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Invisible8ow8
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The moment you realize that it's not mycelium....
    #18436164 - 06/18/13 10:29 AM (11 years, 6 months ago)

After a couple of knocked-up jars showed what I thought was a mix of myc and contams...

(the water's just condensation after being removed from the incubator)

I tried to isolate on agar to clean the contam. I kept grabbing bits of fluffy, white mycelim, as far from the contaminated areas as possible... but it seemed I couldn't get a clean wedge. Each transfer point would start out stringy and beautiful, then develop little white spots that would mature into a lustrous olive green. I transferred four times before I finally realized I was actually growing aspergillus. You can see it even on this tiny, two-day-old wedge. *cries*



Apparently some subspecies of aspergillus have a leading edge that looks very similar to myc... I still can't believe that that wasn't mushies growing in the jars. Sad day, whole syringe lost. :spiderrevenge:  :sad:


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Offlineabeo3073
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Re: The moment you realize that it's not mycelium.... [Re: 8ow8]
    #18436293 - 06/18/13 10:58 AM (11 years, 6 months ago)

Hey did you pressure cook your substrate?

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OfflineKizzle
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Re: The moment you realize that it's not mycelium.... [Re: 8ow8]
    #18436330 - 06/18/13 11:06 AM (11 years, 6 months ago)

Yeah I think we've all been there before :lol:

Even worst are some of these pin molds. If you don't catch them on agar you can colonize a whole jar without knowing even knowing it. That's what used to get me all the time :shakefist:


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Offlineabeo3073
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Re: The moment you realize that it's not mycelium.... [Re: abeo3073]
    #18436350 - 06/18/13 11:11 AM (11 years, 6 months ago)

Did you pressure cook your substrate?

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OfflineSleepyE
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Re: The moment you realize that it's not mycelium.... [Re: abeo3073]
    #18436380 - 06/18/13 11:19 AM (11 years, 6 months ago)

:feelsbadman:


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OfflineJimmyhunter1000
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Re: The moment you realize that it's not mycelium.... [Re: 8ow8]
    #18436478 - 06/18/13 11:42 AM (11 years, 6 months ago)

That looks like little hairs, Myc looks like a much thicker rope on the edge. You've got nothing but mold there.


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Invisible8ow8
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Re: The moment you realize that it's not mycelium.... [Re: SleepyE]
    #18436491 - 06/18/13 11:47 AM (11 years, 6 months ago)

Thanks for commiserating guys.

Kizzle, amazing how rhizomorphic that pin mold looks! At least mine turns green in the jar!

And abeo, yup, sterilized with PC for an hour, used glove box, careful procedure, I'm pretty sure it was in the syringe. It showed up in everything I knocked up, right at the inoculation point (4 jars and 4 plates).


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Invisible8ow8
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Re: The moment you realize that it's not mycelium.... [Re: Jimmyhunter1000]
    #18436551 - 06/18/13 12:00 PM (11 years, 6 months ago)

Quote:

Jimmyhunter1000 said:
That looks like little hairs, Myc looks like a much thicker rope on the edge. You've got nothing but mold there.




Even in the jar? I've had mycelium that looks more or less exactly like that...


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