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Offlinekeepsake
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Registered: 02/24/13
Posts: 673
Last seen: 9 years, 3 months
Is this contam or am I ok?
    #18431760 - 06/17/13 12:29 PM (11 years, 6 months ago)



I noc'd 15 jars 2 weeks ago and I see very little mycelium spots.
I live in NV now so it's REAAAALLY dry. I need to buy a humidifier for my room.
But is this normal? I cant tell.

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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Is this contam or am I ok? [Re: keepsake]
    #18431802 - 06/17/13 12:40 PM (11 years, 6 months ago)

That slimy wet looking stuff is most likely bacteria :frown:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Offlinekeepsake
Male

Registered: 02/24/13
Posts: 673
Last seen: 9 years, 3 months
Re: Is this contam or am I ok? [Re: FrankHorrigan]
    #18431806 - 06/17/13 12:41 PM (11 years, 6 months ago)

Damn.. they're on almost all the jars.

Gotta toss em now?

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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
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Re: Is this contam or am I ok? [Re: keepsake]
    #18431820 - 06/17/13 12:44 PM (11 years, 6 months ago)

You don't have to, they won't contam your area or anything.

But they won't be colonizing right either, so I would toss and retry.

Did they all go bad from the same syringe? It's probably a dirty syringe if so.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Offlinekeepsake
Male

Registered: 02/24/13
Posts: 673
Last seen: 9 years, 3 months
Re: Is this contam or am I ok? [Re: FrankHorrigan]
    #18431845 - 06/17/13 12:50 PM (11 years, 6 months ago)

15 jars w 3 different syringes.
I was pretty sterile. I think I forgot to wash the jars before using them.
I used them straight from the package. Damn it.
Just ordered 4 more syringes. Gotta try again. Thanks man.

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InvisibleThadeous
On the path

Registered: 08/02/11
Posts: 1,101
Re: Is this contam or am I ok? [Re: keepsake]
    #18431852 - 06/17/13 12:52 PM (11 years, 6 months ago)

What was your sterilization procedure on the jars?  That could cause an issue if not up to par.

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Offlinekeepsake
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Registered: 02/24/13
Posts: 673
Last seen: 9 years, 3 months
Re: Is this contam or am I ok? [Re: Thadeous]
    #18431860 - 06/17/13 12:53 PM (11 years, 6 months ago)

I just forgot to wash them before I put the substrate in & steamed in pot.

As for the inoculation process; I did everything up to par.
Glove box, gloves, alcohol, flamed needle w lighter, long sleeve shirt, etc.

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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Is this contam or am I ok? [Re: Thadeous]
    #18431864 - 06/17/13 12:54 PM (11 years, 6 months ago)

Sounds like your syringes may have all been dirty.

Have you had issues like this previously?

I would recommend tyvek wristsleeves for working in the SAB, as well as gloves.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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InvisibleThadeous
On the path

Registered: 08/02/11
Posts: 1,101
Re: Is this contam or am I ok? [Re: FrankHorrigan]
    #18431886 - 06/17/13 12:59 PM (11 years, 6 months ago)

Forgetting to wash them shouldn't be an issue if sterilized properly.  How long were they steamed?  Did you do fractional sterilization?

I guess I have been lucky with my syringes; haven't had a dirty one yet.  It is certainly either the syringes or sterilization that is the issue, methinks.

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Offlinekeepsake
Male

Registered: 02/24/13
Posts: 673
Last seen: 9 years, 3 months
Re: Is this contam or am I ok? [Re: Thadeous]
    #18432058 - 06/17/13 01:38 PM (11 years, 6 months ago)

I sterilized on a low bubble for 90mins.
I will be sure to use wrist sleeves next time.
Thanks fellas.
I flamed the needle between each jars w a lighter.

I'm thinking it was the syringes. They were 4 weeks old and flown in a luggage bag from KY to NV (I just moved).

Oh well.. I'll start over this weekend.
My first grow was a huge success.. this is my 2nd grow in a new environment.
It's all good! The waiting game isnt nothing new to me at this point hehe.

But if anyone lives in the LV area.. hit me up. :wink:

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InvisibleThadeous
On the path

Registered: 08/02/11
Posts: 1,101
Re: Is this contam or am I ok? [Re: keepsake]
    #18432158 - 06/17/13 02:09 PM (11 years, 6 months ago)

Sounds accurate to me.  Best of luck with your next grow then!  Glad to see you've already learned patience.. It appears quite difficult for some :smile:

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