Home | Community | Message Board

MagicBag Grow Bags
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies   Myyco.com APE Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
The Consistancy of Agar & My Processes?
    #18431463 - 06/17/13 10:47 AM (11 years, 6 months ago)

Ello there, a few questions about agar, as i've been doing research on it, gaining knowledge from fellow forum members, and I'm just about ready to give it a shot, but i'm the type of guy who likes to get it right the first time.

Let's see if I've got my head around this: :utterconfusion:

Agar is used in cooking to make gelatin like foods. However, in recent years it's been used as a medium in microbiology because fungi and other bacterias take to it well.  Agar itself has no real nutritional value, but this is why we mix it with a nutrient.

My recipe learned here off shroomery, and cuz it's so accessible to me (i have 5 cats and 1 dog, so animal food is always around my home, and I don't feed them shit either, my wife is a massage therapist, and trades massage for animal food and other stuff with 2 clients of hers that own pet shop, TMI but we get top shelf organic pet food). 

20g Agar agar Powder
20g Any Organic Dog Food (grind to fine in blender)
1000ml Water

PC 45 minutes.

Go to buy petri dishes. Glass ones, so I can PC them, ones without holes in em.

QUESTIONS I'VE BEEN PONDERING ON ABOUT AGAR:  :cool:

1)I hear that as the agar cools down it solidifies. Do you want it to be a hard solid, or does it stay gelatin like? All I've seen it in is pictures.  So then, when do I put culture on agar or spores, as soon as I pour it, or after it solidifies I can throw the culture onto the agar at any time?

2) What are some sterile techniques that people take when dealing with agar...I hear its very clean and easy once you get the hang of it, and it's great for grain masters >> g2g xfers so...How do we make sure no contams get on the agar?

3) Are there lids for the petri dishes, or a way to close it up, leaving a little GE and that's it (just like how a jar colonizes).


4) Why do we grain master to g2g xfer.... why not just throw a wedge in each grain jar....and then spawn those jars to bulk? I don't understand  adding the extra step of xfering it AGAIN into more jars. Unless you're just trying to have many many jars.


5) If myc or anything, grows AROUND the agar and not through it, because theres no oxygen in it... why do we mix neutrient in it, if it's not getting anything from inside the agar anyway...is it simply for the neutrient on the outer sides of the agar that the myc eats off of?


6) Given recent events with trich contamination in all my grains, I have no more culture. I have 4cc's left from 4-5 months ago in my fridge this whole time of Albino A+. (if it's even good still) How long does agar take to germinate and colonize spores? I hear it's only 2-3 days for actual culture.


I'm on the right track, and after this test that i'm running, i'm gonna go full force on agar, cuz it seems the right way to do things. I wanted to share my ideas with you guys and the experts to see if yall had any input on my thought processes and answers for my questions i've been accumulating.

I appreciate any feedback and guides in the right direction. :bearbreakdance:


--------------------

Edited by ConcreteWaves (06/17/13 10:51 AM)

Extras: Filter Print Post Top
OfflineGhost4
Stranger


Registered: 12/28/12
Posts: 455
Last seen: 4 years, 2 months
Re: The Consistancy of Agar & My Processes? [Re: ConcreteWaves]
    #18431478 - 06/17/13 10:54 AM (11 years, 6 months ago)

:threadmonitor:


--------------------

Extras: Filter Print Post Top
OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
Re: The Consistancy of Agar & My Processes? [Re: Ghost4]
    #18431486 - 06/17/13 10:58 AM (11 years, 6 months ago)

Quote:

Ghost4 said:
:threadmonitor:





:whatwhatwhat:


--------------------

Extras: Filter Print Post Top
InvisibleUhurungus
Avoiderer of Bullshit
Male

Registered: 04/20/13
Posts: 838
Re: The Consistancy of Agar & My Processes? [Re: ConcreteWaves]
    #18431513 - 06/17/13 11:08 AM (11 years, 6 months ago)

1. unless you have disposable income, i would suggest plastic dishes

2. many agar recipes have been reported as suitable for fungi growth, yours included.  i personally only have experience with MYA and PDA (you can search for these)  i have found better growth (cubes) on PDA but both have worked fine.

