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Offlinetlien
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Registered: 10/07/12
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Last seen: 10 years, 3 months
Likelihood of success? Iffy spore syringe?
    #18419022 - 06/14/13 01:56 PM (11 years, 7 months ago)

Hi everybody,

I overheard some stranger talking about growing mushrooms.

She said she'd gotten a spore print from somebody, stored it for almost 12 months in a sealed and undisturbed ziplock bag in a dark box, then made a syringe from that sample, by boiling water for 15 minutes, sucking up the water and emptying it several times, then allowing the sterile syringe cool down, before using alcohol sterilized hands in an alcohol sterilized environment to open and unwrap the print, scraping gently at it with a home made loop (paper clip), which was of course flame sterilized, then expelling the sterile water from the syringe onto the sample, stirring it, and sucking it back into the syringe.

That syringe was then stored for 6 months at room temperature, before being injected into sterile cakes.

I have four reasons to be fear whether that person would succeed with this approach.

1) 12+ months on sealed foil then 6 months in syringe.. too long? dead spores?

2) When she sucked the spores into the syringe, there was zero visible signs of spores being present in the solution. Should one see clearly a lot of grime or "dust" in there? There was nothing... Does one have to scrape vigorously to get the spores to release from the foil?

3) Due to unavailability of proper jars with lids, she had to use glasses and two layers of foil. One layer was removed at the time of injection, then that same layer was added after the four punctures had been made. Iffy?

4) Unfortunately, the 6 glasses with the foil layers had to be put into a plastic bag, and taken for a two hour drive only two days after injection of the spores. How likely is this to mess something up?

Supposedly, these glasses are currently resting quietly in the dark, so she will see after some weeks what's up, but it would be cool to hear if anybody has any views on these things...

Worst case scenario, she'll get 6 glasses filled with mold. Throw that out, do it again properly. She'd probably like some input :laugh:


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InvisibleThadeous
On the path

Registered: 08/02/11
Posts: 1,101
Re: Likelihood of success? Iffy spore syringe? [Re: tlien]
    #18419049 - 06/14/13 02:05 PM (11 years, 7 months ago)

1) AFAIK that is an acceptable length of time.

2) An individula spore is too small for the eye to see.  When you see clumps in other syringes, you're looking at billions of them.  When you scrapped the foil, they do come off it, so you should be able to see the foil beneath them.  It didn't take too much when I did it, and I used a needle.

3) Sounds like you're inviting contaminates in, but, no spore print is clean anywho.. You'd have to inoculate some jars to find out.

4) See #3.

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OfflinePussyFart
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Last seen: 7 months, 27 days
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Re: Likelihood of success? Iffy spore syringe? [Re: tlien]
    #18419055 - 06/14/13 02:06 PM (11 years, 7 months ago)

Quote:

tlien said:
1) 12+ months on sealed foil then 6 months in syringe.. too long? dead spores?



Not likely, they are most likely fine.

Quote:

tlien said:
2) When she sucked the spores into the syringe, there was zero visible signs of spores being present in the solution. Should one see clearly a lot of grime or "dust" in there? There was nothing... Does one have to scrape vigorously to get the spores to release from the foil?



Spores are microscopic and cannot be seen by the naked eye.

A light syringe is a good syringe. Dark syringes are not needed.

Quote:

tlien said:
3) Due to unavailability of proper jars with lids, she had to use glasses and two layers of foil. One layer was removed at the time of injection, then that same layer was added after the four punctures had been made. Iffy?



I assume you are talking about PF Tek cakes, in which case there is a dry verm layer which is the contam barrier.

Foil as a lid, not iffy...

Quote:

tlien said:
4) Unfortunately, the 6 glasses with the foil layers had to be put into a plastic bag, and taken for a two hour drive only two days after injection of the spores. How likely is this to mess something up?



Did they get jarred around?

Is the dry verm layer still intact?

Quote:

tlien said:
Supposedly, these glasses are currently resting quietly in the dark, so she will see after some weeks what's up, but it would be cool to hear if anybody has any views on these things...



Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

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Offlinetlien
Male

Registered: 10/07/12
Posts: 378
Last seen: 10 years, 3 months
Re: Likelihood of success? Iffy spore syringe? [Re: PussyFart]
    #18419072 - 06/14/13 02:12 PM (11 years, 7 months ago)

Thanks for the info, the method and circumstances were not optimal, but there is some hope then. A dry verm layer was applied, although it was possible too thin. Also, there is a definite risk of some jarring.. There are things to improve on for the future, definitely.

She was under the impression that an inoculated jar/glass was to be simply shoved into a dark closed and given a few weeks.

Just to be clear, they should be allowed some sun shine for about 12 hrs a day? These freshly inoculated glasses? There's no access to proper spectrum artificial light, no problem to stealthily give some sunlight though, that's no problem at all.


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Edited by tlien (06/14/13 02:13 PM)

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OfflineBloodKil
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Registered: 03/16/13
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Last seen: 6 months, 12 days
Re: Likelihood of success? Iffy spore syringe? [Re: PussyFart]
    #18419095 - 06/14/13 02:19 PM (11 years, 7 months ago)

Pretty much everything hacker said is what you want to go by... 

However, the next time your standing next to a complete stranger and they are blabbing all about how they are growing their shrooms, you should slap them for being stupid.

If however this was simply some clever ploy at attempting plausible deny ability, I invite you to look at how our justice system is set up, and what can be used as evidence against you.  Your truly not disguising anything by doing so.  In fact if I was a cop, I would be adding charges of conspiracy to comit with some of the foaf stuff I see on here.  (If for nothing else but the laughs and adding an additional charge they will need to plead away from if busted)



Edit doesn't have to be sunshine necessarily (though free light is better) but yes try to keep them on a 12/12 schedual through the entire grow cycle.

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Offlinetlien
Male

Registered: 10/07/12
Posts: 378
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Re: Likelihood of success? Iffy spore syringe? [Re: BloodKil]
    #18419107 - 06/14/13 02:21 PM (11 years, 7 months ago)

Hahaha, quite. You have some good points, BloodKil.

Your advice is noted, thanks a lot, people.


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OfflinePussyFart
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Re: Likelihood of success? Iffy spore syringe? [Re: BloodKil]
    #18419109 - 06/14/13 02:21 PM (11 years, 7 months ago)

Not direct sunlight, ambient/indirect light....like on a shelf in a room that gets natural light.(not darkness)

Edited by PussyFart (06/14/13 02:22 PM)

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Offlinecubenpete
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Registered: 03/13/12
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Re: Likelihood of success? Iffy spore syringe? [Re: BloodKil]
    #18419210 - 06/14/13 02:51 PM (11 years, 7 months ago)

Quote:

BloodKil said:

However, the next time your standing next to a complete stranger and they are blabbing all about how they are growing their shrooms, you should slap them for being stupid.






:lmafo:

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Offlinetlien
Male

Registered: 10/07/12
Posts: 378
Last seen: 10 years, 3 months
Re: Likelihood of success? Iffy spore syringe? [Re: cubenpete]
    #18422763 - 06/15/13 10:41 AM (11 years, 7 months ago)

Hey guys. Now, this woman I overheard (I'm continuing this goofy schtick, and you can't stop me), well she also showed some pictures haha.

Right: I know these pics are weak, but please tell me, in your best judgement, is this growth of mold, or growth of mycelium? It's been.. hmm.. 4 days?



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Offlinetlien
Male

Registered: 10/07/12
Posts: 378
Last seen: 10 years, 3 months
Re: Likelihood of success? Iffy spore syringe? [Re: tlien]
    #18422777 - 06/15/13 10:46 AM (11 years, 7 months ago)

F5 F5 F5 F5 F5 F5 F5 F5 F5 F5 F5 F5

:laugh:


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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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OG Cultivator
Re: Likelihood of success? Iffy spore syringe? [Re: tlien]
    #18422783 - 06/15/13 10:47 AM (11 years, 7 months ago)

Quote:

tlien said:
Hey guys. Now, this woman I overheard (I'm continuing this goofy schtick, and you can't stop me)




No, but I can skip over your question because that's annoying.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Offlinetlien
Male

Registered: 10/07/12
Posts: 378
Last seen: 10 years, 3 months
Re: Likelihood of success? Iffy spore syringe? [Re: 36fuckin5]
    #18422791 - 06/15/13 10:50 AM (11 years, 7 months ago)

Quote:

36fuckin5 said:
Quote:

tlien said:
Hey guys. Now, this woman I overheard (I'm continuing this goofy schtick, and you can't stop me)




No, but I can skip over your question because that's annoying.




