|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
jackal83
Stranger

Registered: 12/21/11
Posts: 42
Last seen: 10 years, 4 months
|
cloning &agar
#18302732 - 05/22/13 08:36 AM (11 years, 7 months ago) |
|
|
helloo,,I have ordered ,agar (not agar-agar) and also ordered malt syrup,,,,so can i clon on mix of agar and malt syrup((already do it)) AND question,,how many days i" can save mushrom sample for cloning -example i must pick mushrom today but i can clon it for couple days
|
FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
|
Re: cloning &agar [Re: jackal83]
#18302740 - 05/22/13 08:39 AM (11 years, 7 months ago) |
|
|
You, you can clone on agar. Use the tissue right away, don't store a fruit you intend to clone for later. You want young, fresh mycelium.
--------------------
Quick WBS Prep
|
fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
|
Re: cloning &agar [Re: FooMan]
#18303893 - 05/22/13 01:22 PM (11 years, 7 months ago) |
|
|
You can clone even from dried fruits that are old.
If you want the easiest time of it with the fastest growth and the healthiest myc you should clone before the tissue dries out though. Leaving the fruits for a few days won't make any difference though.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy
The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed
"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)
I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
|
cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 27 minutes, 8 seconds
|
Re: cloning &agar [Re: fastfred]
#18303951 - 05/22/13 01:32 PM (11 years, 7 months ago) |
|
|
hi jackal, good to see you again, your english is getting alot better my friend. for best results you want a the mushroom to be fresh but if you have to try put it in a paper bag in the fridge
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
|
FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
|
Re: cloning &agar [Re: cronicr]
#18304470 - 05/22/13 03:04 PM (11 years, 7 months ago) |
|
|
LOL, fred, I know you were just waiting for a post from me to put your 2 cents on 
I'm still sticking to my original advice. The fresher the better. Why the hell would anyone leave a fruit they intend to clone sitting for days? That's just silly talk
--------------------
Quick WBS Prep
|
jackal83
Stranger

Registered: 12/21/11
Posts: 42
Last seen: 10 years, 4 months
|
Re: cloning &agar [Re: FooMan]
#18314836 - 05/24/13 02:51 PM (11 years, 7 months ago) |
|
|
doooya my clones are grooving after 2 days on my agar ho made plates and what now,,how that myc on agar put in inject  everywhere are Teks about cloning this way cloning that way ,but what NOW?
|
cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 27 minutes, 8 seconds
|
Re: cloning &agar [Re: jackal83]
#18314848 - 05/24/13 02:54 PM (11 years, 7 months ago) |
|
|
are you asking where to go from there?
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
|
jackal83
Stranger

Registered: 12/21/11
Posts: 42
Last seen: 10 years, 4 months
|
Re: cloning &agar [Re: cronicr]
#18315356 - 05/24/13 05:07 PM (11 years, 7 months ago) |
|
|
yes? if helthy tissue grow on my agar what next,,
|
jackal83
Stranger

Registered: 12/21/11
Posts: 42
Last seen: 10 years, 4 months
|
Re: cloning &agar [Re: jackal83]
#18322096 - 05/26/13 07:22 AM (11 years, 7 months ago) |
|
|
CMON what next,,,my agar growing((i made mistake,,when i was pouting agar in jars ,i put 10 cm agar but hope that not wil be a problem,,,,
What your experts on agar ,,thinking that i isolate good mycelium,that tissue put in Small test jar and put that to colonized and than make myc water,,, i have 10 jars to inoculate,,,
OR HOW MAKE CLON TISSUE IN LIQUID CULTURE!!! PLEASE HELP
|
the_jerk
return Noob


Registered: 02/13/13
Posts: 105
Last seen: 4 months, 5 days
|
Re: cloning &agar [Re: jackal83]
#18322202 - 05/26/13 08:09 AM (11 years, 7 months ago) |
|
|
If you want to isolate I would take some of the bests bits of colonized agar and transfer to new agar dishes.
If you just want to grow it out on grains or something then let the agar colonize to about 90% then cut it into chunks like slives of pizza and put a few chunks in each of your grain jars.
After they have been transferred give your jars a gentle tumble to mix it in a bit.
Unless you're asking something completely different....
|
jackal83
Stranger