3. i have found that PC'ing @ 15 psi for over 45 minutes has "burned" my media, turning it a much deeper brown than @ 30-45 minutes

4. i have not found my prepared agar media solid, and if i did i would personally throw it out and redo.  it's harder than jello but a bit more "grainy"  i learned this from emptying dishes and transfers

5. after removing from your PC let it cool to the touch before attempting a pour.  also to avoid excess condensation i keep a good eye on it and let it cool when i see the beginning of solidification on the sides of container.  i've found the cooler it is at pour the less buildup of condensation inside after sealing with glad wrap. (i seal all my dishes)

6. innoc the agar after it is fully cooled

7. only sterile tek i've used is SAB and it works well.  most contams i get are from questionable or wild spores/lc etc.  you can't make sure no contams exist.  you can just take steps to ensure less happen.

8. lids fit loose enough for GE.  from what i understand micropore tape and glad wrap allow for GE while blocking outside contams.  i've even read somewhere that there is enough O2 inside to allow proper growth were it to be sealed completely (but i don't know this from experience)

9. i can only theorize on GM->G2G vs. agar->grain, but i'm sure it has something to do with strain continuity.

10. germination times vary based on many factors.  i've had growth in 24 hours and i've had growth in 5 days.  i've found that less is more though.  much faster germ/growth times when using 1 drop solution of choice vs. 3 drops.  i usually do 3 drops in 3 places when i start though.  kind of like a triangle.  but i make sure to not tilt dish mixing solution.  too much water in one area welcomes bacteria i believe.

i'm no expert but i started with agar and i feel i got a HUGE head start by doing so.  keep it up and continue to "DO".  i learn a ton from reading threads here but i've learned as much if not more by actually doing.  theory is just theory until applied!

good luck


--------------------
I'm never 100% on anything, just close enough to risk looking foolish.

Extras: Filter Print Post Top
OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
Re: The Consistancy of Agar & My Processes? [Re: Uhurungus]
    #18431553 - 06/17/13 11:19 AM (11 years, 6 months ago)

Quote:

Uhurungus said:

10. germination times vary based on many factors.  i've had growth in 24 hours and i've had growth in 5 days.  i've found that less is more though.  much faster germ/growth times when using 1 drop solution of choice vs. 3 drops.  i usually do 3 drops in 3 places when i start though.  kind of like a triangle.  but i make sure to not tilt dish mixing solution.  too much water in one area welcomes bacteria i believe.






ty for the quick reply. you think my a+ spores that been in my fridge for 5 months are good still?


--------------------

Extras: Filter Print Post Top
InvisibleUhurungus
Avoiderer of Bullshit
Male

Registered: 04/20/13
Posts: 838
Re: The Consistancy of Agar & My Processes? [Re: ConcreteWaves]
    #18431574 - 06/17/13 11:29 AM (11 years, 6 months ago)

that would be better answered by someone in the know but if i had to guess i'd say

YES!


--------------------
I'm never 100% on anything, just close enough to risk looking foolish.

Extras: Filter Print Post Top
Offlinebotanicalbishop
Male

Registered: 12/22/12
Posts: 93
Loc: the abyss
Last seen: 3 years, 28 days
Re: The Consistancy of Agar & My Processes? [Re: Uhurungus]
    #18431602 - 06/17/13 11:39 AM (11 years, 6 months ago)

Yes the syringe is still good. I have one at the moment that is at the 8 month mark been sitting in the closet, knocked up a few plates last week with it.


--------------------
"Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded."

Abraham Lincoln

Extras: Filter Print Post Top
OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
Re: The Consistancy of Agar & My Processes? [Re: botanicalbishop]
    #18431888 - 06/17/13 12:59 PM (11 years, 6 months ago)

Quote:

botanicalbishop said:
Yes the syringe is still good. I have one at the moment that is at the 8 month mark been sitting in the closet, knocked up a few plates last week with it.




O.O u didnt have to keep it in a fridge? :waaaaaaargh:  i heard they go bad within like 48 hours if kept out of a fridge.


--------------------

Extras: Filter Print Post Top
OfflineGhost4
Stranger


Registered: 12/28/12
Posts: 455
Last seen: 4 years, 2 months
Re: The Consistancy of Agar & My Processes? [Re: ConcreteWaves]
    #18431921 - 06/17/13 01:06 PM (11 years, 6 months ago)

It has been theorized that spores can exist in the cold, dark, vacuum of space :smile:


--------------------

Extras: Filter Print Post Top
OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
Re: The Consistancy of Agar & My Processes? [Re: Uhurungus]
    #18431978 - 06/17/13 01:17 PM (11 years, 6 months ago)

Quote:

Uhurungus said:
1. unless you have disposable income, i would suggest plastic dishes







Can you PC plastic anything? It'll melt >.> So glass petri dishes to autoclave.

Less they make PCable plastic petri dishes.


