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Novanity1
Student

Registered: 08/29/10
Posts: 326
Last seen: 9 years, 2 months
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SFD vs. Syringe Filter
#18435373 - 06/18/13 03:17 AM (10 years, 8 months ago) |
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Right now I'm currently using tyvek and syringe filters for GE on my grain lids. Tyvek is total crap because it goes bad after a couple uses. The syringe filters seem like they should work in theory. However, it looks like such a small amount of GE. So far they are working for me but the growth in my grain jars is a bit slow.
SFD seems to be the most popular for lids but I've yet to try it.
Could anyone with experience using both please tell me the pros and cons? 
I tried searching but it was pretty difficult to find threads with the two filters compared.
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TrentBoyett
Aspiring Mycologist



Registered: 11/29/12
Posts: 16,000
Loc: Kazakhstan
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Re: SFD vs. Syringe Filter [Re: Novanity1]
#18435423 - 06/18/13 03:56 AM (10 years, 8 months ago) |
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I have no first hand experience but I have seen a couple people on here say that syringe filters can restrict GE and lead to slow growth.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: SFD vs. Syringe Filter [Re: Novanity1]
#18435452 - 06/18/13 04:17 AM (10 years, 8 months ago) |
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Syringe filters should be used for liquid cultures, not grain jars. They don't provide enough gas exchange for grains, whereas LC's don't need GE. Use the discs for grain jars.
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Quick WBS Prep
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: SFD vs. Syringe Filter [Re: FooMan]
#18435565 - 06/18/13 05:40 AM (10 years, 8 months ago) |
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Depends on what kind of tyvek you're using. The envelope stuff I wouldn't consider reusable but the coverall material is more durable.
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Novanity1
Student

Registered: 08/29/10
Posts: 326
Last seen: 9 years, 2 months
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Re: SFD vs. Syringe Filter [Re: Kizzle]
#18436709 - 06/18/13 12:31 PM (10 years, 8 months ago) |
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Quote:
Kizzle said: Depends on what kind of tyvek you're using. The envelope stuff I wouldn't consider reusable but the coverall material is more durable.
I'm using the coverall tyvek and it get's really gunky after about 5 uses. I've already thrown half of them out.
Fooman, Right now I have 6 grain jars with syringe filters on the jar lid. They are definitely slow and I won't be using them anymore, but do you think the growth will stall before 100%?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: SFD vs. Syringe Filter [Re: FooMan]
#18436727 - 06/18/13 12:35 PM (10 years, 8 months ago) |
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Quote:
FooMan said: Syringe filters should be used for liquid cultures, not grain jars. They don't provide enough gas exchange for grains, whereas LC's don't need GE. Use the discs for grain jars.

110%.
Every single trial run I did with grains and syringe filters stalled out. Too little GE.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: SFD vs. Syringe Filter [Re: Novanity1]
#18436779 - 06/18/13 12:45 PM (10 years, 8 months ago) |
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Quote:
FrankHorrigan said:
Quote:
FooMan said: Syringe filters should be used for liquid cultures, not grain jars. They don't provide enough gas exchange for grains, whereas LC's don't need GE. Use the discs for grain jars.

110%.
Every single trial run I did with grains and syringe filters stalled out. Too little GE.
s
Quote:
Novanity1 said:
Quote:
Kizzle said: Depends on what kind of tyvek you're using. The envelope stuff I wouldn't consider reusable but the coverall material is more durable.
I'm using the coverall tyvek and it get's really gunky after about 5 uses. I've already thrown half of them out.
Fooman, Right now I have 6 grain jars with syringe filters on the jar lid. They are definitely slow and I won't be using them anymore, but do you think the growth will stall before 100%?
I doubt they'll make it.
Notice the difference in hole size of a SFD vs. a Syringe filter:
 
VS
 
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Novanity1
Student

Registered: 08/29/10
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ohhhh noooo I just knocked up 6 different PE jars with the strains I've been isolating on agar. I was really looking forward to this grow. Any suggestions on what I can do if they stall out? Open the lids in and SAB maybe? Replace the lids with proper ones inside an SAB?
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: SFD vs. Syringe Filter [Re: Novanity1]
#18436838 - 06/18/13 12:56 PM (10 years, 8 months ago) |
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Quote:
Novanity1 said: Replace the lids with proper ones inside an SAB?
Yup. Sterilize proper lids & transfer them in the SAB.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Quote:
TranscendingLife said:
Quote:
Novanity1 said: Replace the lids with proper ones inside an SAB?
Yup. Sterilize proper lids & transfer them in the SAB.

