dot contam in 100% colonized WBS? thisone 2,630 14 10/15/03 04:43 PM
by thisone
* Cake to grain?? azurescens 1,124 5 10/12/04 03:12 PM
by Hamurabi

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
969 topic views. 28 members, 93 guests and 13 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.006 seconds on 12 queries.