Question 7:

Let's say I make too much somehow...or I only have like 30 dishes, cuz 20g 20g 1000ml is enough for like 50 dishes.  Can I put w/e agar is left, seal it inside a mason jar, put it in the fridge (does it have to be refridgerated?)...and when I go to use it again, just PC is it rlly hot for 30 min so it liquefies again?


--------------------

Edited by ConcreteWaves (06/17/13 01:19 PM)

Extras: Filter Print Post Top
OfflineGhost4
Stranger


Registered: 12/28/12
Posts: 455
Last seen: 4 years, 2 months
Re: The Consistancy of Agar & My Processes? [Re: ConcreteWaves]
    #18432307 - 06/17/13 02:49 PM (11 years, 6 months ago)

you PC your agar, then pour it in sterile conditions


To wash mine I just run them under soap and hot water, pop them in a boiling pot of water for 30 seconds and then run them in the dishwasher.

I think that's overkill too :smile:


--------------------

Extras: Filter Print Post Top
OfflineBloodKil
Mangler av era mödrar slida
 User Gallery

Registered: 03/16/13
Posts: 920
Last seen: 6 months, 12 days
Re: The Consistancy of Agar & My Processes? [Re: Ghost4]
    #18432374 - 06/17/13 03:01 PM (11 years, 6 months ago)

Just cut the recipe down and make less...  13/13/650. (You will have a bit left over, but not enough to save)

Alternatively you could just make it in small batches and pour a few plates at once as needed...

That said if you really wanted to save your left overs, yes it has been done before...  just leave it in the bottle you cooked it in, toss it in the fridge and re heat when ready to use.  (Dependant on how clean your procedure is though you may end up growing something in your bottle...  While I suppose you might just be able to re pc it, I wouldn't.)

With your pour temp don't let it cool too much as it will solidify as your pouring it...  110°-120°(f) would be your optimal temp...  just leave your dishes stacked when you pour them, and then get a glass or shot glass (dependant on plate size) fill it with a couple inches of water and nuke it till it is boiling.  Put that on the top plate and allow them all to cool..  (you will not have any condensation if you do this)

Also bypass the glad wrap and get yourself a roll of parafilm..  (you'll be GLAD you did...  pun intended)  I have worked with both and found Para film much easier to use, plus that's what you should be using anyway.  A 2"×250' roll is around 20 bucks, but I personally slice mine down the center as I use it (strait down the middle of the big M) and still have plenty to wrap a bit over the top and bottom of the plates without covering too much of the top or the bottom so I can see what's going on better.  (Plus it doubles the amount of plates you can do with a roll)

(As far as spores only surviving 48h...  There has been peeps who left syringes in drawers for years and we're still able to use them.)

Edited by BloodKil (06/17/13 03:16 PM)

Extras: Filter Print Post Top
InvisibleTranscendingLife
I Don't Need a Life to Live
Male User Gallery


Registered: 06/09/10
Posts: 21,627
Trusted Cultivator
Re: The Consistancy of Agar & My Processes? [Re: ConcreteWaves]
    #18432448 - 06/17/13 03:19 PM (11 years, 6 months ago)

Quote:

ConcreteWaves said:


1)I hear that as the agar cools down it solidifies. Do you want it to be a hard solid, or does it stay gelatin like? All I've seen it in is pictures.  So then, when do I put culture on agar or spores, as soon as I pour it, or after it solidifies I can throw the culture onto the agar at any time?




If you're pouring to petris, you have to pour it while it's hot.
Once your PC is back to atmospheric pressure, slowly release the rest of the heat via the toggle weight.
Make sure everything is already prepped & you've already started sterile procedures.
Then go into your clean room & pour your agar in front of your flowhood or in your SAB.
If you're doing no pour agar dishes, let cool in the PC over night.
Professor Pinhead has a good "no pour agar" tek.

Quote:

ConcreteWaves said:

2) What are some sterile techniques that people take when dealing with agar...I hear its very clean and easy once you get the hang of it, and it's great for grain masters >> g2g xfers so...How do we make sure no contams get on the agar?




Same sterile techniques as doing a G2G or anything mycology related.
Agar is great for isolating AWAY from contams.
You'll see the good mycelium, which is what you want to transfer.
It may take a few transfers to clean up a culture.

Quote:

ConcreteWaves said:
3) Are there lids for the petri dishes, or a way to close it up, leaving a little GE and that's it (just like how a jar colonizes).




Use parafilm or Saran Wrap.
They need a bit of GE.