Wrap the lids in foil. You'll need a way to get them to your SAB clean.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Quote:
FrankHorrigan said:
Quote:
TranscendingLife said:
Quote:
Novanity1 said: Replace the lids with proper ones inside an SAB?
Yup. Sterilize proper lids & transfer them in the SAB.

Wrap the lids in foil. You'll need a way to get them to your SAB clean.
?
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Couldn't find any others than what he said, im with stupid and what she said.
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Novanity1
Student

Registered: 08/29/10
Posts: 326
Last seen: 9 years, 2 months
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Awesome, that's exactly what I had in mind. Thanks a lot guys!
Any time frame this needs to be done in before the grains are fucked? I would have to wait for the SFD's to be mailed here.
I've got 4 lids with the tyvek filters but for the other 2 I'm not sur what I should do. Could I get away using my old PF tek jar lids and putting micropore tape over the 4 holes?
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: SFD vs. Syringe Filter [Re: Novanity1]
#18436975 - 06/18/13 01:21 PM (10 years, 8 months ago) |
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Order them shits next day. Or use the tyvek lids.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Novanity1
Student

Registered: 08/29/10
Posts: 326
Last seen: 9 years, 2 months
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Definitely ordering some SFD immediately but I don't have money to next day ship it. Tyvek it is for now.
Edited by Novanity1 (06/18/13 02:35 PM)
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Novanity1
Student

Registered: 08/29/10
Posts: 326
Last seen: 9 years, 2 months
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Re: SFD vs. Syringe Filter [Re: Novanity1]
#18437456 - 06/18/13 02:55 PM (10 years, 8 months ago) |
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The reason I went with the syringe filters is because the sponsor I bought them from also sells pre-sterilized rye jars with the same type of filters on the lids. So I assumed it would work no problem.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: SFD vs. Syringe Filter [Re: Novanity1]
#18437464 - 06/18/13 02:56 PM (10 years, 8 months ago) |
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Yes. There are a few sponsors who advertise that. It's old information.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 10 hours
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Re: SFD vs. Syringe Filter [Re: Novanity1]
#18438331 - 06/18/13 06:02 PM (10 years, 8 months ago) |
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Quote:
Novanity1 said:
Quote:
Kizzle said: Depends on what kind of tyvek you're using. The envelope stuff I wouldn't consider reusable but the coverall material is more durable.
I'm using the coverall tyvek and it get's really gunky after about 5 uses. I've already thrown half of them out.
Fooman, Right now I have 6 grain jars with syringe filters on the jar lid. They are definitely slow and I won't be using them anymore, but do you think the growth will stall before 100%?
Were you using the tyvek on the bottom side of the lid?
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Novanity1
Student

Registered: 08/29/10
Posts: 326
Last seen: 9 years, 2 months
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Re: SFD vs. Syringe Filter [Re: Kizzle]
#18438509 - 06/18/13 06:41 PM (10 years, 8 months ago) |
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Nope, topside only. I even put a small layer of silicone on the bottom side.

I do this to prevent the inside from rusting.
Here's the top

It's two 1/4" with a double layer of tyvek over the top. I use double for extra protection just in case the tyvek gets wet. Not sure if the double layer makes a difference in that sense but I know it doesn't restrict GE because I've had some great growth with these lids.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: SFD vs. Syringe Filter [Re: Novanity1]
#18441411 - 06/19/13 09:03 AM (10 years, 8 months ago) |
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-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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