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keepsake


Registered: 02/24/13
Posts: 673
Last seen: 9 years, 3 months
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Is this contam or am I ok?
#18431760 - 06/17/13 12:29 PM (11 years, 6 months ago) |
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I noc'd 15 jars 2 weeks ago and I see very little mycelium spots. I live in NV now so it's REAAAALLY dry. I need to buy a humidifier for my room. But is this normal? I cant tell.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Is this contam or am I ok? [Re: keepsake]
#18431802 - 06/17/13 12:40 PM (11 years, 6 months ago) |
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That slimy wet looking stuff is most likely bacteria
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keepsake


Registered: 02/24/13
Posts: 673
Last seen: 9 years, 3 months
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Damn.. they're on almost all the jars.
Gotta toss em now?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Is this contam or am I ok? [Re: keepsake]
#18431820 - 06/17/13 12:44 PM (11 years, 6 months ago) |
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You don't have to, they won't contam your area or anything.
But they won't be colonizing right either, so I would toss and retry.
Did they all go bad from the same syringe? It's probably a dirty syringe if so.
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keepsake


Registered: 02/24/13
Posts: 673
Last seen: 9 years, 3 months
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15 jars w 3 different syringes. I was pretty sterile. I think I forgot to wash the jars before using them. I used them straight from the package. Damn it. Just ordered 4 more syringes. Gotta try again. Thanks man.
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Thadeous
On the path

Registered: 08/02/11
Posts: 1,101
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Re: Is this contam or am I ok? [Re: keepsake]
#18431852 - 06/17/13 12:52 PM (11 years, 6 months ago) |
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What was your sterilization procedure on the jars? That could cause an issue if not up to par.
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keepsake


Registered: 02/24/13
Posts: 673
Last seen: 9 years, 3 months
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Re: Is this contam or am I ok? [Re: Thadeous]
#18431860 - 06/17/13 12:53 PM (11 years, 6 months ago) |
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I just forgot to wash them before I put the substrate in & steamed in pot.
As for the inoculation process; I did everything up to par. Glove box, gloves, alcohol, flamed needle w lighter, long sleeve shirt, etc.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Is this contam or am I ok? [Re: Thadeous]
#18431864 - 06/17/13 12:54 PM (11 years, 6 months ago) |
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Sounds like your syringes may have all been dirty.
Have you had issues like this previously?
I would recommend tyvek wristsleeves for working in the SAB, as well as gloves.
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Thadeous
On the path

Registered: 08/02/11
Posts: 1,101
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Forgetting to wash them shouldn't be an issue if sterilized properly. How long were they steamed? Did you do fractional sterilization?
I guess I have been lucky with my syringes; haven't had a dirty one yet. It is certainly either the syringes or sterilization that is the issue, methinks.
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keepsake


Registered: 02/24/13
Posts: 673
Last seen: 9 years, 3 months
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Re: Is this contam or am I ok? [Re: Thadeous]
#18432058 - 06/17/13 01:38 PM (11 years, 6 months ago) |
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I sterilized on a low bubble for 90mins. I will be sure to use wrist sleeves next time. Thanks fellas. I flamed the needle between each jars w a lighter.
I'm thinking it was the syringes. They were 4 weeks old and flown in a luggage bag from KY to NV (I just moved).
Oh well.. I'll start over this weekend. My first grow was a huge success.. this is my 2nd grow in a new environment. It's all good! The waiting game isnt nothing new to me at this point hehe.
But if anyone lives in the LV area.. hit me up.
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Thadeous
On the path

Registered: 08/02/11
Posts: 1,101
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Re: Is this contam or am I ok? [Re: keepsake]
#18432158 - 06/17/13 02:09 PM (11 years, 6 months ago) |
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Sounds accurate to me. Best of luck with your next grow then! Glad to see you've already learned patience.. It appears quite difficult for some
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