Quote:

ConcreteWaves said:
4) Why do we grain master to g2g xfer.... why not just throw a wedge in each grain jar....and then spawn those jars to bulk? I don't understand  adding the extra step of xfering it AGAIN into more jars. Unless you're just trying to have many many jars.




Grain masters & master slants are a great way to ensure you have a proven good culture.
When I get my culture that I want, I make 2 grain masters.
1 I use immediately & can G2G that to 10 to 10 etc.
The other I keep in the fridge with my master slants (I make about 5 master slants per culture).


Quote:

ConcreteWaves said:
5) If myc or anything, grows AROUND the agar and not through it, because theres no oxygen in it... why do we mix neutrient in it, if it's not getting anything from inside the agar anyway...is it simply for the neutrient on the outer sides of the agar that the myc eats off of?




Agar is a 2D growth. It should not colonize the underside of the petri.
The nutrient is all through the agar. So it'll get the nutrients from the top layer of it.

Quote:

ConcreteWaves said:
6) Given recent events with trich contamination in all my grains, I have no more culture. I have 4cc's left from 4-5 months ago in my fridge this whole time of Albino A+. (if it's even good still) How long does agar take to germinate and colonize spores? I hear it's only 2-3 days for actual culture.




It can take from 2-10 days.
Spores take longer.

On another note, remember that no dog food is organic.
They're not regulated by the FDA, so dog food manufacturers can claim organic when it's not.
Just like "organic" body products.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

Extras: Filter Print Post Top
OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
Re: The Consistancy of Agar & My Processes? [Re: TranscendingLife]
    #18432542 - 06/17/13 03:43 PM (11 years, 6 months ago)

Quote:

TranscendingLife said:


It can take from 2-10 days.
Spores take longer.

On another note, remember that no dog food is organic.
They're not regulated by the FDA, so dog food manufacturers can claim organic when it's not.
Just like "organic" body products.




http://www.redbandannapetfood.com/browse.cfm/nv-prairie-dog-new-zealand-venison-meal-millet-30/4,670.html

This is the dog food I have and gonna throw into the agar mix, what do you think, it will do fine?

Also, i'm going to stick to the pour agar method for now, the no pour agar tek can come later. I got the idea in my head how this whole process is gonna go and pouring it in is absolutely fine.


My process:

13g agar powder
13g crushed fine in blender Dog food (link above)
650ml water

put all into a quart jar, and shake it like crazy!

Make sure lid is not on ALL THE WAY, leave it like when i'm doing my grains, and PC for 30-40 min at 15psi.

(i have 2 PCs)

While PCing agar mix...

Place all petri dishes in PC and PC for 20 min at 15 psi (theyre gonna be brand spankin new, and i'm gonna wash them in the sink with hot water and soap aswell..so i think a PC for 20 min should be fine, ya?

Get my SAB out, germicidal bleach clean it, then throw my cleaned and sterilized petri dishes into it, while wearing alcohol soaked gloves, ya?

Wait til the pressure in the PC goes down to 0, while it's still hot, open the PC with the agar, take it out with a pot holder and bring it into the clean area with my SAB, take off the lid and pour into each petri dish, then quickly cover each dish with a little square of seran wrap.

Wait til they cool off and solidify to gelatin like state room temp:

grab spore syringe and inoculate right through seran wrap (after sterile/sanatize teks like flame the tip wearing gloves etc.


As I take out the syringe from the seran wrap, grab a tiny piece of micropore tape and cover the punctured spot left by the syringe.


What you think of this process? Missing anything? Is it ... right? lol


--------------------

Extras: Filter Print Post Top
InvisibleTranscendingLife
I Don't Need a Life to Live
Male User Gallery


Registered: 06/09/10
Posts: 21,627
Trusted Cultivator
Re: The Consistancy of Agar & My Processes? [Re: ConcreteWaves]
    #18432565 - 06/17/13 03:48 PM (11 years, 6 months ago)

sounds good to me.
you may want to use a little less nutrient in your agar.
I've found less is more with nutrient in agar.

It's going to take a few hours from them to solidify.
Think of making jell-o. It's just like that.

Check your petris. They should come sterile in the plastic sleeve.
Also, watch RR's agar video on www.mushroomvideos.com

Oh, and no pour teks are much simpler than the pour teks.
But, since you have petris I'd go with the pour tek.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

Extras: Filter Print Post Top
OfflineBloodKil
Mangler av era mödrar slida
 User Gallery

Registered: 03/16/13
Posts: 920
Last seen: 6 months, 12 days
Re: The Consistancy of Agar & My Processes? [Re: TranscendingLife]
    #18432766 - 06/17/13 04:30 PM (11 years, 6 months ago)

Only thing I'm confused about is the squares of seran wrap and microphone tape....  Are you not using your petri dish lids? 

