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Offlinelilguy
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Registered: 05/20/13
Posts: 51
Loc: VT
Last seen: 10 years, 1 month
Inoculation Sterilization
    #18386919 - 06/08/13 08:47 AM (11 years, 7 months ago)

When inoculating it seems it's common practice to heat up the syringe then wipe it with alcohol before injecting the spores into pf tek jars.  I however have done it with alcohol first then flame letting it cool.  Is this way still safe?  I didn't use the sponge trick, but the space I innoc. in was wiped down with 70% alcohol, as was my arms/hands while wearing a resperator after taking a shower.  Do you guys think I have a good chance of being okay doing it this way?

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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
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Re: Inoculation Sterilization [Re: lilguy]
    #18386925 - 06/08/13 08:50 AM (11 years, 7 months ago)

Quote:

lilguy said:
When inoculating it seems it's common practice to heat up the syringe then wipe it with alcohol before injecting the spores into pf tek jars.



Not really, never wipe a flame sterilized tool with an unsterile substance like alcohol.

Flame the needle until red hot between each jar....you can optionally wipe the needle before flaming, but never wipe after flaming.

Quote:

lilguy said:
I however have done it with alcohol first then flame letting it cool.  Is this way still safe? 



Yes.

Quote:

lilguy said:
Do you guys think I have a good chance of being okay doing it this way?



Yes.

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Offlinecubenpete
Aminita good excuse
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Registered: 03/13/12
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Re: Inoculation Sterilization [Re: PussyFart]
    #18386971 - 06/08/13 09:08 AM (11 years, 7 months ago)

OK, this is sounding a little profane... :laugh2:

I just heat the needle till red hot, then sacrifice some solution till I hear the sizzle (pushing a little through the syringe),I then inject some solution into a jar, then repeat. It maybe wasteful, but I know that my shit is sterile.

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Offlinelilguy
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Registered: 05/20/13
Posts: 51
Loc: VT
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Re: Inoculation Sterilization [Re: cubenpete]
    #18387013 - 06/08/13 09:24 AM (11 years, 7 months ago)

Quote:

cubenpete said:
OK, this is sounding a little profane... :laugh2:

I just heat the needle till red hot, then sacrifice some solution till I hear the sizzle (pushing a little through the syringe),I then inject some solution into a jar, then repeat. It maybe wasteful, but I know that my shit is sterile.




How does it sound profane?

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Offlinelilguy
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Registered: 05/20/13
Posts: 51
Loc: VT
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Re: Inoculation Sterilization [Re: PussyFart]
    #18387025 - 06/08/13 09:27 AM (11 years, 7 months ago)

Quote:

Notahacker420 said:
Quote:

lilguy said:
When inoculating it seems it's common practice to heat up the syringe then wipe it with alcohol before injecting the spores into pf tek jars.



Not really, never wipe a flame sterilized tool with an unsterile substance like alcohol.

Flame the needle until red hot between each jar....you can optionally wipe the needle before flaming, but never wipe after flaming.

Quote:

lilguy said:
I however have done it with alcohol first then flame letting it cool.  Is this way still safe? 



Yes.

Quote:

lilguy said:
Do you guys think I have a good chance of being okay doing it this way?



Yes.




Thanks man, put some confidence in me.

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OfflinePussyFart
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Re: Inoculation Sterilization [Re: lilguy]
    #18387027 - 06/08/13 09:28 AM (11 years, 7 months ago)

Quote:

lilguy said:
put some confidence in me.



Ok.... :doggystyle:

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OfflineTrippy_Penguin
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Registered: 03/18/12
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Re: Inoculation Sterilization [Re: lilguy]
    #18387029 - 06/08/13 09:28 AM (11 years, 7 months ago)

Wondering the same thing... Sounds like you'll be fine. Good luck with your grow!

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Offlinelilguy
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Registered: 05/20/13
Posts: 51
Loc: VT
Last seen: 10 years, 1 month
Re: Inoculation Sterilization [Re: PussyFart]
    #18387327 - 06/08/13 11:04 AM (11 years, 7 months ago)

Quote:

Notahacker420 said:
Quote:

lilguy said:
put some confidence in me.



Ok.... :doggystyle:



:sexymeow:

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Offlineveda_sticks
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Re: Inoculation Sterilization [Re: lilguy]
    #18387357 - 06/08/13 11:12 AM (11 years, 7 months ago)

alcohol does not sterilise, and it wont get what could have gotten inside the needle.

Alcohol is merely a sanitiser. Its usefull for wiping injection sites down, jars when doing g2g and your gloves hands and surfaces infront of a flowhood.

Flaming your needle steriles the outside of the needle, and the inside. It helps prevent cross contamination incase there was something in 1 of your jars that somehow got there after sterilisation, and also from when moving your syringe around between jars.

Work in a still air box
wear gloves
long sleeves (tyvek sleeves are good for this)
wash gloved hands every few jars

wipe jar lid with ipa
flame needle then inoc.
Go to next jar wipe injection site , flame , inoc

etc etc

high success rates with not very much effort. its awkward at first working in a SAB but its worth it.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinelilguy
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Registered: 05/20/13
Posts: 51
Loc: VT
Last seen: 10 years, 1 month
Re: Inoculation Sterilization [Re: veda_sticks]
    #18431608 - 06/17/13 11:40 AM (11 years, 6 months ago)

Quote:

veda_sticks said:
alcohol does not sterilise, and it wont get what could have gotten inside the needle.

Alcohol is merely a sanitiser. Its usefull for wiping injection sites down, jars when doing g2g and your gloves hands and surfaces infront of a flowhood.

Flaming your needle steriles the outside of the needle, and the inside. It helps prevent cross contamination incase there was something in 1 of your jars that somehow got there after sterilisation, and also from when moving your syringe around between jars.

Work in a still air box
wear gloves
long sleeves (tyvek sleeves are good for this)
wash gloved hands every few jars

wipe jar lid with ipa
flame needle then inoc.
Go to next jar wipe injection site , flame , inoc

etc etc

high success rates with not very much effort. its awkward at first working in a SAB but its worth it.




Thanks for the advice man.  Didn't use a glove box, but the room was very clean with no draft and everything was wipped down with a bleach solution before.  Been two weeks and I don't see any contam out of the 30 jars.  Most are about 60% complete.  On the bottom of some though it's mucky and I don't know if it will colonize.  What should I do in the case it colonizes everything but the bottom?

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OfflinecronicrMFacebook
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Registered: 08/07/11
Posts: 62,899
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Re: Inoculation Sterilization [Re: lilguy]
    #18431633 - 06/17/13 11:48 AM (11 years, 6 months ago)

doesn't sound good, sounds like bacteria bro


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlineslap and tickle
jonny fungi
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Registered: 05/29/13
Posts: 17
Loc: your back yard
Last seen: 11 years, 3 months
Re: Inoculation Sterilization [Re: cronicr]
    #18431691 - 06/17/13 12:07 PM (11 years, 6 months ago)

How large of jars are you using? If half gallon they could be going into aerobic decomp.  Also try tipping them at 45% angle they may not be getting enough gas exchange.

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