Charming. Anybody else? Keen to see what's cooking in this little glass :wink:


--------------------



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Offlineveda_sticks
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Re: Likelihood of success? Iffy spore syringe? [Re: tlien]
    #18423018 - 06/15/13 12:10 PM (11 years, 7 months ago)

Quote:

tlien said:
Hi everybody,

I overheard some stranger talking about growing mushrooms.

She said she'd gotten a spore print from somebody, stored it for almost 12 months in a sealed and undisturbed ziplock bag in a dark box, then made a syringe from that sample, by boiling water for 15 minutes, sucking up the water and emptying it several times, then allowing the sterile syringe cool down, before using alcohol sterilized hands in an alcohol sterilized environment to open and unwrap the print, scraping gently at it with a home made loop (paper clip), which was of course flame sterilized, then expelling the sterile water from the syringe onto the sample, stirring it, and sucking it back into the syringe.

That syringe was then stored for 6 months at room temperature, before being injected into sterile cakes.

I have four reasons to be fear whether that person would succeed with this approach.

1) 12+ months on sealed foil then 6 months in syringe.. too long? dead spores?

2) When she sucked the spores into the syringe, there was zero visible signs of spores being present in the solution. Should one see clearly a lot of grime or "dust" in there? There was nothing... Does one have to scrape vigorously to get the spores to release from the foil?

3) Due to unavailability of proper jars with lids, she had to use glasses and two layers of foil. One layer was removed at the time of injection, then that same layer was added after the four punctures had been made. Iffy?

4) Unfortunately, the 6 glasses with the foil layers had to be put into a plastic bag, and taken for a two hour drive only two days after injection of the spores. How likely is this to mess something up?

Supposedly, these glasses are currently resting quietly in the dark, so she will see after some weeks what's up, but it would be cool to hear if anybody has any views on these things...

Worst case scenario, she'll get 6 glasses filled with mold. Throw that out, do it again properly. She'd probably like some input :laugh:





Older prints are generally recomended to start on agar. As spores age the number of viable spores goes down. However i have and many others have had good success using an old print straight to a syringe and then used to inoculate cakes. Storing a syringe for 6 months is fine it should still be viable.

I used a 2 year old print that was gifted to me (from a spore giveaway) and that worked just fine, i did have a couple of jars that did nothing but most of the jars colinised were spawned to bulk and have fruited/in the proccess of fruiting.

Moving pf jars around isnt recomended. The dry verm layer which is your filter if it gets disturbed could let any contaminants that it cought filter through, but you should be fine.

Make sure that your 2nd layer of foil is loose so as to allow proper gas exchange

No need to keep them in the dark, infact most people recomend having exposure to ambient lighting during colinisation, i certainly seemed to notice improvements.

www.mushroomvideos.com


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinetlien
Male

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Re: Likelihood of success? Iffy spore syringe? [Re: veda_sticks]
    #18423074 - 06/15/13 12:28 PM (11 years, 7 months ago)

Thanks a lot Veda, this first attempt is certainly not optimal, I have received a lot of advice on what to do and what not to do.

Next time I will be sure to have a thicker layer of dry verm, I fear this one was very thin, I didn't quite understand it's purpose at the time.

In fact, I also used WHITE rice flour, I think, it's not as dark as the types I've seen people post pictures of, but it's what I had available. I have some whole rye, perhaps I will try that next if this fails.

I'll loosen the top lids slightly, and keep allowing them natural light exposure.

Would you be able to comment on the two pictures I posted, and in your best judgement determine if this is mold or mycelium?

I would very much appreciate it :wink:

Worst case scenario I throw this batch out and give it another go, it's a very enjoyable experiment regardless of the outcome!


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