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OfflineThe Pope
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Registered: 06/09/13
Posts: 5
Last seen: 9 years, 7 months
Contaminated Syringe?
    #18424336 - 06/15/13 05:44 PM (11 years, 7 months ago)

Hello guys,
First off, I'd like to say hi, I've been a lurker for a while now and am astounded by the quality of information given here. Now here's my scenario: I ordered 2 spore syringes from a reputable spore vendor (a shroomery sponsor). I then sterilized 12 pf tek jars via fractional sterilization. For inoculation, I did not use a flowhood, but rather a glovebox in a small room, both of which had been thoroughly disinfected. I used 1cc of inoculant per jar (each jar had 2 inoculation holes). I knocked up 5 jars of golden teacher, and 5 more of the Costa Rican strain, leaving 2 jars empty, so to test the effectiveness of my sterilization. 7 days later, all the Costa Rican jars have germinated and are healthy. I then noticed some green appearing in some of my golden teachers, so I promptly quarantined them. About 10 days later, trichoderma was seen in all of my GT jars, but any growth remained SOLEY at the inoculation points. I checked all my Costa Rican jars, and they remain unaffected. My two jars I kept as a control show NO signs of ANY growth whatsoever, so I am beginning to think it must have been a contaminated syringe. What do you guys think? I don't want to blame a failure on the vendor, but I'm thinking this could be the case. Thanks guys for any opinions.

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OfflineKizzle
Misanthrope
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Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
Trusted Cultivator
Re: Contaminated Syringe? [Re: The Pope]
    #18424353 - 06/15/13 05:52 PM (11 years, 7 months ago)

Syringe would be my guess all things considered. Even decent vendors can send contaminated syringes occasionally but they'll usually send a replacement for free.

Steaming for 90 minutes is enough to sterilize PF jars. No need for a pressure cooker or any other method unless you're working with whole grains.


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