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frommoon
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Opinions wanted - viability of continuous grain to grain xfers
#5797924 - 06/28/06 12:04 AM (17 years, 7 months ago) |
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I read in Stamets that strains will weaken over time. Lets say I start a rice cake from spores then use it to inoculate a bag of grain spawn. From there I always use a little spawn from the bags to do grain xfers to start new bags (the rest goes to trays). I continue to do this with subsequent grain xfers. When do I have to start worrying about the strain going bad or no longer being worth cultivating? How many continuous xfers is normally viable? thanks.
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faceyneck
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: frommoon]
#5797956 - 06/28/06 12:11 AM (17 years, 7 months ago) |
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From what I have read on this site, you shouldn't have to worry about viability until after several crops, not several xfers.
-------------------- Anything posted here, is total bullshit. My Meyers-Briggs Personality: INTJ New growers, or anyone else just needing help; I'm always glad to help right here.
We give cultivation advice here. AMU Q & A - We're glad to help My Doggy Door Greenhouse! First Ever Shmuvbox Tek! Do Manure Right!
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frommoon
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: faceyneck]
#5797991 - 06/28/06 12:20 AM (17 years, 7 months ago) |
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What do you mean several crops - taking spores from later crops? I mean how long can you just keep producing grain spawn that came from the same origin.
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faceyneck
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: frommoon]
#5798031 - 06/28/06 12:32 AM (17 years, 7 months ago) |
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No, not spores from later crops. I mean taking a piece from one or several of the mushrooms from the first crop, and making a slurry to be used to spawn more substrate. You can also just take a chunk of the mycelium before, during, or after your flushes and mix it with sterile water and inoculate more spawn, although its a way better idea to just use a piece of a mushroom and make a slurry. Eventually, that strain will wear down, yes, but it won't be until you've done this several times over (several crops later, maybe even years later. I don't know, that is just what I've read.)
How long?
How long is a piece of string?
You'll just have to keep doing it over and over again until it doesn't work anymore, or slows down until it isn't worth your time anymore.
Read this tek: http://www.shroomery.org/index.php/par/7923
Hope that helps.
-------------------- Anything posted here, is total bullshit. My Meyers-Briggs Personality: INTJ New growers, or anyone else just needing help; I'm always glad to help right here.
We give cultivation advice here. AMU Q & A - We're glad to help My Doggy Door Greenhouse! First Ever Shmuvbox Tek! Do Manure Right!
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frommoon
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: faceyneck]
#5798069 - 06/28/06 12:50 AM (17 years, 7 months ago) |
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I do appeciate your input but I don't think that Stamets was at all refering to using slurry from the crop in regards to the decay of the strain. He was speaking of grain spawn masters and such. As far just doing it and finding out for myself - well that is a good way to learn - but this forum is to help those who have treaded that ground before and help others become more efficient and knowledgeable without having to make the same mistakes. thanks again though. f
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fastfred
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: frommoon]
#5798131 - 06/28/06 01:13 AM (17 years, 7 months ago) |
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It's an interesting question. I'm hoping some people will post their experiences.
I would say that it depends on the strain and your technique. I'm sure there are many ways to speed up or slow down the process. It would be nice to have a general idea though.
-FF
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CoolMojo
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: fastfred]
#5798380 - 06/28/06 04:03 AM (17 years, 7 months ago) |
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well what your doing is splitting the mycelium off and fruiting one portion and keeping the other in a vegetative state. This is just conjecture on my part but given that mycelium can continue to grow in a vegetative state for years (and in some cases many decades) I can't really see how it would be a problem. But even if it is, I don't think it is something you would have tow worry about in the scope we're talking about here.
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Chris893
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: frommoon]
#18429812 - 06/16/13 09:20 PM (10 years, 7 months ago) |
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The question is when will senescence take affect from transfers? It's interesting. You should do an experiment continuously expanding jars and fruiting each time recording biological efficiency, quality of fruits, resistance to contams, etc, with every expansion to see how far the mycelium will go before it not longer fruits and eventually dies. I may do something like this...
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twistedty
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: Chris893]
#18429832 - 06/16/13 09:23 PM (10 years, 7 months ago) |
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thread is 6 years old bro. and you can usually visually tell when the myc is getting weak from senescence
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Chris893
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: twistedty]
#18429886 - 06/16/13 09:37 PM (10 years, 7 months ago) |
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Damn, why was it at the top of the page? I didn't get a thread revival warning either. Guess he would've had time to do the experiment by now lol
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twistedty
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: Chris893]
#18429890 - 06/16/13 09:37 PM (10 years, 7 months ago) |
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Quote:
Chris893 said: Damn, why was it at the top of the page? I didn't get a thread revival warning either. Guess he would've had time to do the experiment by now lol
lol most likely
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Psilicon
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: twistedty]
#18429996 - 06/16/13 10:04 PM (10 years, 7 months ago) |
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While we're all here, though, senescence can be countered by switching to a different substrate. Any different grain or different agar will do.
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twistedty
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: Psilicon]
#18430034 - 06/16/13 10:15 PM (10 years, 7 months ago) |
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Quote:
van der griegen said: While we're all here, though, senescence can be countered by switching to a different substrate. Any different grain or different agar will do.
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Psilicon
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Re: Opinions wanted - viability of continuous grain to grain xfers [Re: twistedty]
#18430052 - 06/16/13 10:19 PM (10 years, 7 months ago) |
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