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eddieblake
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Registered: 10/12/09
Posts: 183
Last seen: 9 months, 5 days
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mold or brusing?
#18426529 - 06/16/13 07:37 AM (11 years, 6 months ago) |
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This jar had the best growth out of 12 jars of this batch. It got to around 40 to 50%, i gave it a good shake. two days later, when it grew back, it looked like this.

I don't understand why it looked so good then went so wrong.
-------------------- Member of the cheap bastards club
337 am
The Minor League Paintball
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Chris893
The Lurker

Registered: 03/01/13
Posts: 209
Last seen: 6 years, 7 months
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That jar appears to be full of contams and should be disposed of. Your jar is too full btw, try again using less grains in the jar. If your filter became wet at any point, contams could have entered your jar.
If that is a cake of sorts, there is no need to shake. It only would introduce contams by disturbing the dry vermiculite contam barrier.
What filter material are you using? Is that a cake or grain spawn? What was your sterilization temp and times?
I see you have another jar with foil on it. Foil impedes colonization, so unless that jar is fresh out the pc, I suggest removing it.
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shroomiin
ʂɧɽɸɵɱɨʝɳ



Registered: 12/24/07
Posts: 2,494
Loc: Zone 6
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Re: mold or brusing? [Re: Chris893]
#18426709 - 06/16/13 09:00 AM (11 years, 6 months ago) |
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contam
-------------------- ʋɳʛɼɑʈɛʄʋɭɖɛɑɗ
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: mold or brusing? [Re: shroomiin]
#18426739 - 06/16/13 09:11 AM (11 years, 6 months ago) |
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That is nasty bacteria. The shake distributed it, that jar probably smells nasty. Will need more info on your procedures in order to tell you where you might have gone wrong.
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eddieblake
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Registered: 10/12/09
Posts: 183
Last seen: 9 months, 5 days
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It's wbs, pc'ed for a hour. I assume at 15 psi, not sure though. it's an electric pc, i can't find anywhere online that notes the psi. I used tyvek as a filter above lid and under the ring. Foil was added before pc'ing. The fliter did get wet, it was damp when i pulled the foil off to take the pic. The grain looks wet in pics but not bad in person. maybe my eyes are bad. I use a sab for any inoculating. I inoculated threw the tyvek, immediately used micropore tape over holes. jars were stored a small gh with 12 hour grow light cycle.
Thanks for the help guys.
-------------------- Member of the cheap bastards club
337 am
The Minor League Paintball
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Quote:
eddieblake said: It's wbs, pc'ed for a hour. I assume at 15 psi, not sure though.
This is probably your biggest problem. Should PC for at least 90 min and if I was not sure if it was getting to 15psi I would do 2 hours for sure. You should let the jars cool in the PC to avoid the condensation getting the filter material wet, just remove when pressure drops to shake and then put them back in to finish cooling. Grains should be dry to the touch, so be sure they are well drained. Also be sure to remove the foil after inoculation, all that keeping it on is going to accomplish is to restrict GE.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 24 minutes, 22 seconds
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 hows it going pasty!
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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eddieblake
Stranger



Registered: 10/12/09
Posts: 183
Last seen: 9 months, 5 days
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Quote:
Pastywhyte said:
Quote:
eddieblake said: It's wbs, pc'ed for a hour. I assume at 15 psi, not sure though.
This is probably your biggest problem. Should PC for at least 90 min and if I was not sure if it was getting to 15psi I would do 2 hours for sure. You should let the jars cool in the PC to avoid the condensation getting the filter material wet, just remove when pressure drops to shake and then put them back in to finish cooling. Grains should be dry to the touch, so be sure they are well drained. Also be sure to remove the foil after inoculation, all that keeping it on is going to accomplish is to restrict GE.
Thank you!
-------------------- Member of the cheap bastards club
337 am
The Minor League Paintball
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: mold or brusing? [Re: cronicr]
#18426931 - 06/16/13 10:07 AM (11 years, 6 months ago) |
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Pretty good, gotta busy morning ahead. PC some rye, pasteurizing some sub, little agar work. Gonna be a good day
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 24 minutes, 22 seconds
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Quote:
Pastywhyte said: Pretty good, gotta busy morning ahead. PC some rye, pasteurizing some sub, little agar work. Gonna be a good day 
my plans to the T as well got some pan myc thats begging for a transfer EDIT:forgot about my sig, i should probly remove that soon lol
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
Edited by cronicr (06/16/13 10:12 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: mold or brusing? [Re: cronicr]
#18426960 - 06/16/13 10:15 AM (11 years, 6 months ago) |
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Quote:
cronicr said: EDIT:forgot about my sig, i should probly remove that soon lol
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eddieblake
Stranger



Registered: 10/12/09
Posts: 183
Last seen: 9 months, 5 days
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Quote:
6DEA6 said:
Quote:
eddieblake said: This jar had the best growth out of 12 jars of this batch. It got to around 40 to 50%, i gave it a good shake. two days later, when it grew back, it looked like this.

I don't understand why it looked so good then went so wrong.
Gross, toss that to the PEN 
Pen? Oh i get it. That's a jail reference? well, what is the mandatory sentence for growing oysters? Blues and kings. If i can get high yields i might even start selling them at the local market too! I bet that's even more jail time.
-------------------- Member of the cheap bastards club
337 am
The Minor League Paintball
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Quote:
eddieblake said: Pen? Oh i get it. That's a jail reference? well, what is the mandatory sentence for growing oysters? Blues and kings. If i can get high yields i might even start selling them at the local market too! I bet that's even more jail time.
6DEA6 has been trolling hard and trying to derail threads. Don't listen to him cause at the rate he's going, he shall be banned soon.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 24 minutes, 22 seconds
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i give him a few more hours of log time tops
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: mold or brusing? [Re: cronicr]
#18427259 - 06/16/13 11:39 AM (11 years, 6 months ago) |
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Quote:
cronicr said: i give him a few more hours of log time tops
We should start a pool, I gotta fresh Figi print that say's he will be banned by 3:25 pm MST
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