Registered: 12/21/11
Posts: 42
Last seen: 10 years, 4 months
|
Re: cloning &agar [Re: the_jerk]
#18353073 - 06/01/13 11:11 AM (11 years, 7 months ago) |
|
|
hmmmmmm,,,,,in my plates ,,are forming green mold,,, then i was with scalper transfer healthy mycelium,in new dishes(improvised ya rs) then now is growing and ,again is green ,,,now wil try next transfer ,,but whil try with hydrogen peroxide 3%,, i am working in improvised glow box,, hope that 3.transfer wh il result with healthy mycelium
|
jackal83
Stranger

Registered: 12/21/11
Posts: 42
Last seen: 10 years, 4 months
|
Re: cloning &agar [Re: jackal83]
#18353231 - 06/01/13 11:48 AM (11 years, 7 months ago) |
|
|
sory on the double posts,,, but is ther good TEKs for transfering&geting clean sample,,,,
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 27 days
|
Re: cloning &agar [Re: jackal83]
#18353243 - 06/01/13 11:51 AM (11 years, 7 months ago) |
|
|
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
|
jackal83
Stranger

Registered: 12/21/11
Posts: 42
Last seen: 10 years, 4 months
|
|
helo i Finlay have healthy mycelium growing on agar "jars"" can i put water in that yar and suck back the mycelium in syringe than inoculate with that syringe ,,,,,because i am in fear of , opening yars and get ing contaminated
|
the_jerk
return Noob


Registered: 02/13/13
Posts: 105
Last seen: 4 months, 5 days
|
Re: cloning &agar [Re: jackal83]
#18362432 - 06/03/13 01:02 PM (11 years, 7 months ago) |
|
|
Save yourself a lot of time and effort and build a simple still-air box.
Sterilite 66QT all clear tote with clip on lid. Cut two 4" holes for hands.
Then you can let the agar colonize till near the edges and cut into wedges and drop wedges into your jars.
If you're going to try the water method - and I dont suggest that - make sure you have sterile water and a sterile syringe and preferably a RTV silicone "self-healing injection port" on the lid of your jar. That way you dont have to open it to get water in and out.
Since you had contams, I'd suggest one more transfer to a new jar to make sure theres no contams left for sure.
|
jackal83
Stranger

Registered: 12/21/11
Posts: 42
Last seen: 10 years, 4 months
|
Re: cloning &agar [Re: the_jerk]
#18422946 - 06/15/13 11:41 AM (11 years, 7 months ago) |
|
|
OK,guys i was transfer clean agar ((hmmm:)) clone isolate, in Small yar100grams, yar was fully colonize,then I was,knocked that yar with 40milli tars sterile water,sucked up water and make CLONE GRAIN LIQUID CULTURE ,,,and then knocked up 10 yars(350 grams) and NOW are all at 90 -100 % colonized,,,,,,,,NO CONTAMS ,but for all be 100% i was SHAKE all 10 yars and put them to 100% colonization again to see did all clean,, NOW til waiting loking to some god monotub teks !!!??? and in the chamber wait ng mini bulk spawn with 500grams of rye and a wery Little mix of coco and werm,,
Ps:all my yar lid was self heling with 1 litle hhole that was filtered with litle pice of tywek and a micropore-trak
SO SHROMERY SITE &AND YOU SHROMERY GUYS BIG THANX ,,ALLL MY EXPERIENCE WAS FROM THIS SITE THANX AGAIN
Edited by jackal83 (06/15/13 11:43 AM)
|
invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
|
Re: cloning &agar [Re: jackal83]
#18423025 - 06/15/13 12:12 PM (11 years, 7 months ago) |
|
|
Your idea with using 3% hydrogen peroxide was not too far off. You can use peroxide but it has to be at the right concentration.
this is discussed at length in this online book: (despite the title talking about sawdust there is a chapter that talks about agar and hydrogen peroxide)
Non-Sterile Mushroom Cultivation Growing Mushrooms on Nitrogen-enriched Sawdust without Sterilization Copyright © 2004 R. Rush Wayne. Ph.D.
|
jackal83
Stranger

Registered: 12/21/11
Posts: 42
Last seen: 10 years, 4 months
|
Re: cloning &agar [Re: invitro]
#18423450 - 06/15/13 02:09 PM (11 years, 7 months ago) |
|
|
and yes ,,wheN i WAS ADED PEROXIDE NIETHER AGAR YAR DIDNT CONTAMINED !!!!!!!!
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 27 days
|
Re: cloning &agar [Re: jackal83]
#18423458 - 06/15/13 02:13 PM (11 years, 7 months ago) |
|
|
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (06/15/13 02:13 PM)
|
|