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mshaw21
I think i just


Registered: 04/29/11
Posts: 774
Loc: Out of Town
Last seen: 7 years, 6 months
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Spore Syringe Half Used and Stored for Later?
#18424183 - 06/15/13 05:03 PM (11 years, 7 months ago) |
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I inoculated a couple jars and only used about half of my MS syringe. I put the cap back on the needle as soon as i was done.
Will my syringe be okay for later use or will it contaminate?
-------------------- crossed the line
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Borstili
Stranger
Registered: 05/16/11
Posts: 59
Last seen: 2 years, 16 days
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Re: Spore Syringe Half Used and Stored for Later? [Re: mshaw21]
#18424200 - 06/15/13 05:08 PM (11 years, 7 months ago) |
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For me this always worked. I just try to be sure to have it not contaminated, spraying the needle and the cap with alcohol and pushing a drop out before closing again.
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WolfieNuke
Professional Psychonaut



Registered: 12/21/09
Posts: 421
Loc: Washington, US
Last seen: 7 years, 6 months
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Re: Spore Syringe Half Used and Stored for Later? [Re: mshaw21]
#18424204 - 06/15/13 05:10 PM (11 years, 7 months ago) |
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Should be absolutely fine. I just wipe the exterior of the syringe with 70% isopropyl alcohol and re-flame the needle... No problems. I would clean the inside of the cap with alcohol as well before re-capping.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 24 minutes, 10 seconds
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Re: Spore Syringe Half Used and Stored for Later? [Re: WolfieNuke]
#18424217 - 06/15/13 05:14 PM (11 years, 7 months ago) |
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if you did pf jars flame the needle good before you put it away
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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mshaw21
I think i just


Registered: 04/29/11
Posts: 774
Loc: Out of Town
Last seen: 7 years, 6 months
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Re: Spore Syringe Half Used and Stored for Later? [Re: cronicr]
#18424225 - 06/15/13 05:17 PM (11 years, 7 months ago) |
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Thanks all! Im doing wbs atm, and ive never not used all of my syringe...until now
-------------------- crossed the line
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 3 hours, 45 minutes
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Re: Spore Syringe Half Used and Stored for Later? [Re: mshaw21]
#18424396 - 06/15/13 06:07 PM (11 years, 7 months ago) |
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ya i would still flame sterilize
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migraineur
Geezer


Registered: 02/15/10
Posts: 1,642
Last seen: 1 year, 5 months
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Re: Spore Syringe Half Used and Stored for Later? [Re: mshaw21]
#18426608 - 06/16/13 08:21 AM (11 years, 6 months ago) |
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There is no need to wipe your syringe's needle with an alcohol soaked paper towel etc. You could end up wiping germs onto it. A hot burning flame from your lighter will kill those germs.
Put your syringe in a clean ziplock bag or something and store it in the fridge. The same goes for liquid cultures that you aren't using.
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WolfieNuke
Professional Psychonaut



Registered: 12/21/09
Posts: 421
Loc: Washington, US
Last seen: 7 years, 6 months
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Re: Spore Syringe Half Used and Stored for Later? [Re: migraineur]
#18426784 - 06/16/13 09:26 AM (11 years, 6 months ago) |
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My suggestion was to wipe the exterior of the syringe - not the needle... I don't know if this is necessary, but I do this anyway.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 24 minutes, 10 seconds
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Re: Spore Syringe Half Used and Stored for Later? [Re: mshaw21]
#18426822 - 06/16/13 09:40 AM (11 years, 6 months ago) |
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Quote:
6DEA6 said:
Quote:
mshaw21 said: I inoculated a couple jars and only used about half of my MS syringe. I put the cap back on the needle as soon as i was done.
Will my syringe be okay for later use or will it contaminate?
You may just want to rid of them since they are used only for microscopy use only 
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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