Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 2 months, 7 days
first time with agar to grain xfer, need some advice
    #18429715 - 06/16/13 08:57 PM (10 years, 7 months ago)

I have what appears to be a healthy agar plate.  It's growing slowly but it's rhizomorphic.  It's a little bigger than a quarter.  I can't get a decent pic because of the condensation in the plate.  I want to get some rye berries in a half-pint jar with a sfd so I can make a grain master.  I'm planning to make 4 jars and to cut the thing in 4 pieces.  I don't have a scalpel yet and was just going to sterilize(pc) a spoon or a knife.  This would be in a glove box.  If it works out I'd like to get some 2lb bags pced and g2g them from the master jars.

I don't have many or any quart jars that I can use but have plenty of 1/2 pints ready to go.  I have some large bags from MadSeason with filter patches.

helpful comments? suggestions? 


Extras: Filter Print Post Top
Offlinetwistedty
Forcefully Retired
Male User Gallery


Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
Re: first time with agar to grain xfer, need some advice [Re: invitro]
    #18429750 - 06/16/13 09:03 PM (10 years, 7 months ago)

you can us an exacto knife or utility knife, thinner blades are better since they get red hot faster.  and i would really really recommend to try and get quart jars of grain.


Extras: Filter Print Post Top
OfflineChris893
The Lurker

Registered: 03/01/13
Posts: 209
Last seen: 5 years, 8 months
Re: first time with agar to grain xfer, need some advice [Re: twistedty]
    #18429769 - 06/16/13 09:08 PM (10 years, 7 months ago)

Agreed, just go buy some quarts like $12 for 6 or 12. Can't remember. Transfer to agar as well as grains to be safe


Extras: Filter Print Post Top
Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 2 months, 7 days
Re: first time with agar to grain xfer, need some advice [Re: twistedty]
    #18429789 - 06/16/13 09:12 PM (10 years, 7 months ago)

I'd almost rather pc 4 spoons, wrapping each one in foil individually and putting that in the glove box.  flame sterilizing is very difficult for me in a glove box.  I have thick gloves with long sleeves.  I'll do whatever it takes to make this work but spoons seem easier if I'm just doing 4 xfers.  Yes, no?

I do have a quart jar or two I could use.  I'll give that some thought.
:howveryexciting:


Extras: Filter Print Post Top
Offlinetwistedty
Forcefully Retired
Male User Gallery


Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
Re: first time with agar to grain xfer, need some advice [Re: invitro]
    #18429793 - 06/16/13 09:14 PM (10 years, 7 months ago)

thats why i dont use glove sleeves, so i flame sterilize out of box on my alcohol lamp and bring back into sab while still red hot.


Extras: Filter Print Post Top
Offlinetwistedty
Forcefully Retired
Male User Gallery


Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
Re: first time with agar to grain xfer, need some advice [Re: twistedty]
    #18429794 - 06/16/13 09:14 PM (10 years, 7 months ago)

if you do 2 jars just use 2 wedges per jar if youre gonna 1/4 the dish


Extras: Filter Print Post Top
Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 2 months, 7 days
Re: first time with agar to grain xfer, need some advice [Re: twistedty]
    #18430254 - 06/16/13 11:30 PM (10 years, 7 months ago)

I soaked 3 cups worth of rye berries just now, because I have 3 quart jars I can use.  I was thinking though, couldn't I use a large grain bag instead and put a wedge into 2 lbs of grain?  Would that cause problems?


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* grain transfers bigj2 876 5 06/05/03 09:07 PM
by hyper_dermic
* Grow Advice
( 1 2 all )
mmmbeefy 2,673 24 01/20/04 07:58 AM
by thechemist
* Cake to grain?? azurescens 1,095 5 10/12/04 03:12 PM
by Hamurabi
* Agar and growing that white stuff! pj541 2,517 5 10/15/01 04:06 PM
by pj541
* Innoculating with agar wedge observations homebrew 3,513 4 03/01/02 08:00 AM
by homebrew
* Agar to substrate xfer cucumelero 671 3 10/08/04 11:08 PM
by hyphae
* Pastywhyte's Easy Agar Tek
( 1 2 3 4 ... 375 376 )
PastywhyteMDiscord 796,693 7,515 02/04/24 08:43 PM
by elasticaltiger
* Agar ;)
( 1 2 all )
SCIOpenEyedDream 7,190 25 09/01/04 09:58 AM
by fastfred

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
367 topic views. 27 members, 181 guests and 42 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.008 seconds on 14 queries.