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Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
first time with agar to grain xfer, need some advice
    #18429715 - 06/16/13 08:57 PM (11 years, 6 months ago)

I have what appears to be a healthy agar plate.  It's growing slowly but it's rhizomorphic.  It's a little bigger than a quarter.  I can't get a decent pic because of the condensation in the plate.  I want to get some rye berries in a half-pint jar with a sfd so I can make a grain master.  I'm planning to make 4 jars and to cut the thing in 4 pieces.  I don't have a scalpel yet and was just going to sterilize(pc) a spoon or a knife.  This would be in a glove box.  If it works out I'd like to get some 2lb bags pced and g2g them from the master jars.

I don't have many or any quart jars that I can use but have plenty of 1/2 pints ready to go.  I have some large bags from MadSeason with filter patches.

helpful comments? suggestions? 

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Offlinetwistedty
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Registered: 07/01/12
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Re: first time with agar to grain xfer, need some advice [Re: invitro]
    #18429750 - 06/16/13 09:03 PM (11 years, 6 months ago)

you can us an exacto knife or utility knife, thinner blades are better since they get red hot faster.  and i would really really recommend to try and get quart jars of grain.

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OfflineChris893
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Registered: 03/01/13
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Re: first time with agar to grain xfer, need some advice [Re: twistedty]
    #18429769 - 06/16/13 09:08 PM (11 years, 6 months ago)

Agreed, just go buy some quarts like $12 for 6 or 12. Can't remember. Transfer to agar as well as grains to be safe

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Offlineinvitro


Registered: 05/03/13
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Re: first time with agar to grain xfer, need some advice [Re: twistedty]
    #18429789 - 06/16/13 09:12 PM (11 years, 6 months ago)

I'd almost rather pc 4 spoons, wrapping each one in foil individually and putting that in the glove box.  flame sterilizing is very difficult for me in a glove box.  I have thick gloves with long sleeves.  I'll do whatever it takes to make this work but spoons seem easier if I'm just doing 4 xfers.  Yes, no?

I do have a quart jar or two I could use.  I'll give that some thought.
:howveryexciting:

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Offlinetwistedty
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Re: first time with agar to grain xfer, need some advice [Re: invitro]
    #18429793 - 06/16/13 09:14 PM (11 years, 6 months ago)

thats why i dont use glove sleeves, so i flame sterilize out of box on my alcohol lamp and bring back into sab while still red hot.

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Offlinetwistedty
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Re: first time with agar to grain xfer, need some advice [Re: twistedty]
    #18429794 - 06/16/13 09:14 PM (11 years, 6 months ago)

if you do 2 jars just use 2 wedges per jar if youre gonna 1/4 the dish

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Offlineinvitro


Registered: 05/03/13
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Re: first time with agar to grain xfer, need some advice [Re: twistedty]
    #18430254 - 06/16/13 11:30 PM (11 years, 6 months ago)

I soaked 3 cups worth of rye berries just now, because I have 3 quart jars I can use.  I was thinking though, couldn't I use a large grain bag instead and put a wedge into 2 lbs of grain?  Would that cause problems?

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