I use a gb setup, so maybe I just have less to deal with, but so far I've had had luck with just having ALL my supplies wiped down in a freshly sanitized box (clorox wipes)  I do my pours, unzip the back just enough to get a whisky glass with hot water in for my top plate, let cool and then come back  and lift lids just enough to inoculate/replace lids back when done....  then once every dish I'm using is inoculated I wrap the perimeter with Para film.  (Or gw/sw)

Extras: Filter Print Post Top
OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
Re: The Consistancy of Agar & My Processes? [Re: BloodKil]
    #18432915 - 06/17/13 05:04 PM (11 years, 6 months ago)

Quote:

BloodKil said:
Only thing I'm confused about is the squares of seran wrap and microphone tape....  Are you not using your petri dish lids? 

I use a gb setup, so maybe I just have less to deal with, but so far I've had had luck with just having ALL my supplies wiped down in a freshly sanitized box (clorox wipes)  I do my pours, unzip the back just enough to get a whisky glass with hot water in for my top plate, let cool and then come back  and lift lids just enough to inoculate/replace lids back when done....  then once every dish I'm using is inoculated I wrap the perimeter with Para film.  (Or gw/sw)






i didn't know petris come with lids, i havent bought them yet.


so is it settled i can PC plastic petri dishes?

they should come sterile huh, i thought about that too aye?

if i use the lids they provide, how do i steriley (lol is that a word?) knock up with a syringe? straight through the plastic lid and micropore the top as I "pull out"  :bashful: ?


--------------------

Extras: Filter Print Post Top
Offlinetwistedty
Forcefully Retired
Male User Gallery


Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 4 years, 5 months
Re: The Consistancy of Agar & My Processes? [Re: ConcreteWaves]
    #18432955 - 06/17/13 05:13 PM (11 years, 6 months ago)

no just lift lid up and give it a small drop of solution in a sab

Extras: Filter Print Post Top
OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
Re: The Consistancy of Agar & My Processes? [Re: twistedty]
    #18433000 - 06/17/13 05:21 PM (11 years, 6 months ago)

Right on, i appreciate it. I'm buying the correct powder soon, so - anymore info and experiences yall can throw at me before I give this a go will be much appreciated ^_^ Cant wait to try it out.

So whatcha think of the dog food in that link I posted? Good aye?


--------------------

Extras: Filter Print Post Top
OfflineBloodKil
Mangler av era mödrar slida
 User Gallery

Registered: 03/16/13
Posts: 920
Last seen: 6 months, 12 days
Re: The Consistancy of Agar & My Processes? [Re: ConcreteWaves]
    #18433006 - 06/17/13 05:22 PM (11 years, 6 months ago)

Might as well use plastics Imo..  (relatively cheap and if you break them or get a contam you can just trash it)

You can't pc them, but I have *so far* had luck with just washing by hand and then dropping pan of 1:10 bleach for a bit, sanitizing my gb, and letting them dry face down on a couple clorox wipes.  (Not the best way I'm sure, but I've had a few go through this cycle 3 times now, and have not had any issues yet)

Don't go through the lid though...  just lift the lid at an angle, and inoculate.  I use x plates so I may not have to open as far as you would if your using monos, but the same method should work

Go watch rr' s "lets grow mushrooms" videos...  let's learn agar, strain isolation, and master culture slants are the sections you want to be exact...  The video are well worth their small price and will give you a good start on knowing what's going on.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies   Myyco.com APE Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck


Similar ThreadsPosterViewsRepliesLast post
* Benefits of Agar? celsius 9,660 10 12/01/19 12:36 AM
by wildernessjunkie
* do you let it cool down first using agar? crazytab 2,258 4 01/20/04 06:51 PM
by plura16
* -=Newbies 411 Agar for n00bs=- ThatDaltonGuy 4,416 16 04/27/04 10:02 AM
by ThatDaltonGuy
* sources of agar carnage 2,304 3 08/01/03 08:06 PM
by
* The science of proper nutrients Land_Crab 1,071 6 12/03/04 03:56 PM
by tripndicular
* test tubes agar fungophiliac 3,280 8 06/19/20 09:41 PM
by gorilliam
* jello instead of agar? Anonymous 10,096 14 01/14/18 03:57 PM
by mushpunx
* nutrients tuna 1,924 6 11/15/03 03:00 PM
by enimatpyrt

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,349 topic views. 31 members, 131 guests and 27 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.008 seconds on 14 queries.